Gene description for Wnt3a
Gene name Wnt family member 3A
Gene symbol Wnt3a
Other names/aliases -
Species Rattus norvegicus
 Experiment description of studies that identified Wnt3a in Primary microglial cells

1
Experiment ID1145
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23173708   
OrganismRattus norvegicus
Experiment descriptionWnt3a induces exosome secretion from primary cultured rat microglia.
AuthorsHooper C, Sainz-Fuertes R, Lynham S, Hye A, Killick R, Warley A, Bolondi C, Pocock J, Lovestone S.
Journal name BMC Neurosci
Publication year2012
SamplePrimary microglial cells
Sample nameWnt3a stimulated (8 hour)
100,000g fraction
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120065: EV-METRIC:22%, 22%

2
Experiment ID1146
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 23173708   
OrganismRattus norvegicus
Experiment descriptionWnt3a induces exosome secretion from primary cultured rat microglia.
AuthorsHooper C, Sainz-Fuertes R, Lynham S, Hye A, Killick R, Warley A, Bolondi C, Pocock J, Lovestone S.
Journal name BMC Neurosci
Publication year2012
SamplePrimary microglial cells
Sample nameWnt3a stimulated (8 hour)
100,000g fraction
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK EV120065: EV-METRIC:22%, 22%

3
Experiment ID1147
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 23173708   
OrganismRattus norvegicus
Experiment descriptionWnt3a induces exosome secretion from primary cultured rat microglia.
AuthorsHooper C, Sainz-Fuertes R, Lynham S, Hye A, Killick R, Warley A, Bolondi C, Pocock J, Lovestone S.
Journal name BMC Neurosci
Publication year2012
SamplePrimary microglial cells
Sample nameWnt3a stimulated (36 hour)
100,000g fraction
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK EV120065: EV-METRIC:22%, 22%

4
Experiment ID1148
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 23173708   
OrganismRattus norvegicus
Experiment descriptionWnt3a induces exosome secretion from primary cultured rat microglia.
AuthorsHooper C, Sainz-Fuertes R, Lynham S, Hye A, Killick R, Warley A, Bolondi C, Pocock J, Lovestone S.
Journal name BMC Neurosci
Publication year2012
SamplePrimary microglial cells
Sample nameWnt3a stimulated (36 hour)
200,000g fraction
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK EV120065: EV-METRIC:22%, 22%

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