| Experiment ID | 349 |
| Identified molecule | protein
|
| Extracellular vesicle type | Exosomes |
| MISEV | EVs |
| Identification method | Mass spectrometry [Orbitrap]
|
| PubMed ID |
22285593
|
| Organism | Homo sapiens |
| Experiment description | Comparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes. |
| Authors | Tauro BJ, Greening DW, Mathias RA, Ji H, Mathivanan S, Scott AM, Simpson RJ |
| Journal name |
Methods
|
| Publication year | 2012 |
| Sample | Colorectal cancer cells |
| Sample name | LIM1863 - OptiPrep density gradient |
| Isolation/purification methods | Differential centrifugation Filtration Ultracentrifugation Density gradient centrifugation |
| Flotation density | - |
| Molecules identified in the study | Protein |
| Methods used in the study | Mass spectrometry [Orbitrap] Western blotting |
| EV-TRACK |
EV120024: EV-METRIC:25%, 33%, 50%
|