Vesiclepedia: EVQuant results

Results shown for selected experiment:

Experiment ID 11

PubMed ID 30863515

Identification method Protein

Extracellular vesicle type Extracellular vesicles

MISEV Small EVs

Species Homo sapiens

Experiment description Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles

Sample Lung epithelial cells

Sample name Control129

Isolation/purification methods Centrifugal ultrafiltration
Size exclusion chromatography

Methods used in the study Flow cytometry
Mass spectrometry

Flotation density -

Authors Benedikter BJ, Bouwman FG, Heinzmann ACA, Vajen T, Mariman EC, Wouters EFM, Savelkoul PHM, Koenen RR, Rohde GGU, van Oerle R, Spronk HM, Stassen FRM.

Journal name J Extracell Vesicles

Publication year 2019

EV-TRACK -

   Contents identified in selected experiment:

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Gene name Sample name Value EV Quant
A2M C126 ,CSE129 ,C127 ,CSE130 ,C129 ,C130 ,CSE127 ,CSE131 ,CSE128 2825,3683,3155,3575,2989,2353,3893,3668,4462 EVQuant
AARS1 C127 ,CSE131 ,CSE130 ,C129 ,C130 ,CSE127 ,CSE128 ,C126 ,CSE129 167,178,182,146,188,180,172,141,142 EVQuant
ACACA C130 ,CSE127 ,CSE128 ,C126 ,CSE129 ,C127 ,CSE131 ,CSE130 ,C129 196,143,174,170,248,194,174,217,215 EVQuant
ACAN CSE128 ,C126 ,CSE129 ,C127 ,CSE131 ,CSE130 ,C129 ,C130 ,CSE127 710,606,806,817,577,916,602,581,850 EVQuant
ACLY C126 ,CSE129 ,C127 ,CSE130 ,C129 ,C130 ,CSE127 ,CSE131 ,CSE128 3952,2886,3465,3255,3923,3686,3279,3525,3099 EVQuant
ACO1 CSE128 ,C126 ,CSE129 ,C127 ,CSE131 ,C129 ,C130 ,CSE130 ,CSE127 19,18,11,6,28,0,12,0,0 EVQuant
ACOT7 CSE128 ,C126 ,CSE129 ,C127 ,CSE131 ,C129 ,C130 ,CSE130 ,CSE127 0,0,0,0,0,0,0,0,0 EVQuant
ACTA2 C130 ,CSE127 ,CSE131 ,CSE128 ,C126 ,CSE129 ,C127 ,CSE130 ,C129 1506,957,1472,1264,1490,951,1500,1309,1573 EVQuant
ACTB CSE127 ,CSE131 ,CSE128 ,C126 ,CSE129 ,C127 ,CSE130 ,C129 ,C130 8684,14619,12167,13096,9307,14586,11996,16229,15001 EVQuant
ACTL6A CSE127 ,CSE128 ,C126 ,CSE129 ,C127 ,CSE131 ,CSE130 ,C129 ,C130 34,62,46,55,72,83,57,78,47 EVQuant
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   Top 200 abundant gene(s) for selected experiment:

   Other experiments from same study (PubMed ID):

PubMed ID Species Experiment description Sample Sample name Identifications Isolation methods Vesicle type MISEV Quantification

1 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control126 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

2 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE130 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

3 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE127 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

4 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE129 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

5 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE131 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

6 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control129 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

7 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE128 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

8 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control128 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

9 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control127 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

10 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control130 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

   Experiments in which CD63 is identified:

PubMed ID Species Experiment description Sample Sample name Identifications Isolation methods Vesicle type MISEV Quantification

1 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control126 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

2 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE130 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

3 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-Cas9-WT Protein Differential centrifugation
Filtration
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

4 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE127 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

5 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-KO-LAMP2A Protein Differential centrifugation
Filtration
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

6 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE129 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

7 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE131 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

8 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control129 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

9 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE128 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

10 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control127 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

11 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control130 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

	PubMed ID	Species	Experiment description	Sample	Sample name	Identifications	Isolation methods	Vesicle type	MISEV	Quantification
1	30863515	Homo sapiens	Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles	Lung epithelial cells	Control126	Protein	Centrifugal ultrafiltration Size exclusion chromatography	Extracellular vesicles	Small EVs	EVQuant
2	30863515	Homo sapiens	Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles	Lung epithelial cells	CSE130	Protein	Centrifugal ultrafiltration Size exclusion chromatography	Extracellular vesicles	Small EVs	EVQuant
3	30863515	Homo sapiens	Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles	Lung epithelial cells	CSE127	Protein	Centrifugal ultrafiltration Size exclusion chromatography	Extracellular vesicles	Small EVs	EVQuant
4	30863515	Homo sapiens	Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles	Lung epithelial cells	CSE129	Protein	Centrifugal ultrafiltration Size exclusion chromatography	Extracellular vesicles	Small EVs	EVQuant
5	30863515	Homo sapiens	Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles	Lung epithelial cells	CSE131	Protein	Centrifugal ultrafiltration Size exclusion chromatography	Extracellular vesicles	Small EVs	EVQuant
6	30863515	Homo sapiens	Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles	Lung epithelial cells	Control129	Protein	Centrifugal ultrafiltration Size exclusion chromatography	Extracellular vesicles	Small EVs	EVQuant
7	30863515	Homo sapiens	Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles	Lung epithelial cells	CSE128	Protein	Centrifugal ultrafiltration Size exclusion chromatography	Extracellular vesicles	Small EVs	EVQuant
8	30863515	Homo sapiens	Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles	Lung epithelial cells	Control128	Protein	Centrifugal ultrafiltration Size exclusion chromatography	Extracellular vesicles	Small EVs	EVQuant
9	30863515	Homo sapiens	Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles	Lung epithelial cells	Control127	Protein	Centrifugal ultrafiltration Size exclusion chromatography	Extracellular vesicles	Small EVs	EVQuant
10	30863515	Homo sapiens	Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles	Lung epithelial cells	Control130	Protein	Centrifugal ultrafiltration Size exclusion chromatography	Extracellular vesicles	Small EVs	EVQuant