Gene description for PUF60
Gene name poly(U) binding splicing factor 60
Gene symbol PUF60
Other names/aliases FIR
RoBPI
SIAHBP1
VRJS
Species Homo sapiens
 Experiment description of studies that identified PUF60 in Gastric cancer cells

1
Experiment ID1493
Identified moleculeProtein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 33991177   
OrganismHomo sapiens
Experiment descriptionCancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
AuthorsCarli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year2021
SampleGastric cancer cells
Sample nameMKN1 - 100K pellet
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

2
Experiment ID1494
Identified moleculeProtein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 33991177   
OrganismHomo sapiens
Experiment descriptionCancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
AuthorsCarli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year2021
SampleGastric cancer cells
Sample nameMKN1 - 100K pellet
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

3
Experiment ID1495
Identified moleculeProtein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 33991177   
OrganismHomo sapiens
Experiment descriptionCancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
AuthorsCarli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year2021
SampleGastric cancer cells
Sample nameMKN1 - 100K pellet
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

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