Gene description for Mdh2
Gene name malate dehydrogenase 2, NAD (mitochondrial)
Gene symbol Mdh2
Other names/aliases MDH
Mdh-2
Mor-1
Mor1
Species Mus musculus
 Experiment description of studies that identified Mdh2 in Adipocytes

1
Experiment ID304
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
MISEVEVs
Identification method Mass spectrometry [LCQ-DECA XP]
PubMed ID 17478559   
OrganismMus musculus
Experiment descriptionIdentification and characterization of microvesicles secreted by 3T3-L1 adipocytes: redox- and hormone-dependent induction of milk fat globule-epidermal growth 8-associated microvesicles
AuthorsAoki N, Jin-no S, Nakagawa Y, Asai N, Arakawa E, Tamura N, Tamura T, Matsuda T
Journal name Endocrinology
Publication year2007
SampleAdipocytes
Sample name3T3-L1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.13–-1.20 g/mL
Molecules identified in the studyProtein
Methods used in the studyImmunoelectron microscopy
Western blotting
Mass spectrometry [LCQ-DECA XP]
EV-TRACK -

2
Experiment ID1098
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 24513287   
OrganismMus musculus
Experiment descriptionLipid synthesis is promoted by hypoxic adipocyte-derived exosomes in 3T3-L1 cells.
AuthorsSano S, Izumi Y, Yamaguchi T, Yamazaki T, Tanaka M, Shiota M, Osada-Oka M, Nakamura Y, Wei M, Wanibuchi H, Iwao H, Yoshiyama M.
Journal name Biochem Biophys Res Commun
Publication year2014
Sampleadipocytes
Sample name3T3-L1- Hypoxic conditions
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140281: EV-METRIC:0%, 22%

3
Experiment ID2366
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 36759608   
OrganismMus musculus
Experiment descriptionAdipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo
AuthorsKulaj K, Harger A, Bauer M, Caliskan ÖS, Gupta TK, Chiang DM, Milbank E, Reber J, Karlas A, Kotzbeck P, Sailer DN, Volta F, Lutter D, Prakash S, Merl-Pham J, Ntziachristos V, Hauner H, Pfaffl MW, Tschöp MH, Müller TD, Hauck SM, Engel BD, Gerdes JM, Pfluger PT, Krahmer N, Stemmer K.
Journal name Nat Commun
Publication year2023
SampleAdipocytes
Sample nameC57BL/6 J eWAT-derived adipocytes - Lean
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyMass spectrometry
RT-PCR
EV-TRACK -

4
Experiment ID2367
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 36759608   
OrganismMus musculus
Experiment descriptionAdipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo
AuthorsKulaj K, Harger A, Bauer M, Caliskan ÖS, Gupta TK, Chiang DM, Milbank E, Reber J, Karlas A, Kotzbeck P, Sailer DN, Volta F, Lutter D, Prakash S, Merl-Pham J, Ntziachristos V, Hauner H, Pfaffl MW, Tschöp MH, Müller TD, Hauck SM, Engel BD, Gerdes JM, Pfluger PT, Krahmer N, Stemmer K.
Journal name Nat Commun
Publication year2023
SampleAdipocytes
Sample nameC57BL/6 J eWAT-derived adipocytes - Lean
Isolation/purification methodsCentrifugal ultrafiltration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

5
Experiment ID2368
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 36759608   
OrganismMus musculus
Experiment descriptionAdipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo
AuthorsKulaj K, Harger A, Bauer M, Caliskan ÖS, Gupta TK, Chiang DM, Milbank E, Reber J, Karlas A, Kotzbeck P, Sailer DN, Volta F, Lutter D, Prakash S, Merl-Pham J, Ntziachristos V, Hauner H, Pfaffl MW, Tschöp MH, Müller TD, Hauck SM, Engel BD, Gerdes JM, Pfluger PT, Krahmer N, Stemmer K.
Journal name Nat Commun
Publication year2023
SampleAdipocytes
Sample nameC57BL/6 J eWAT-derived adipocytes - Diet-induced obesity
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyMass spectrometry
RT-PCR
EV-TRACK -

6
Experiment ID2369
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 36759608   
OrganismMus musculus
Experiment descriptionAdipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo
AuthorsKulaj K, Harger A, Bauer M, Caliskan ÖS, Gupta TK, Chiang DM, Milbank E, Reber J, Karlas A, Kotzbeck P, Sailer DN, Volta F, Lutter D, Prakash S, Merl-Pham J, Ntziachristos V, Hauner H, Pfaffl MW, Tschöp MH, Müller TD, Hauck SM, Engel BD, Gerdes JM, Pfluger PT, Krahmer N, Stemmer K.
Journal name Nat Commun
Publication year2023
SampleAdipocytes
Sample nameC57BL/6 J eWAT-derived adipocytes - Diet-induced obesity
Isolation/purification methodsCentrifugal ultrafiltration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

7
Experiment ID2370
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 36759608   
OrganismHomo sapiens
Experiment descriptionAdipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo
AuthorsKulaj K, Harger A, Bauer M, Caliskan ÖS, Gupta TK, Chiang DM, Milbank E, Reber J, Karlas A, Kotzbeck P, Sailer DN, Volta F, Lutter D, Prakash S, Merl-Pham J, Ntziachristos V, Hauner H, Pfaffl MW, Tschöp MH, Müller TD, Hauck SM, Engel BD, Gerdes JM, Pfluger PT, Krahmer N, Stemmer K.
Journal name Nat Commun
Publication year2023
SampleAdipocytes
Sample nameLiposuction patient-derived adipocytes
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

<