Results shown for selected experiment:


Experiment ID27
PubMed ID 35333565   
Identification method Protein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
SpeciesHomo sapiens
Experiment descriptionLAMP2A regulates the loading of proteins into exosomes
SampleRetinal pigment epithelial cells
Sample nameARPE-19-Cas9-WT
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Methods used in the studyWestern blotting
Mass spectrometry
Flotation density-
AuthorsFerreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year2022
EV-TRACK -



   Contents identified in selected experiment:





   Top 200 abundant gene(s) for selected experiment:




   Other experiments from same study (PubMed ID):



 PubMed IDSpeciesExperiment descriptionSampleSample nameIdentificationsIsolation methodsVesicle typeMISEVQuantification
1 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-Cas9-WT Protein Differential centrifugation
Filtration
Ultracentrifugation		 Small extracellular vesicles Small EVs EVQuant
2 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-KO-LAMP2A Protein Differential centrifugation
Filtration
Ultracentrifugation		 Small extracellular vesicles Small EVs EVQuant



   Experiments in which TMX1 is identified:



 PubMed IDSpeciesExperiment descriptionSampleSample nameIdentificationsIsolation methodsVesicle typeMISEVQuantification
1 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Smooth muscle cells Carotid artery-derived primary smooth muscle cells Protein Differential centrifugation
Ultracentrifugation		 Extracellular vesicles EVs EVQuant
2 33661579 Homo sapiens Pannexin-1 channel regulates nuclear content packaging into apoptotic bodies and their size T lymphoblast Jurkat T cells Protein Differential centrifugation Apoptotic bodies Large EVs EVQuant
3 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Pancreatic cancer cells PANC-1 - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography		 Exosomes Small EVs EVQuant
4 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Breast cancer cells MDA-MB-231 - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography		 Exosomes Small EVs EVQuant
5 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Smooth muscle cells Carotid artery-derived primary smooth muscle cells Protein Differential centrifugation
Ultracentrifugation		 Extracellular vesicles EVs EVQuant
6 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Breast cancer cells MDA-MB-231 - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation 		Exosomes Small EVs EVQuant
7 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Pancreatic cancer cells PANC-1 - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation 		Exosomes Small EVs EVQuant
8 33661579 Homo sapiens Pannexin-1 channel regulates nuclear content packaging into apoptotic bodies and their size T lymphoblast Jurkat T cells Protein Differential centrifugation Apoptotic bodies Large EVs EVQuant
9 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Embryonic kidney cells HEK293T - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography		 Exosomes Small EVs EVQuant
10 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-KO-LAMP2A Protein Differential centrifugation
Filtration
Ultracentrifugation		 Small extracellular vesicles Small EVs EVQuant
11 33661579 Homo sapiens Pannexin-1 channel regulates nuclear content packaging into apoptotic bodies and their size T lymphoblast Jurkat T cells Protein Differential centrifugation Apoptotic bodies Large EVs EVQuant
12 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-Cas9-WT Protein Differential centrifugation
Filtration
Ultracentrifugation		 Small extracellular vesicles Small EVs EVQuant
13 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Embryonic kidney cells HEK293T - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation 		Exosomes Small EVs EVQuant