Gene description for flot1
Gene name flotillin 1
Gene symbol flot1
Other names/aliases -
Species Salmo salar
 Experiment description of studies that identified flot1 in Leukocytes

1
Experiment ID781
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 29953723   
OrganismSalmo salar
Experiment descriptionStimulation of exosome release by extracellular DNA is conserved across multiple cell types
AuthorsIliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R.
Journal name FEBS J
Publication year2018
SampleLeukocytes
Sample nameHead kidney leukocytes (HKLs) - S. salar genomic DNA stimulated
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

2
Experiment ID760
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
Western blotting
PubMed ID 29953723   
OrganismSalmo salar
Experiment descriptionStimulation of exosome release by extracellular DNA is conserved across multiple cell types
AuthorsIliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R.
Journal name FEBS J
Publication year2018
SampleLeukocytes
Sample nameHead kidney leukocytes (HKLs) - ODN 2006PS stimulated
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

3
Experiment ID759
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 29953723   
OrganismSalmo salar
Experiment descriptionStimulation of exosome release by extracellular DNA is conserved across multiple cell types
AuthorsIliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R.
Journal name FEBS J
Publication year2018
SampleLeukocytes
Sample nameHead kidney leukocytes (HKLs)
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

4
Experiment ID762
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 29953723   
OrganismSalmo salar
Experiment descriptionStimulation of exosome release by extracellular DNA is conserved across multiple cell types
AuthorsIliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R.
Journal name FEBS J
Publication year2018
SampleLeukocytes
Sample nameHead kidney leukocytes (HKLs) - ODN 2006PS stimulated - Fraction 6
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Density gradient centrifugation
Flotation density1.09-1.20 g/mL
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

5
Experiment ID773
Identified moleculeprotein
Extracellular vesicle typeApoptotic bodies
MISEVEVs
Identification method Western blotting
PubMed ID 29953723   
OrganismSalmo salar
Experiment descriptionStimulation of exosome release by extracellular DNA is conserved across multiple cell types
AuthorsIliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R.
Journal name FEBS J
Publication year2018
SampleLeukocytes
Sample nameHead kidney leukocytes (HKLs)
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

6
Experiment ID774
Identified moleculeprotein
Extracellular vesicle typeApoptotic bodies
MISEVEVs
Identification method Western blotting
PubMed ID 29953723   
OrganismSalmo salar
Experiment descriptionStimulation of exosome release by extracellular DNA is conserved across multiple cell types
AuthorsIliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R.
Journal name FEBS J
Publication year2018
SampleLeukocytes
Sample nameHead kidney leukocytes (HKLs) - ODN 2006PS stimulated
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

7
Experiment ID775
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
MISEVEVs
Identification method Western blotting
PubMed ID 29953723   
OrganismSalmo salar
Experiment descriptionStimulation of exosome release by extracellular DNA is conserved across multiple cell types
AuthorsIliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R.
Journal name FEBS J
Publication year2018
SampleLeukocytes
Sample nameHead kidney leukocytes (HKLs)
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

8
Experiment ID776
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
MISEVEVs
Identification method Western blotting
PubMed ID 29953723   
OrganismSalmo salar
Experiment descriptionStimulation of exosome release by extracellular DNA is conserved across multiple cell types
AuthorsIliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R.
Journal name FEBS J
Publication year2018
SampleLeukocytes
Sample nameHead kidney leukocytes (HKLs) - ODN 2006PS stimulated
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

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