Gene description for Pgap1
Gene name post-GPI attachment to proteins 1
Gene symbol Pgap1
Other names/aliases 5033403E17Rik
9030223K07Rik
A530084K22
D230012E17Rik
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Species Mus musculus
 Experiment description of studies that identified Pgap1 in Adipocytes

1
Experiment ID2366
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 36759608   
OrganismMus musculus
Experiment descriptionAdipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo
AuthorsKulaj K, Harger A, Bauer M, Caliskan ÖS, Gupta TK, Chiang DM, Milbank E, Reber J, Karlas A, Kotzbeck P, Sailer DN, Volta F, Lutter D, Prakash S, Merl-Pham J, Ntziachristos V, Hauner H, Pfaffl MW, Tschöp MH, Müller TD, Hauck SM, Engel BD, Gerdes JM, Pfluger PT, Krahmer N, Stemmer K.
Journal name Nat Commun
Publication year2023
SampleAdipocytes
Sample nameC57BL/6 J eWAT-derived adipocytes - Lean
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyMass spectrometry
RT-PCR
EV-TRACK -

2
Experiment ID2367
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 36759608   
OrganismMus musculus
Experiment descriptionAdipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo
AuthorsKulaj K, Harger A, Bauer M, Caliskan ÖS, Gupta TK, Chiang DM, Milbank E, Reber J, Karlas A, Kotzbeck P, Sailer DN, Volta F, Lutter D, Prakash S, Merl-Pham J, Ntziachristos V, Hauner H, Pfaffl MW, Tschöp MH, Müller TD, Hauck SM, Engel BD, Gerdes JM, Pfluger PT, Krahmer N, Stemmer K.
Journal name Nat Commun
Publication year2023
SampleAdipocytes
Sample nameC57BL/6 J eWAT-derived adipocytes - Lean
Isolation/purification methodsCentrifugal ultrafiltration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

3
Experiment ID2368
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 36759608   
OrganismMus musculus
Experiment descriptionAdipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo
AuthorsKulaj K, Harger A, Bauer M, Caliskan ÖS, Gupta TK, Chiang DM, Milbank E, Reber J, Karlas A, Kotzbeck P, Sailer DN, Volta F, Lutter D, Prakash S, Merl-Pham J, Ntziachristos V, Hauner H, Pfaffl MW, Tschöp MH, Müller TD, Hauck SM, Engel BD, Gerdes JM, Pfluger PT, Krahmer N, Stemmer K.
Journal name Nat Commun
Publication year2023
SampleAdipocytes
Sample nameC57BL/6 J eWAT-derived adipocytes - Diet-induced obesity
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyMass spectrometry
RT-PCR
EV-TRACK -

4
Experiment ID2369
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 36759608   
OrganismMus musculus
Experiment descriptionAdipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo
AuthorsKulaj K, Harger A, Bauer M, Caliskan ÖS, Gupta TK, Chiang DM, Milbank E, Reber J, Karlas A, Kotzbeck P, Sailer DN, Volta F, Lutter D, Prakash S, Merl-Pham J, Ntziachristos V, Hauner H, Pfaffl MW, Tschöp MH, Müller TD, Hauck SM, Engel BD, Gerdes JM, Pfluger PT, Krahmer N, Stemmer K.
Journal name Nat Commun
Publication year2023
SampleAdipocytes
Sample nameC57BL/6 J eWAT-derived adipocytes - Diet-induced obesity
Isolation/purification methodsCentrifugal ultrafiltration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

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