Gene description for Actb
Gene name actin, beta
Gene symbol Actb
Other names/aliases Actx
E430023M04Rik
beta-actin
Species Mus musculus
 Experiment description of studies that identified Actb in Fibroblasts

1
Experiment ID489
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23260141   
OrganismMus musculus
Experiment descriptionExosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
AuthorsLuga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year2012
SampleFibroblasts
Sample nameNormal-Fibroblasts (L cells)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120034: EV-METRIC:44%, 38%

2
Experiment ID1572
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33785738   
OrganismMus musculus
Experiment descriptionSelective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs
AuthorsTodkar K, Chikhi L, Desjardins V, El-Mortada F, Pépin G, Germain M.
Journal name Nat Commun
Publication year2021
SampleFibroblasts
Sample nameMEF
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
qPCR
EV-TRACK -

3
Experiment ID1575
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33785738   
OrganismMus musculus
Experiment descriptionSelective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs
AuthorsTodkar K, Chikhi L, Desjardins V, El-Mortada F, Pépin G, Germain M.
Journal name Nat Commun
Publication year2021
SampleFibroblasts
Sample nameMEF-derived EVs treated with Trypsin
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

4
Experiment ID1576
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33785738   
OrganismMus musculus
Experiment descriptionSelective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs
AuthorsTodkar K, Chikhi L, Desjardins V, El-Mortada F, Pépin G, Germain M.
Journal name Nat Commun
Publication year2021
SampleFibroblasts
Sample nameMEF-derived EVs treated with Trypsin and Triton X-100
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

5
Experiment ID1577
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33785738   
OrganismMus musculus
Experiment descriptionSelective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs
AuthorsTodkar K, Chikhi L, Desjardins V, El-Mortada F, Pépin G, Germain M.
Journal name Nat Commun
Publication year2021
SampleFibroblasts
Sample nameMEF
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

6
Experiment ID1578
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33785738   
OrganismMus musculus
Experiment descriptionSelective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs
AuthorsTodkar K, Chikhi L, Desjardins V, El-Mortada F, Pépin G, Germain M.
Journal name Nat Commun
Publication year2021
SampleFibroblasts
Sample nameMEF
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

7
Experiment ID1579
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33785738   
OrganismMus musculus
Experiment descriptionSelective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs
AuthorsTodkar K, Chikhi L, Desjardins V, El-Mortada F, Pépin G, Germain M.
Journal name Nat Commun
Publication year2021
SampleFibroblasts
Sample nameMEF
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProteinmRNA
Methods used in the studyWestern blotting
qPCR
EV-TRACK -

8
Experiment ID1580
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33785738   
OrganismMus musculus
Experiment descriptionSelective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs
AuthorsTodkar K, Chikhi L, Desjardins V, El-Mortada F, Pépin G, Germain M.
Journal name Nat Commun
Publication year2021
SampleFibroblasts
Sample nameMEF
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

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