Gene description for Hspa1a
Gene name heat shock protein 1A
Gene symbol Hspa1a
Other names/aliases Hsp70-3
Hsp70.3
Hsp72
hsp68
hsp70A1
Species Mus musculus
 Experiment description of studies that identified Hspa1a in Fibroblasts

1
Experiment ID1572
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33785738   
OrganismMus musculus
Experiment descriptionSelective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs
AuthorsTodkar K, Chikhi L, Desjardins V, El-Mortada F, Pépin G, Germain M.
Journal name Nat Commun
Publication year2021
SampleFibroblasts
Sample nameMEF
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
qPCR
EV-TRACK -

2
Experiment ID1575
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33785738   
OrganismMus musculus
Experiment descriptionSelective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs
AuthorsTodkar K, Chikhi L, Desjardins V, El-Mortada F, Pépin G, Germain M.
Journal name Nat Commun
Publication year2021
SampleFibroblasts
Sample nameMEF-derived EVs treated with Trypsin
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

3
Experiment ID1576
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33785738   
OrganismMus musculus
Experiment descriptionSelective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs
AuthorsTodkar K, Chikhi L, Desjardins V, El-Mortada F, Pépin G, Germain M.
Journal name Nat Commun
Publication year2021
SampleFibroblasts
Sample nameMEF-derived EVs treated with Trypsin and Triton X-100
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

4
Experiment ID1577
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33785738   
OrganismMus musculus
Experiment descriptionSelective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs
AuthorsTodkar K, Chikhi L, Desjardins V, El-Mortada F, Pépin G, Germain M.
Journal name Nat Commun
Publication year2021
SampleFibroblasts
Sample nameMEF
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

5
Experiment ID1580
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33785738   
OrganismMus musculus
Experiment descriptionSelective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs
AuthorsTodkar K, Chikhi L, Desjardins V, El-Mortada F, Pépin G, Germain M.
Journal name Nat Commun
Publication year2021
SampleFibroblasts
Sample nameMEF
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

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