Gene description for HUWE1
Gene name HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1
Gene symbol HUWE1
Other names/aliases ARF-BP1
HECTH9
HSPC272
Ib772
LASU1
MRXST
MULE
URE-B1
UREB1
Species Homo sapiens
 Experiment description of studies that identified HUWE1 in Gastric cancer cells

1
Experiment ID1493
Identified moleculeProtein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 33991177   
OrganismHomo sapiens
Experiment descriptionCancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
AuthorsCarli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year2021
SampleGastric cancer cells
Sample nameMKN1 - 100K pellet
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

2
Experiment ID1494
Identified moleculeProtein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 33991177   
OrganismHomo sapiens
Experiment descriptionCancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
AuthorsCarli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year2021
SampleGastric cancer cells
Sample nameMKN1 - 100K pellet
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

3
Experiment ID1495
Identified moleculeProtein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 33991177   
OrganismHomo sapiens
Experiment descriptionCancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
AuthorsCarli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year2021
SampleGastric cancer cells
Sample nameMKN1 - 100K pellet
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

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