Vesiclepedia: EVQuant results

Results shown for selected experiment:

Experiment ID 3

PubMed ID 29339034

Identification method Protein

Extracellular vesicle type Exosomes

MISEV Small EVs

Species Homo sapiens

Experiment description Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer

Sample Pancreatic duct fluid

Sample name From patients with intraductal papillary mucinous neoplasm patients (IPMN)

Isolation/purification methods Differential centrifugation
Ultracentrifugation

Methods used in the study Mass spectrometry

Flotation density -

Authors Zheng J, Hernandez JM, Doussot A, Bojmar L, Zambirinis CP, Costa-Silva B, van Beek EJAH, Mark MT, Molina H, Askan G, Basturk O, Gonen M, Kingham TP, Allen PJ, D'Angelica MI, DeMatteo RP, Lyden D, Jarnagin WR.

Journal name HPB

Publication year 2018

EV-TRACK -

   Contents identified in selected experiment:

   Top 200 abundant gene(s) for selected experiment:

   Other experiments from same study (PubMed ID):

PubMed ID Species Experiment description Sample Sample name Identifications Isolation methods Vesicle type MISEV Quantification

1 29339034 Homo sapiens Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer Pancreatic duct fluid From patients with benign pancreatic disease Protein Differential centrifugation
Ultracentrifugation Exosomes Small EVs EVQuant

2 29339034 Homo sapiens Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer Pancreatic duct fluid From patients with intraductal papillary mucinous neoplasm patients (IPMN) Protein Differential centrifugation
Ultracentrifugation Exosomes Small EVs EVQuant

3 29339034 Homo sapiens Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer Pancreatic duct fluid From patients with Pancreatic ductal adenocarcinoma Protein Differential centrifugation
Ultracentrifugation Exosomes Small EVs EVQuant

4 29339034 Homo sapiens Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer Pancreatic duct fluid From patients with IPMN with microinvasive cancer (IPMNca) Protein Differential centrifugation
Ultracentrifugation Exosomes Small EVs EVQuant

   Experiments in which LAMC2 is identified:

PubMed ID Species Experiment description Sample Sample name Identifications Isolation methods Vesicle type MISEV Quantification

1 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

2 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Pancreatic cancer cells PANC-1 - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography Exosomes Small EVs EVQuant

3 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Breast cancer cells MDA-MB-231 - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography Exosomes Small EVs EVQuant

4 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Breast cancer cells MDA-MB-231 - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation Exosomes Small EVs EVQuant

5 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Pancreatic cancer cells PANC-1 - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation Exosomes Small EVs EVQuant

6 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Embryonic kidney cells HEK293T - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography Exosomes Small EVs EVQuant

7 29339034 Homo sapiens Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer Pancreatic duct fluid From patients with intraductal papillary mucinous neoplasm patients (IPMN) Protein Differential centrifugation
Ultracentrifugation Exosomes Small EVs EVQuant

8 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

9 29339034 Homo sapiens Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer Pancreatic duct fluid From patients with Pancreatic ductal adenocarcinoma Protein Differential centrifugation
Ultracentrifugation Exosomes Small EVs EVQuant

10 29339034 Homo sapiens Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer Pancreatic duct fluid From patients with benign pancreatic disease Protein Differential centrifugation
Ultracentrifugation Exosomes Small EVs EVQuant

11 29339034 Homo sapiens Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer Pancreatic duct fluid From patients with IPMN with microinvasive cancer (IPMNca) Protein Differential centrifugation
Ultracentrifugation Exosomes Small EVs EVQuant

12 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

13 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Embryonic kidney cells HEK293T - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation Exosomes Small EVs EVQuant

	PubMed ID	Species	Experiment description	Sample	Sample name	Identifications	Isolation methods	Vesicle type	MISEV	Quantification
1	29339034	Homo sapiens	Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer	Pancreatic duct fluid	From patients with benign pancreatic disease	Protein	Differential centrifugation Ultracentrifugation	Exosomes	Small EVs	EVQuant
2	29339034	Homo sapiens	Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer	Pancreatic duct fluid	From patients with intraductal papillary mucinous neoplasm patients (IPMN)	Protein	Differential centrifugation Ultracentrifugation	Exosomes	Small EVs	EVQuant
3	29339034	Homo sapiens	Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer	Pancreatic duct fluid	From patients with Pancreatic ductal adenocarcinoma	Protein	Differential centrifugation Ultracentrifugation	Exosomes	Small EVs	EVQuant
4	29339034	Homo sapiens	Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer	Pancreatic duct fluid	From patients with IPMN with microinvasive cancer (IPMNca)	Protein	Differential centrifugation Ultracentrifugation	Exosomes	Small EVs	EVQuant