Gene description for FLOT1
Gene name	flotillin 1
Gene symbol	FLOT1
Other names/aliases	-
Species	Homo sapiens
Experiment description of studies that identified FLOT1 in Fibroblasts
1 Experiment ID 1899 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 36266345    Organism Homo sapiens Experiment description Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression Authors Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J. Journal name Nat Commun Publication year 2022 Sample Fibroblasts Sample name MRC-5 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry EV-TRACK -
2 Experiment ID 1900 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 36266345    Organism Homo sapiens Experiment description Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression Authors Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J. Journal name Nat Commun Publication year 2022 Sample Fibroblasts Sample name MRC-5 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry EV-TRACK -
3 Experiment ID 1901 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 36266345    Organism Homo sapiens Experiment description Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression Authors Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J. Journal name Nat Commun Publication year 2022 Sample Fibroblasts Sample name BJ Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
4 Experiment ID 1902 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 36266345    Organism Homo sapiens Experiment description Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression Authors Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J. Journal name Nat Commun Publication year 2022 Sample Fibroblasts Sample name BJ Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
5 Experiment ID 3038 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Western blotting PubMed ID 33893753    Organism Homo sapiens Experiment description Label-free quantitative proteomic analysis of extracellular vesicles released from fibroblasts derived from patients with spinal muscular atrophy Authors Roberto J, Poulin KL, Parks RJ, Vacratsis PO. Journal name Proteomics Publication year 2021 Sample Fibroblasts Sample name GM03813 - SMA type 2 Isolation/purification methods Centrifugation Polyethylene glycol-based precipitation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
6 Experiment ID 3040 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Western blotting PubMed ID 33893753    Organism Homo sapiens Experiment description Label-free quantitative proteomic analysis of extracellular vesicles released from fibroblasts derived from patients with spinal muscular atrophy Authors Roberto J, Poulin KL, Parks RJ, Vacratsis PO. Journal name Proteomics Publication year 2021 Sample Fibroblasts Sample name AG08498 - Normal Isolation/purification methods Centrifugation Polyethylene glycol-based precipitation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
7 Experiment ID 3041 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Western blotting PubMed ID 33893753    Organism Homo sapiens Experiment description Label-free quantitative proteomic analysis of extracellular vesicles released from fibroblasts derived from patients with spinal muscular atrophy Authors Roberto J, Poulin KL, Parks RJ, Vacratsis PO. Journal name Proteomics Publication year 2021 Sample Fibroblasts Sample name AG07095 - Normal Isolation/purification methods Centrifugation Polyethylene glycol-based precipitation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
8 Experiment ID 3042 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Western blotting PubMed ID 33893753    Organism Homo sapiens Experiment description Label-free quantitative proteomic analysis of extracellular vesicles released from fibroblasts derived from patients with spinal muscular atrophy Authors Roberto J, Poulin KL, Parks RJ, Vacratsis PO. Journal name Proteomics Publication year 2021 Sample Fibroblasts Sample name GM05565 - Normal Isolation/purification methods Centrifugation Polyethylene glycol-based precipitation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
9 Experiment ID 3043 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Western blotting PubMed ID 33893753    Organism Homo sapiens Experiment description Label-free quantitative proteomic analysis of extracellular vesicles released from fibroblasts derived from patients with spinal muscular atrophy Authors Roberto J, Poulin KL, Parks RJ, Vacratsis PO. Journal name Proteomics Publication year 2021 Sample Fibroblasts Sample name GM00232 - SMA type 1 Isolation/purification methods Centrifugation Polyethylene glycol-based precipitation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
10 Experiment ID 3044 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Western blotting PubMed ID 33893753    Organism Homo sapiens Experiment description Label-free quantitative proteomic analysis of extracellular vesicles released from fibroblasts derived from patients with spinal muscular atrophy Authors Roberto J, Poulin KL, Parks RJ, Vacratsis PO. Journal name Proteomics Publication year 2021 Sample Fibroblasts Sample name GM22592 - SMA type 2 Isolation/purification methods Centrifugation Polyethylene glycol-based precipitation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
11 Experiment ID 3036 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 33893753    Organism Homo sapiens Experiment description Label-free quantitative proteomic analysis of extracellular vesicles released from fibroblasts derived from patients with spinal muscular atrophy Authors Roberto J, Poulin KL, Parks RJ, Vacratsis PO. Journal name Proteomics Publication year 2021 Sample Fibroblasts Sample name GM03813 - SMA type 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -
12 Experiment ID 3037 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 33893753    Organism Homo sapiens Experiment description Label-free quantitative proteomic analysis of extracellular vesicles released from fibroblasts derived from patients with spinal muscular atrophy Authors Roberto J, Poulin KL, Parks RJ, Vacratsis PO. Journal name Proteomics Publication year 2021 Sample Fibroblasts Sample name GM03814 - Normal Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -