Gene description for Gapdh
Gene name glyceraldehyde-3-phosphate dehydrogenase
Gene symbol Gapdh
Other names/aliases BARS-38
Gapd
Species Rattus norvegicus
 Experiment description of studies that identified Gapdh in Cardiomyocytes

1
Experiment ID831
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
Western blotting
PubMed ID 23376832   
OrganismRattus norvegicus
Experiment descriptionCardiac myocyte exosomes: stability, HSP60, and proteomics
AuthorsMalik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA.
Journal name Am J Physiol Heart Circ Physiol
Publication year2013
SampleCardiomyocytes
Sample nameCardiomyocyte cells - exosomes exposed to 65.1 mM ethanol - Rep 1
Isolation/purification methodsDifferential centrifugation
Filtration
ExoQuick
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV130100: EV-METRIC:38%, 13%

2
Experiment ID811
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 23376832   
OrganismRattus norvegicus
Experiment descriptionCardiac myocyte exosomes: stability, HSP60, and proteomics
AuthorsMalik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA.
Journal name Am J Physiol Heart Circ Physiol
Publication year2013
SampleCardiomyocytes
Sample nameCardiomyocyte cells - exosomes incubated at 4°C
Isolation/purification methodsDifferential centrifugation
Filtration
ExoQuick
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK EV130100: EV-METRIC:38%, 13%

3
Experiment ID812
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 23376832   
OrganismRattus norvegicus
Experiment descriptionCardiac myocyte exosomes: stability, HSP60, and proteomics
AuthorsMalik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA.
Journal name Am J Physiol Heart Circ Physiol
Publication year2013
SampleCardiomyocytes
Sample nameCardiomyocyte cells - exosomes incubated at 25°C
Isolation/purification methodsDifferential centrifugation
Filtration
ExoQuick
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK EV130100: EV-METRIC:38%, 13%

4
Experiment ID813
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 23376832   
OrganismRattus norvegicus
Experiment descriptionCardiac myocyte exosomes: stability, HSP60, and proteomics
AuthorsMalik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA.
Journal name Am J Physiol Heart Circ Physiol
Publication year2013
SampleCardiomyocytes
Sample nameCardiomyocyte cells - exosomes incubated at 37°C
Isolation/purification methodsDifferential centrifugation
Filtration
ExoQuick
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK EV130100: EV-METRIC:38%, 13%

5
Experiment ID814
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 23376832   
OrganismRattus norvegicus
Experiment descriptionCardiac myocyte exosomes: stability, HSP60, and proteomics
AuthorsMalik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA.
Journal name Am J Physiol Heart Circ Physiol
Publication year2013
SampleCardiomyocytes
Sample nameCardiomyocyte cells - exosomes incubated at 42°C
Isolation/purification methodsDifferential centrifugation
Filtration
ExoQuick
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK EV130100: EV-METRIC:38%, 13%

6
Experiment ID832
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
Mass spectrometry
PubMed ID 23376832   
OrganismRattus norvegicus
Experiment descriptionCardiac myocyte exosomes: stability, HSP60, and proteomics
AuthorsMalik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA.
Journal name Am J Physiol Heart Circ Physiol
Publication year2013
SampleCardiomyocytes
Sample nameCardiomyocyte cells - exosomes exposed to 65.1 mM ethanol - Rep 2
Isolation/purification methodsDifferential centrifugation
Filtration
ExoQuick
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV130100: EV-METRIC:38%, 13%

7
Experiment ID833
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
Mass spectrometry
PubMed ID 23376832   
OrganismRattus norvegicus
Experiment descriptionCardiac myocyte exosomes: stability, HSP60, and proteomics
AuthorsMalik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA.
Journal name Am J Physiol Heart Circ Physiol
Publication year2013
SampleCardiomyocytes
Sample nameCardiomyocyte cells - exosomes exposed to 65.1 mM ethanol - Rep 3
Isolation/purification methodsDifferential centrifugation
Filtration
ExoQuick
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV130100: EV-METRIC:38%, 13%

8
Experiment ID835
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
Mass spectrometry
PubMed ID 23376832   
OrganismRattus norvegicus
Experiment descriptionCardiac myocyte exosomes: stability, HSP60, and proteomics
AuthorsMalik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA.
Journal name Am J Physiol Heart Circ Physiol
Publication year2013
SampleCardiomyocytes
Sample nameCardiomyocyte cells - mild hypoxia - Rep 1
Isolation/purification methodsDifferential centrifugation
Filtration
ExoQuick
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV130100: EV-METRIC:38%, 13%

9
Experiment ID836
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
Mass spectrometry
PubMed ID 23376832   
OrganismRattus norvegicus
Experiment descriptionCardiac myocyte exosomes: stability, HSP60, and proteomics
AuthorsMalik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA.
Journal name Am J Physiol Heart Circ Physiol
Publication year2013
SampleCardiomyocytes
Sample nameCardiomyocyte cells - mild hypoxia - Rep 2
Isolation/purification methodsDifferential centrifugation
Filtration
ExoQuick
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV130100: EV-METRIC:38%, 13%

10
Experiment ID837
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
Mass spectrometry
PubMed ID 23376832   
OrganismRattus norvegicus
Experiment descriptionCardiac myocyte exosomes: stability, HSP60, and proteomics
AuthorsMalik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA.
Journal name Am J Physiol Heart Circ Physiol
Publication year2013
SampleCardiomyocytes
Sample nameCardiomyocyte cells - mild hypoxia - Rep 3
Isolation/purification methodsDifferential centrifugation
Filtration
ExoQuick
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV130100: EV-METRIC:38%, 13%

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