Gene description for Flot1
Gene name	flotillin 1
Gene symbol	Flot1
Other names/aliases	reggie-2
Species	Mus musculus
Experiment description of studies that identified Flot1 in Primary cortical neurons
1 Experiment ID 1629 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 29348617    Organism Mus musculus Experiment description Neuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures Authors Miranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G. Journal name Nat Commun Publication year 2018 Sample Primary cortical neurons Sample name C57BL/6 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Lipid Methods used in the study Western blotting LC-MS EV-TRACK -
2 Experiment ID 1630 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 29348617    Organism Mus musculus Experiment description Neuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures Authors Miranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G. Journal name Nat Commun Publication year 2018 Sample Primary cortical neurons Sample name C57BL/6 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Lipid Methods used in the study Western blotting LC-MS EV-TRACK -
3 Experiment ID 1640 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 29348617    Organism Mus musculus Experiment description Neuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures Authors Miranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G. Journal name Nat Commun Publication year 2018 Sample Primary cortical neurons Sample name C57BL/6 - Fraction 4 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
4 Experiment ID 1641 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 29348617    Organism Mus musculus Experiment description Neuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures Authors Miranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G. Journal name Nat Commun Publication year 2018 Sample Primary cortical neurons Sample name C57BL/6 - Fraction 5 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
5 Experiment ID 1642 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 29348617    Organism Mus musculus Experiment description Neuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures Authors Miranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G. Journal name Nat Commun Publication year 2018 Sample Primary cortical neurons Sample name C57BL/6 - Fraction 6 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
6 Experiment ID 1643 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 29348617    Organism Mus musculus Experiment description Neuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures Authors Miranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G. Journal name Nat Commun Publication year 2018 Sample Primary cortical neurons Sample name C57BL/6 - Fraction 7 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
7 Experiment ID 1644 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 29348617    Organism Mus musculus Experiment description Neuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures Authors Miranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G. Journal name Nat Commun Publication year 2018 Sample Primary cortical neurons Sample name C57BL/6 - Fraction 8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
8 Experiment ID 1787 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 36594832    Organism Mus musculus Experiment description Dynamic release of neuronal extracellular vesicles containing miR-21a-5p is induced by hypoxia Authors Korvenlaita N, Gómez-Budia M, Scoyni F, Pistono C, Giudice L, Eamen S, Loppi S, de Sande AH, Huremagic B, Bouvy-Liivrand M, Heinäniemi M, Kaikkonen MU, Cheng L, Hill AF, Kanninen KM, Jenster GW, van Royen ME, Ramiro L, Montaner J, Batkova T, Mikulik R, Giugno R, Jolkkonen J, Korhonen P, Malm T. Journal name J Extracell Vesicles Publication year 2023 Sample Primary cortical neurons Sample name CXN - C57Bl/6J mice embryos Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting qPCR EV-TRACK -