Gene description for Hspa8
Gene name	heat shock protein 8
Gene symbol	Hspa8
Other names/aliases	2410008N15Rik Hsc70 Hsc71 Hsc73 Hsp73 Hspa10
Species	Mus musculus
Experiment description of studies that identified Hspa8 in Mast cells
1 Experiment ID 7 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 12626558    Organism Mus musculus Experiment description Mast cell-derived exosomes induce phenotypic and functional maturation of dendritic cells and elicit specific immune responses in vivo. Authors Skokos D, Botros HG, Demeure C, Morin J, Peronet R, Birkenmeier G, Boudaly S, Mecheri S. Journal name JIMMU Publication year 2003 Sample Mast cells Sample name Bone marrow-derived mast cells Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
2 Experiment ID 15 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 17486113    Organism Mus musculus Homo sapiens Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Authors Valadi H, Ekströ, Bossios A, Sjöand M, Lee JJ, Lotvall JO Journal name NCB Publication year 2007 Sample Mast cells Sample name MC9 Bone marrow-derived mast cells HMC-1 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.11-1.21 g/mL Molecules identified in the study Protein mRNA miRNA Methods used in the study Mass spectrometry [MALDI TOF] Western blotting Microarray MicroRNA array [miRCURY LNA Array] EV-TRACK -
3 Experiment ID 424 Identified molecule mRNA Extracellular vesicle type Exosomes/Nanovesicles MISEV EVs Identification method Microarray PubMed ID 21179422    Organism Mus musculus Experiment description Exosomes communicate protective messages during oxidative stress; possible role of exosomal shuttle RNA. Authors Eldh M, Ekström K, Valadi H, Sjöstrand M, Olsson B, Jernås M, Lötvall J. Journal name PLoS One Publication year 2010 Sample Mast cells Sample name Mast cells (MC/9) Isolation/purification methods - Flotation density - Molecules identified in the study mRNA Methods used in the study Microarray EV-TRACK EV100074: EV-METRIC:14%
4 Experiment ID 425 Identified molecule mRNA Extracellular vesicle type Exosomes/Nanovesicles MISEV EVs Identification method Microarray PubMed ID 21179422    Organism Mus musculus Experiment description Exosomes communicate protective messages during oxidative stress; possible role of exosomal shuttle RNA. Authors Eldh M, Ekström K, Valadi H, Sjöstrand M, Olsson B, Jernås M, Lötvall J. Journal name PLoS One Publication year 2010 Sample Mast cells Sample name Oxidatively stressed-Mast cells (MC/9) Isolation/purification methods - Flotation density - Molecules identified in the study mRNA Methods used in the study Microarray EV-TRACK EV100074: EV-METRIC:14%