Vesiclepedia: EVQuant results

Results shown for selected experiment:

Experiment ID 27

PubMed ID 35333565

Identification method Protein

Extracellular vesicle type Small extracellular vesicles

MISEV Small EVs

Species Homo sapiens

Experiment description LAMP2A regulates the loading of proteins into exosomes

Sample Retinal pigment epithelial cells

Sample name ARPE-19-Cas9-WT

Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation

Methods used in the study Western blotting
Mass spectrometry

Flotation density -

Authors Ferreira JV, da Rosa Soares A, Ramalho J, MÃƒÆ’Ã‚Â¡ximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, GirÃƒÆ’Ã‚Â£o H, van Niel G, Pereira P

Journal name Sci Adv

Publication year 2022

EV-TRACK -

   Contents identified in selected experiment:

   Top 200 abundant gene(s) for selected experiment:

   Other experiments from same study (PubMed ID):

PubMed ID Species Experiment description Sample Sample name Identifications Isolation methods Vesicle type MISEV Quantification

1 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-Cas9-WT Protein Differential centrifugation
Filtration
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

2 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-KO-LAMP2A Protein Differential centrifugation
Filtration
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

   Experiments in which PRKCI is identified:

PubMed ID Species Experiment description Sample Sample name Identifications Isolation methods Vesicle type MISEV Quantification

1 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

2 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

3 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

4 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-Cas9-WT Protein Differential centrifugation
Filtration
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

5 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-KO-LAMP2A Protein Differential centrifugation
Filtration
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

	PubMed ID	Species	Experiment description	Sample	Sample name	Identifications	Isolation methods	Vesicle type	MISEV	Quantification
1	35333565	Homo sapiens	LAMP2A regulates the loading of proteins into exosomes	Retinal pigment epithelial cells	ARPE-19-Cas9-WT	Protein	Differential centrifugation Filtration Ultracentrifugation	Small extracellular vesicles	Small EVs	EVQuant
2	35333565	Homo sapiens	LAMP2A regulates the loading of proteins into exosomes	Retinal pigment epithelial cells	ARPE-19-KO-LAMP2A	Protein	Differential centrifugation Filtration Ultracentrifugation	Small extracellular vesicles	Small EVs	EVQuant