Vesiclepedia: EVQuant results

Results shown for selected experiment:

Experiment ID 32

PubMed ID 32938681

Identification method Protein

Extracellular vesicle type Extracellular vesicles

MISEV EVs

Species Homo sapiens

Experiment description Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis

Sample Smooth muscle cells

Sample name Carotid artery-derived primary smooth muscle cells

Isolation/purification methods Differential centrifugation
Ultracentrifugation

Methods used in the study Mass spectrometry

Flotation density -

Authors Rogers MA, Buffolo F, Schlotter F, Atkins SK, Lee LH, Halu A, Blaser MC, Tsolaki E, Higashi H, Luther K, Daaboul G, Bouten CVC, Body SC, Singh SA, Bertazzo S, Libby P, Aikawa M, Aikawa E.

Journal name Sci Adv

Publication year 2020

EV-TRACK -

   Contents identified in selected experiment:

   Top 200 abundant gene(s) for selected experiment:

   Other experiments from same study (PubMed ID):

PubMed ID Species Experiment description Sample Sample name Identifications Isolation methods Vesicle type MISEV Quantification

1 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Valvular interstitial cells Aortic valve-derived primary valvular interstial cells Protein Differential centrifugation
Ultracentrifugation Extracellular vesicles EVs EVQuant

2 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Smooth muscle cells Carotid artery-derived primary smooth muscle cells Protein Differential centrifugation
Ultracentrifugation Extracellular vesicles EVs EVQuant

3 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Smooth muscle cells Carotid artery-derived primary smooth muscle cells Protein Differential centrifugation
Ultracentrifugation Extracellular vesicles EVs EVQuant

4 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Valvular interstitial cells Aortic valve-derived primary valvular interstial cells Protein Differential centrifugation
Ultracentrifugation Extracellular vesicles EVs EVQuant

   Experiments in which PAWR is identified:

PubMed ID Species Experiment description Sample Sample name Identifications Isolation methods Vesicle type MISEV Quantification

1 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

2 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Smooth muscle cells Carotid artery-derived primary smooth muscle cells Protein Differential centrifugation
Ultracentrifugation Extracellular vesicles EVs EVQuant

3 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Pancreatic cancer cells PANC-1 - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography Exosomes Small EVs EVQuant

4 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Breast cancer cells MDA-MB-231 - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography Exosomes Small EVs EVQuant

5 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Smooth muscle cells Carotid artery-derived primary smooth muscle cells Protein Differential centrifugation
Ultracentrifugation Extracellular vesicles EVs EVQuant

6 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Breast cancer cells MDA-MB-231 - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation Exosomes Small EVs EVQuant

7 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Pancreatic cancer cells PANC-1 - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation Exosomes Small EVs EVQuant

8 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Embryonic kidney cells HEK293T - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography Exosomes Small EVs EVQuant

9 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-KO-LAMP2A Protein Differential centrifugation
Filtration
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

10 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

11 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-Cas9-WT Protein Differential centrifugation
Filtration
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

12 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

13 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Embryonic kidney cells HEK293T - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation Exosomes Small EVs EVQuant

	PubMed ID	Species	Experiment description	Sample	Sample name	Identifications	Isolation methods	Vesicle type	MISEV	Quantification
1	32938681	Homo sapiens	Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis	Valvular interstitial cells	Aortic valve-derived primary valvular interstial cells	Protein	Differential centrifugation Ultracentrifugation	Extracellular vesicles	EVs	EVQuant
2	32938681	Homo sapiens	Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis	Smooth muscle cells	Carotid artery-derived primary smooth muscle cells	Protein	Differential centrifugation Ultracentrifugation	Extracellular vesicles	EVs	EVQuant
3	32938681	Homo sapiens	Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis	Smooth muscle cells	Carotid artery-derived primary smooth muscle cells	Protein	Differential centrifugation Ultracentrifugation	Extracellular vesicles	EVs	EVQuant
4	32938681	Homo sapiens	Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis	Valvular interstitial cells	Aortic valve-derived primary valvular interstial cells	Protein	Differential centrifugation Ultracentrifugation	Extracellular vesicles	EVs	EVQuant