Vesiclepedia: EVQuant results

Results shown for selected experiment:

Experiment ID 32

PubMed ID 32938681

Identification method Protein

Extracellular vesicle type Extracellular vesicles

MISEV EVs

Species Homo sapiens

Experiment description Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis

Sample Smooth muscle cells

Sample name Carotid artery-derived primary smooth muscle cells

Isolation/purification methods Differential centrifugation
Ultracentrifugation

Methods used in the study Mass spectrometry

Flotation density -

Authors Rogers MA, Buffolo F, Schlotter F, Atkins SK, Lee LH, Halu A, Blaser MC, Tsolaki E, Higashi H, Luther K, Daaboul G, Bouten CVC, Body SC, Singh SA, Bertazzo S, Libby P, Aikawa M, Aikawa E.

Journal name Sci Adv

Publication year 2020

EV-TRACK -

   Contents identified in selected experiment:

   Top 200 abundant gene(s) for selected experiment:

   Other experiments from same study (PubMed ID):

PubMed ID Species Experiment description Sample Sample name Identifications Isolation methods Vesicle type MISEV Quantification

1 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Valvular interstitial cells Aortic valve-derived primary valvular interstial cells Protein Differential centrifugation
Ultracentrifugation Extracellular vesicles EVs EVQuant

2 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Smooth muscle cells Carotid artery-derived primary smooth muscle cells Protein Differential centrifugation
Ultracentrifugation Extracellular vesicles EVs EVQuant

3 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Smooth muscle cells Carotid artery-derived primary smooth muscle cells Protein Differential centrifugation
Ultracentrifugation Extracellular vesicles EVs EVQuant

4 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Valvular interstitial cells Aortic valve-derived primary valvular interstial cells Protein Differential centrifugation
Ultracentrifugation Extracellular vesicles EVs EVQuant

   Experiments in which HSPA1A is identified:

PubMed ID Species Experiment description Sample Sample name Identifications Isolation methods Vesicle type MISEV Quantification

1 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Smooth muscle cells Carotid artery-derived primary smooth muscle cells Protein Differential centrifugation
Ultracentrifugation Extracellular vesicles EVs EVQuant

2 33661579 Homo sapiens Pannexin-1 channel regulates nuclear content packaging into apoptotic bodies and their size T lymphoblast Jurkat T cells Protein Differential centrifugation Apoptotic bodies Large EVs EVQuant

3 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control126 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

4 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE130 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

5 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Smooth muscle cells Carotid artery-derived primary smooth muscle cells Protein Differential centrifugation
Ultracentrifugation Extracellular vesicles EVs EVQuant

6 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-Cas9-WT Protein Differential centrifugation
Filtration
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

7 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE127 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

8 33661579 Homo sapiens Pannexin-1 channel regulates nuclear content packaging into apoptotic bodies and their size T lymphoblast Jurkat T cells Protein Differential centrifugation Apoptotic bodies Large EVs EVQuant

9 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-KO-LAMP2A Protein Differential centrifugation
Filtration
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

10 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE129 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

11 33661579 Homo sapiens Pannexin-1 channel regulates nuclear content packaging into apoptotic bodies and their size T lymphoblast Jurkat T cells Protein Differential centrifugation Apoptotic bodies Large EVs EVQuant

12 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE131 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

13 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control129 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

14 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE128 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

15 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control127 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

16 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control130 Protein Centrifugal ultrafiltration
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

	PubMed ID	Species	Experiment description	Sample	Sample name	Identifications	Isolation methods	Vesicle type	MISEV	Quantification
1	32938681	Homo sapiens	Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis	Valvular interstitial cells	Aortic valve-derived primary valvular interstial cells	Protein	Differential centrifugation Ultracentrifugation	Extracellular vesicles	EVs	EVQuant
2	32938681	Homo sapiens	Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis	Smooth muscle cells	Carotid artery-derived primary smooth muscle cells	Protein	Differential centrifugation Ultracentrifugation	Extracellular vesicles	EVs	EVQuant
3	32938681	Homo sapiens	Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis	Smooth muscle cells	Carotid artery-derived primary smooth muscle cells	Protein	Differential centrifugation Ultracentrifugation	Extracellular vesicles	EVs	EVQuant
4	32938681	Homo sapiens	Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis	Valvular interstitial cells	Aortic valve-derived primary valvular interstial cells	Protein	Differential centrifugation Ultracentrifugation	Extracellular vesicles	EVs	EVQuant