Gene description for Pygb
Gene name	brain glycogen phosphorylase
Gene symbol	Pygb
Other names/aliases	-
Species	Mus musculus
Experiment description of studies that identified Pygb in adipocytes
1 Experiment ID 1098 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 24513287    Organism Mus musculus Experiment description Lipid synthesis is promoted by hypoxic adipocyte-derived exosomes in 3T3-L1 cells. Authors Sano S, Izumi Y, Yamaguchi T, Yamazaki T, Tanaka M, Shiota M, Osada-Oka M, Nakamura Y, Wei M, Wanibuchi H, Iwao H, Yoshiyama M. Journal name Biochem Biophys Res Commun Publication year 2014 Sample adipocytes Sample name 3T3-L1- Hypoxic conditions Isolation/purification methods Differential centrifugation Ultracentrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV140281: EV-METRIC:0%, 22%
2 Experiment ID 2366 Identified molecule Protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 36759608    Organism Mus musculus Experiment description Adipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo Authors Kulaj K, Harger A, Bauer M, Caliskan ÖS, Gupta TK, Chiang DM, Milbank E, Reber J, Karlas A, Kotzbeck P, Sailer DN, Volta F, Lutter D, Prakash S, Merl-Pham J, Ntziachristos V, Hauner H, Pfaffl MW, Tschöp MH, Müller TD, Hauck SM, Engel BD, Gerdes JM, Pfluger PT, Krahmer N, Stemmer K. Journal name Nat Commun Publication year 2023 Sample Adipocytes Sample name C57BL/6 J eWAT-derived adipocytes - Lean Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Mass spectrometry RT-PCR EV-TRACK -
3 Experiment ID 2367 Identified molecule Protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 36759608    Organism Mus musculus Experiment description Adipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo Authors Kulaj K, Harger A, Bauer M, Caliskan ÖS, Gupta TK, Chiang DM, Milbank E, Reber J, Karlas A, Kotzbeck P, Sailer DN, Volta F, Lutter D, Prakash S, Merl-Pham J, Ntziachristos V, Hauner H, Pfaffl MW, Tschöp MH, Müller TD, Hauck SM, Engel BD, Gerdes JM, Pfluger PT, Krahmer N, Stemmer K. Journal name Nat Commun Publication year 2023 Sample Adipocytes Sample name C57BL/6 J eWAT-derived adipocytes - Lean Isolation/purification methods Centrifugal ultrafiltration Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK -
4 Experiment ID 2368 Identified molecule Protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 36759608    Organism Mus musculus Experiment description Adipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo Authors Kulaj K, Harger A, Bauer M, Caliskan ÖS, Gupta TK, Chiang DM, Milbank E, Reber J, Karlas A, Kotzbeck P, Sailer DN, Volta F, Lutter D, Prakash S, Merl-Pham J, Ntziachristos V, Hauner H, Pfaffl MW, Tschöp MH, Müller TD, Hauck SM, Engel BD, Gerdes JM, Pfluger PT, Krahmer N, Stemmer K. Journal name Nat Commun Publication year 2023 Sample Adipocytes Sample name C57BL/6 J eWAT-derived adipocytes - Diet-induced obesity Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Mass spectrometry RT-PCR EV-TRACK -
5 Experiment ID 2369 Identified molecule Protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 36759608    Organism Mus musculus Experiment description Adipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo Authors Kulaj K, Harger A, Bauer M, Caliskan ÖS, Gupta TK, Chiang DM, Milbank E, Reber J, Karlas A, Kotzbeck P, Sailer DN, Volta F, Lutter D, Prakash S, Merl-Pham J, Ntziachristos V, Hauner H, Pfaffl MW, Tschöp MH, Müller TD, Hauck SM, Engel BD, Gerdes JM, Pfluger PT, Krahmer N, Stemmer K. Journal name Nat Commun Publication year 2023 Sample Adipocytes Sample name C57BL/6 J eWAT-derived adipocytes - Diet-induced obesity Isolation/purification methods Centrifugal ultrafiltration Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK -