Gene description for Mfge8
Gene name	milk fat globule EGF and factor V/VIII domain containing
Gene symbol	Mfge8
Other names/aliases	MFG-E8 MP47 Mfgm P47 SED1 lactadherin
Species	Mus musculus
Experiment description of studies that identified Mfge8 in Epididymal epithelial cells
1 Experiment ID 2188 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 33792189    Organism Mus musculus Experiment description A novel role for milk fat globule-EGF factor 8 protein (MFGE8) in the mediation of mouse sperm-extracellular vesicle interactions Authors Trigg NA, Stanger SJ, Zhou W, Skerrett-Byrne DA, Sipilä P, Dun MD, Eamens AL, De Iuliis GN, Bromfield EG, Roman SD, Nixon B. Journal name Proteomics Publication year 2021 Sample Epididymal epithelial cells Sample name mECap18 - Fraction 9 Isolation/purification methods Filtration Tangential flow filtration OptiPrep density gradient centrifugation Ultracentrifugation Centrifugal ultrafiltration Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
2 Experiment ID 1994 Identified molecule Protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 34188787    Organism Mus musculus Experiment description Arrdc4-dependent extracellular vesicle biogenesis is required for sperm maturation Authors Foot NJ, Gonzalez MB, Gembus K, Fonseka P, Sandow JJ, Nguyen TT, Tran D, Webb AI, Mathivanan S, Robker RL, Kumar S. Journal name J Extracell Vesicles Publication year 2021 Sample Epididymal epithelial cells Sample name CBA/CaH male mice Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry EV-TRACK -
3 Experiment ID 1994 Identified molecule Protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 34188787    Organism Mus musculus Experiment description Arrdc4-dependent extracellular vesicle biogenesis is required for sperm maturation Authors Foot NJ, Gonzalez MB, Gembus K, Fonseka P, Sandow JJ, Nguyen TT, Tran D, Webb AI, Mathivanan S, Robker RL, Kumar S. Journal name J Extracell Vesicles Publication year 2021 Sample Epididymal epithelial cells Sample name CBA/CaH male mice Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry EV-TRACK -
4 Experiment ID 1995 Identified molecule Protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 34188787    Organism Mus musculus Experiment description Arrdc4-dependent extracellular vesicle biogenesis is required for sperm maturation Authors Foot NJ, Gonzalez MB, Gembus K, Fonseka P, Sandow JJ, Nguyen TT, Tran D, Webb AI, Mathivanan S, Robker RL, Kumar S. Journal name J Extracell Vesicles Publication year 2021 Sample Epididymal epithelial cells Sample name CBA/CaH male mice Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry EV-TRACK -
5 Experiment ID 1995 Identified molecule Protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 34188787    Organism Mus musculus Experiment description Arrdc4-dependent extracellular vesicle biogenesis is required for sperm maturation Authors Foot NJ, Gonzalez MB, Gembus K, Fonseka P, Sandow JJ, Nguyen TT, Tran D, Webb AI, Mathivanan S, Robker RL, Kumar S. Journal name J Extracell Vesicles Publication year 2021 Sample Epididymal epithelial cells Sample name CBA/CaH male mice Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry EV-TRACK -