Gene description for HSPA1A
Gene name	heat shock protein family A (Hsp70) member 1A
Gene symbol	HSPA1A
Other names/aliases	HEL-S-103 HSP70 HSP70-1 HSP70-1A HSP70-2 HSP70.1 HSP70.2 HSP70I HSP72 HSPA1
Species	Homo sapiens
Experiment description of studies that identified HSPA1A in Breast cancer cells
1 Experiment ID 886 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 25102470    Organism Homo sapiens Experiment description Examination of the specificity of tumor cell derived exosomes with tumor cells in vitro Authors Smyth TJ, Redzic JS, Graner MW, Anchordoquy TJ. Journal name Biochim Biophys Acta Publication year 2014 Sample Breast cancer cells Sample name MCF-7 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.10-1.20 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV140013: EV-METRIC:56%
2 Experiment ID 1029 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 26378940    Organism Homo sapiens Experiment description Redefining the Breast Cancer Exosome Proteome by Tandem Mass Tag Quantitative Proteomics and Multivariate Cluster Analysis. Authors Clark DJ, Fondrie WE, Liao Z, Hanson PI, Fulton A, Mao L, Yang AJ. Journal name Anal Chem. Publication year 2015 Sample Breast cancer cells Sample name SKBR3B - 100 K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV150004: EV-METRIC:67%, 38%
3 Experiment ID 1456 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 31969559    Organism Homo sapiens Experiment description Exosome-transmitted miR-567 reverses trastuzumab resistance by inhibiting ATG5 in breast cancer Authors Han M, Hu J, Lu P, Cao H, Yu C, Li X, Qian X, Yang X, Yang Y, Han N, Dou D, Zhang F, Ye M, Yang C, Gu Y, Dong H. Journal name Cell Death Dis Publication year 2020 Sample Breast cancer cells Sample name SKBR-3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting qRT-PCR EV-TRACK -
4 Experiment ID 1457 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 31969559    Organism Homo sapiens Experiment description Exosome-transmitted miR-567 reverses trastuzumab resistance by inhibiting ATG5 in breast cancer Authors Han M, Hu J, Lu P, Cao H, Yu C, Li X, Qian X, Yang X, Yang Y, Han N, Dou D, Zhang F, Ye M, Yang C, Gu Y, Dong H. Journal name Cell Death Dis Publication year 2020 Sample Breast cancer cells Sample name BT474 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting qRT-PCR EV-TRACK -
5 Experiment ID 1998 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 34112803    Organism Homo sapiens Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution Authors Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. Journal name Nat Commun Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Centrifugal ultrafiltration Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
6 Experiment ID 1999 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 34112803    Organism Homo sapiens Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution Authors Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. Journal name Nat Commun Publication year 2021 Sample Breast cancer cells Sample name MCF7 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Centrifugal ultrafiltration Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
7 Experiment ID 2003 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 34112803    Organism Mus musculus Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution Authors Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. Journal name Nat Commun Publication year 2021 Sample Breast cancer cells Sample name EO771 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Centrifugal ultrafiltration Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
8 Experiment ID 2004 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 34112803    Organism Mus musculus Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution Authors Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. Journal name Nat Commun Publication year 2021 Sample Breast cancer cells Sample name EO771 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Centrifugal ultrafiltration Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
9 Experiment ID 2005 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 34112803    Organism Mus musculus Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution Authors Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. Journal name Nat Commun Publication year 2021 Sample Breast cancer cells Sample name EO771 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Centrifugal ultrafiltration Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
10 Experiment ID 2006 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 34112803    Organism Mus musculus Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution Authors Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. Journal name Nat Commun Publication year 2021 Sample Breast cancer cells Sample name EO771 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Centrifugal ultrafiltration Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
11 Experiment ID 2449 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Western blotting PubMed ID 34465793    Organism Homo sapiens Experiment description Exosomal miR-19a and IBSP cooperate to induce osteolytic bone metastasis of estrogen receptor-positive breast cancer Authors Wu K, Feng J, Lyu F, Xing F, Sharma S, Liu Y, Wu SY, Zhao D, Tyagi A, Deshpande RP, Pei X, Ruiz MG, Takahashi H, Tsuzuki S, Kimura T, Mo YY, Shiozawa Y, Singh R, Watabe K. Journal name Nat Commun Publication year 2021 Sample Breast cancer cells Sample name MCF7BoM2 - 100K pellet Isolation/purification methods Centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting Immuno-EM miRNA array qPCR EV-TRACK -
12 Experiment ID 2867 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 30918259    Organism Homo sapiens Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A Authors Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC. Journal name Nat Commun Publication year 2019 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
13 Experiment ID 2870 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 30918259    Organism Homo sapiens Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A Authors Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC. Journal name Nat Commun Publication year 2019 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
14 Experiment ID 3350 Identified molecule protein Extracellular vesicle type Supermeres MISEV EV-like nanoparticles Identification method Mass spectrometry PubMed ID 34887515    Organism Homo sapiens Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ. Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry ELISA EV-TRACK -