Vesiclepedia: EVQuant results

Results shown for selected experiment:

Experiment ID 36

PubMed ID 33638284

Identification method Protein

Extracellular vesicle type Microvesicles

MISEV Large EVs

Species Canis lupus familiaris

Experiment description Transglutaminase-2, RNA-binding proteins and mitochondrial proteins selectively traffic to MDCK cell-derived microvesicles following H-Ras-induced epithelial-mesenchymal transition

Sample Kidney cells

Sample name MDCK (21D1) - 10K pellet

Isolation/purification methods Differential centrifugation

Methods used in the study Western blotting
Mass spectrometry

Flotation density -

Authors Shafiq A, Suwakulsiri W, Rai A, Chen M, Greening DW, Zhu HJ, Xu R, Simpson RJ.

Journal name Proteomics

Publication year 2021

EV-TRACK -

   Contents identified in selected experiment:

   Top 200 abundant gene(s) for selected experiment:

   Other experiments from same study (PubMed ID):

PubMed ID Species Experiment description Sample Sample name Identifications Isolation methods Vesicle type MISEV Quantification

1 33638284 Canis lupus familiaris Transglutaminase-2, RNA-binding proteins and mitochondrial proteins selectively traffic to MDCK cell-derived microvesicles following H-Ras-induced epithelial-mesenchymal transition Kidney cells MDCK - 10K pellet Protein Differential centrifugation Microvesicles Large EVs EVQuant

2 33638284 Canis lupus familiaris Transglutaminase-2, RNA-binding proteins and mitochondrial proteins selectively traffic to MDCK cell-derived microvesicles following H-Ras-induced epithelial-mesenchymal transition Kidney cells MDCK (21D1) - 10K pellet Protein Differential centrifugation Microvesicles Large EVs EVQuant

   Experiments in which CD44 is identified:

PubMed ID Species Experiment description Sample Sample name Identifications Isolation methods Vesicle type MISEV Quantification

1 33638284 Canis lupus familiaris Transglutaminase-2, RNA-binding proteins and mitochondrial proteins selectively traffic to MDCK cell-derived microvesicles following H-Ras-induced epithelial-mesenchymal transition Kidney cells MDCK - 10K pellet Protein Differential centrifugation Microvesicles Large EVs EVQuant

2 33638284 Canis lupus familiaris Transglutaminase-2, RNA-binding proteins and mitochondrial proteins selectively traffic to MDCK cell-derived microvesicles following H-Ras-induced epithelial-mesenchymal transition Kidney cells MDCK (21D1) - 10K pellet Protein Differential centrifugation Microvesicles Large EVs EVQuant

	PubMed ID	Species	Experiment description	Sample	Sample name	Identifications	Isolation methods	Vesicle type	MISEV	Quantification
1	33638284	Canis lupus familiaris	Transglutaminase-2, RNA-binding proteins and mitochondrial proteins selectively traffic to MDCK cell-derived microvesicles following H-Ras-induced epithelial-mesenchymal transition	Kidney cells	MDCK - 10K pellet	Protein	Differential centrifugation	Microvesicles	Large EVs	EVQuant
2	33638284	Canis lupus familiaris	Transglutaminase-2, RNA-binding proteins and mitochondrial proteins selectively traffic to MDCK cell-derived microvesicles following H-Ras-induced epithelial-mesenchymal transition	Kidney cells	MDCK (21D1) - 10K pellet	Protein	Differential centrifugation	Microvesicles	Large EVs	EVQuant