Vesiclepedia: EVQuant results

Results shown for selected experiment:

Experiment ID 29

PubMed ID 32487994

Identification method Protein

Extracellular vesicle type Extracellular vesicles

MISEV Small EVs

Species Homo sapiens

Experiment description Plasma-derived extracellular vesicles from Plasmodium vivax patients signal spleen fibroblasts via NF-kB facilitating parasite cytoadherence

Sample Plasma

Sample name Healthy donors

Isolation/purification methods Centrifugation
Size exclusion chromatography

Methods used in the study Western blotting
Mass spectrometry
Flow cytometry

Flotation density -

Authors Toda H, Diaz-Varela M, Segui-Barber J, Roobsoong W, Baro B, Garcia-Silva S, Galiano A, GualdrÃƒÆ’Ã‚Â³n-LÃƒÆ’Ã‚Â³pez M, Almeida ACG, Brito MAM, de Melo GC, Aparici-Herraiz I, Castro-CavadÃƒÆ’Ã‚Âa C, Monteiro WM, BorrÃƒÆ’Ã‚Â s E, SabidÃƒÆ’Ã‚Â³ E, Almeida IC, Chojnacki J, Martinez-Picado J, Calvo M, Armengol P, Carmona-Fonseca J, Yasnot MF, Lauzurica R, Marcilla A, Peinado H, Galinski MR, Lacerda MVG, Sattabongkot J, Fernandez-Becerra C, Del Portillo HA.

Journal name Nat Commun

Publication year 2020

EV-TRACK -

   Contents identified in selected experiment:

   Top 200 abundant gene(s) for selected experiment:

   Other experiments from same study (PubMed ID):

PubMed ID Species Experiment description Sample Sample name Identifications Isolation methods Vesicle type MISEV Quantification

1 32487994 Homo sapiens Plasma-derived extracellular vesicles from Plasmodium vivax patients signal spleen fibroblasts via NF-kB facilitating parasite cytoadherence Plasma Plasmodium vivax (Malaria) patients Protein Centrifugation
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

2 32487994 Homo sapiens Plasma-derived extracellular vesicles from Plasmodium vivax patients signal spleen fibroblasts via NF-kB facilitating parasite cytoadherence Plasma Healthy donors Protein Centrifugation
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

   Experiments in which FN1 is identified:

PubMed ID Species Experiment description Sample Sample name Identifications Isolation methods Vesicle type MISEV Quantification

1 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

2 32487994 Homo sapiens Plasma-derived extracellular vesicles from Plasmodium vivax patients signal spleen fibroblasts via NF-kB facilitating parasite cytoadherence Plasma Healthy donors Protein Centrifugation
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

3 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

4 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Pancreatic cancer cells PANC-1 - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation Exosomes Small EVs EVQuant

5 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control129 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

6 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE128 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

7 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Breast cancer cells MDA-MB-231 - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography Exosomes Small EVs EVQuant

8 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE130 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

9 32487994 Homo sapiens Plasma-derived extracellular vesicles from Plasmodium vivax patients signal spleen fibroblasts via NF-kB facilitating parasite cytoadherence Plasma Plasmodium vivax (Malaria) patients Protein Centrifugation
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

10 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-Cas9-WT Protein Differential centrifugation
Filtration
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

11 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Pancreatic cancer cells PANC-1 - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography Exosomes Small EVs EVQuant

12 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Embryonic kidney cells HEK293T - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography Exosomes Small EVs EVQuant

13 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE131 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

14 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Breast cancer cells MDA-MB-231 - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation Exosomes Small EVs EVQuant

15 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE127 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

16 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-KO-LAMP2A Protein Differential centrifugation
Filtration
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

17 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control126 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

18 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Embryonic kidney cells HEK293T - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation Exosomes Small EVs EVQuant

19 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control130 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

20 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE129 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

21 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

22 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control127 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

	PubMed ID	Species	Experiment description	Sample	Sample name	Identifications	Isolation methods	Vesicle type	MISEV	Quantification
1	32487994	Homo sapiens	Plasma-derived extracellular vesicles from Plasmodium vivax patients signal spleen fibroblasts via NF-kB facilitating parasite cytoadherence	Plasma	Plasmodium vivax (Malaria) patients	Protein	Centrifugation Size exclusion chromatography	Extracellular vesicles	Small EVs	EVQuant
2	32487994	Homo sapiens	Plasma-derived extracellular vesicles from Plasmodium vivax patients signal spleen fibroblasts via NF-kB facilitating parasite cytoadherence	Plasma	Healthy donors	Protein	Centrifugation Size exclusion chromatography	Extracellular vesicles	Small EVs	EVQuant