Gene description for Hspa4
Gene name	heat shock protein family A (Hsp70) member 4
Gene symbol	Hspa4
Other names/aliases	Hsp110 Hsp70 irp94
Species	Rattus norvegicus
Experiment description of studies that identified Hspa4 in Reticulocytes
1 Experiment ID 90 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry [Orbitrap] Western blotting PubMed ID 21828046    Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D2 Rep 1 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV110005: EV-METRIC:44%
2 Experiment ID 94 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry [Orbitrap] Western blotting PubMed ID 21828046    Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D4 Rep 1 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV110005: EV-METRIC:44%
3 Experiment ID 95 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry [Orbitrap] Western blotting PubMed ID 21828046    Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D7 Rep 1 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.13-1.25 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV110005: EV-METRIC:44%
4 Experiment ID 96 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046    Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D2 Rep 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV110005: EV-METRIC:44%
5 Experiment ID 97 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046    Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D4 Rep 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV110005: EV-METRIC:44%
6 Experiment ID 98 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046    Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D7 Rep 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.13-1.25 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV110005: EV-METRIC:44%