Gene description for FLOT1
Gene name	flotillin 1
Gene symbol	FLOT1
Other names/aliases	-
Species	Homo sapiens
Experiment description of studies that identified FLOT1 in T cells
1 Experiment ID 1004 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 23463506    Organism Homo sapiens Experiment description The intracellular interactome of tetraspanin-enriched microdomains reveals their function as sorting machineries toward exosomes. Authors Perez-Hernandez D, Gutiéez-Váuez C, Jorge I, Ló-MartíS, Ursa A, Sáhez-Madrid F, Váuez J, Yáz-Mó Journal name J Biol Chem. Publication year 2013 Sample T cells Sample name T lymphoblasts - exosomes pulldown with CD81 as a bait Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV130110: EV-METRIC:22%
2 Experiment ID 1008 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 23463506    Organism Homo sapiens Experiment description The intracellular interactome of tetraspanin-enriched microdomains reveals their function as sorting machineries toward exosomes. Authors Perez-Hernandez D, Gutiéez-Váuez C, Jorge I, Ló-MartíS, Ursa A, Sáhez-Madrid F, Váuez J, Yáz-Mó Journal name J Biol Chem. Publication year 2013 Sample T cells Sample name T lymphoblasts Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130110: EV-METRIC:22%
3 Experiment ID 802 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 29953723    Organism Homo sapiens Experiment description Stimulation of exosome release by extracellular DNA is conserved across multiple cell types Authors Iliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R. Journal name FEBS J Publication year 2018 Sample T cells Sample name Jurkat cells Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
4 Experiment ID 803 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 29953723    Organism Homo sapiens Experiment description Stimulation of exosome release by extracellular DNA is conserved across multiple cell types Authors Iliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R. Journal name FEBS J Publication year 2018 Sample T cells Sample name Jurkat cells - ODN 2395PS stimulated Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
5 Experiment ID 804 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 29953723    Organism Homo sapiens Experiment description Stimulation of exosome release by extracellular DNA is conserved across multiple cell types Authors Iliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R. Journal name FEBS J Publication year 2018 Sample T cells Sample name Jurkat cells - S. salar genomic DNA stimulated Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
6 Experiment ID 805 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 29953723    Organism Homo sapiens Experiment description Stimulation of exosome release by extracellular DNA is conserved across multiple cell types Authors Iliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R. Journal name FEBS J Publication year 2018 Sample T cells Sample name Jurkat cells - PD184352 treated Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
7 Experiment ID 806 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 29953723    Organism Homo sapiens Experiment description Stimulation of exosome release by extracellular DNA is conserved across multiple cell types Authors Iliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R. Journal name FEBS J Publication year 2018 Sample T cells Sample name Jurkat cells - PD184352 treated with S. salar genomic DNA stimulation Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
8 Experiment ID 807 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 29953723    Organism Homo sapiens Experiment description Stimulation of exosome release by extracellular DNA is conserved across multiple cell types Authors Iliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R. Journal name FEBS J Publication year 2018 Sample T cells Sample name Jurkat cells - Heparin treated Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
9 Experiment ID 808 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 29953723    Organism Homo sapiens Experiment description Stimulation of exosome release by extracellular DNA is conserved across multiple cell types Authors Iliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R. Journal name FEBS J Publication year 2018 Sample T cells Sample name Jurkat cells - PD184352 and Heparin treated Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -