Gene description for TMED4
Gene name	transmembrane p24 trafficking protein 4
Gene symbol	TMED4
Other names/aliases	ERS25 GMP25iso HNLF p24a3 p24alpha3
Species	Homo sapiens
Experiment description of studies that identified TMED4 in Colorectal cancer cells
1 Experiment ID 303 Identified molecule mrna Extracellular vesicle type Microvesicles MISEV EVs Identification method Microarray [Illumina] PubMed ID 19930720    Organism Homo sapiens Experiment description Colorectal cancer cell-derived microvesicles are enriched in cell cycle-related mRNAs that promote proliferation of endothelial cells Authors Hong BS, Cho JH, Kim H, Choi EJ, Rho S, Kim J, Kim JH, Choi DS, Kim YK, Hwang D, Gho YS Journal name BMC Genomics Publication year 2009 Sample Colorectal cancer cells Sample name SW480 Isolation/purification methods Differential centrifugation Ultrafiltration OptiPrep density gradient Flotation density 1.09 g/mL Molecules identified in the study Protein mRNA Methods used in the study Western blotting RT-PCR Microarray [Illumina] EV-TRACK -
2 Experiment ID 480 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 23161513    Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130004: EV-METRIC:44%
3 Experiment ID 481 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 23161513    Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130004: EV-METRIC:44%
4 Experiment ID 482 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 23161513    Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130004: EV-METRIC:44%
5 Experiment ID 587 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Colorectal cancer cells Sample name Colo205 Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
6 Experiment ID 591 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Colorectal cancer cells Sample name HT29 Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
7 Experiment ID 696 Identified molecule protein Extracellular vesicle type Microvesicles MISEV EVs Identification method Mass spectrometry PubMed ID 28842968    Organism Homo sapiens Experiment description Insulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles. Authors Liem M, Ang CS, Mathivanan S. Journal name Proteomics Publication year 2017 Sample Colorectal cancer cells Sample name LIM1215- insulin induced Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV170021: EV-METRIC:44%, 44%
8 Experiment ID 697 Identified molecule protein Extracellular vesicle type Microvesicles MISEV EVs Identification method Mass spectrometry PubMed ID 28842968    Organism Homo sapiens Experiment description Insulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles. Authors Liem M, Ang CS, Mathivanan S. Journal name Proteomics Publication year 2017 Sample Colorectal cancer cells Sample name LIM1215- non-induced Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV170021: EV-METRIC:44%, 44%
9 Experiment ID 1049 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 28842968    Organism Homo sapiens Experiment description Insulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles. Authors Liem M, Ang CS, Mathivanan S. Journal name Proteomics. Publication year 2017 Sample Colorectal cancer cells Sample name LIM1215 - NI Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV170021: EV-METRIC:44%, 44%
10 Experiment ID 1050 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 28842968    Organism Homo sapiens Experiment description Insulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles. Authors Liem M, Ang CS, Mathivanan S. Journal name Proteomics. Publication year 2017 Sample Colorectal cancer cells Sample name lim1215 - II Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV170021: EV-METRIC:44%, 44%