Gene description for HSPA1A
Gene name	heat shock protein family A (Hsp70) member 1A
Gene symbol	HSPA1A
Other names/aliases	HEL-S-103 HSP70 HSP70-1 HSP70-1A HSP70-2 HSP70.1 HSP70.2 HSP70I HSP72 HSPA1
Species	Homo sapiens
Experiment description of studies that identified HSPA1A in Plasma
1 Experiment ID 354 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry [Orbitrap Velos] Western blotting PubMed ID 24115447    Organism Homo sapiens Experiment description Comparative proteomics evaluation of plasma exosome isolation techniques and assessment of the stability of exosomes in normal human blood plasma Authors Mathivanan,S. Journal name Proteomics Publication year 2013 Sample Plasma Sample name Plasma - Normal Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [Orbitrap Velos] Western blotting EV-TRACK EV130073: EV-METRIC:0%, 11%, 33%, 33%, 56%
2 Experiment ID 443 Identified molecule protein Extracellular vesicle type Microparticles MISEV EVs Identification method Mass spectrometry PubMed ID 22329422    Organism Homo sapiens Experiment description Quantitative proteome profiling of normal human circulating microparticles. Authors Østergaard O, Nielsen CT, Iversen LV, Jacobsen S, Tanassi JT, Heegaard NH. Journal name J Proteome Res Publication year 2012 Sample Plasma Sample name Normal-Platelet-poor plasma Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -
3 Experiment ID 454 Identified molecule protein Extracellular vesicle type Microparticles MISEV EVs Identification method Mass spectrometry PubMed ID 22669077    Organism Homo sapiens Experiment description The proteome of erythrocyte-derived microparticles from plasma: new clues for erythrocyte aging and vesiculation. Authors Bosman GJ, Lasonder E, Groenen-Döpp YA, Willekens FL, Werre JM. Journal name J Proteomics Publication year 2012 Sample Plasma Sample name Normal-Blood Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -
4 Experiment ID 473 Identified molecule protein Extracellular vesicle type Microparticles MISEV EVs Identification method Mass spectrometry PubMed ID 23056467    Organism Homo sapiens Experiment description Cause or Effect of Arteriogenesis: Compositional Alterations of Microparticles from CAD Patients Undergoing External Counterpulsation Therapy. Authors Al Kaabi A, Traupe T, Stutz M, Buchs N, Heller M. Journal name PLoS One Publication year 2012 Sample Plasma Sample name Patients of external counterpulsation therapy-Blood Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -
5 Experiment ID 3086 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 36631249    Organism Homo sapiens Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos Authors Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO. Journal name Gut Publication year 2023 Sample Plasma Sample name Healthy donors Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
6 Experiment ID 3087 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 36631249    Organism Homo sapiens Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos Authors Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO. Journal name Gut Publication year 2023 Sample Plasma Sample name Hepatocellular carcinoma patients Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Immuno-EM EV-TRACK -
7 Experiment ID 3307 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 32139701    Organism Homo sapiens Experiment description Primary tumors release ITGBL1-rich extracellular vesicles to promote distal metastatic tumor growth through fibroblast-niche formation Authors Ji Q, Zhou L, Sui H, Yang L, Wu X, Song Q, Jia R, Li R, Sun J, Wang Z, Liu N, Feng Y, Sun X, Cai G, Feng Y, Cai J, Cao Y, Cai G, Wang Y, Li Q. Journal name Nat Commun Publication year 2020 Sample Plasma Sample name Healthy volunteers Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting ELISA EV-TRACK -
8 Experiment ID 3308 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 32139701    Organism Homo sapiens Experiment description Primary tumors release ITGBL1-rich extracellular vesicles to promote distal metastatic tumor growth through fibroblast-niche formation Authors Ji Q, Zhou L, Sui H, Yang L, Wu X, Song Q, Jia R, Li R, Sun J, Wang Z, Liu N, Feng Y, Sun X, Cai G, Feng Y, Cai J, Cao Y, Cai G, Wang Y, Li Q. Journal name Nat Commun Publication year 2020 Sample Plasma Sample name Non-metastatic colorectal cancer patients Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting ELISA EV-TRACK -
9 Experiment ID 3309 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 32139701    Organism Homo sapiens Experiment description Primary tumors release ITGBL1-rich extracellular vesicles to promote distal metastatic tumor growth through fibroblast-niche formation Authors Ji Q, Zhou L, Sui H, Yang L, Wu X, Song Q, Jia R, Li R, Sun J, Wang Z, Liu N, Feng Y, Sun X, Cai G, Feng Y, Cai J, Cao Y, Cai G, Wang Y, Li Q. Journal name Nat Commun Publication year 2020 Sample Plasma Sample name Metastatic colorectal cancer patients Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
10 Experiment ID 2000 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Mass spectrometry PubMed ID 34112803    Organism Homo sapiens Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution Authors Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. Journal name Nat Commun Publication year 2021 Sample Plasma Sample name Pooled plasma from healthy subjects and breast cancer patients Isolation/purification methods Size exclusion chromatography Centrifugal ultrafiltration Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
11 Experiment ID 2848 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 35058437    Organism Homo sapiens Experiment description Circulating ACE2-expressing extracellular vesicles block broad strains of SARS-CoV-2 Authors El-Shennawy L, Hoffmann AD, Dashzeveg NK, McAndrews KM, Mehl PJ, Cornish D, Yu Z, Tokars VL, Nicolaescu V, Tomatsidou A, Mao C, Felicelli CJ, Tsai CF, Ostiguin C, Jia Y, Li L, Furlong K, Wysocki J, Luo X, Ruivo CF, Batlle D, Hope TJ, Shen Y, Chae YK, Zhang H, LeBleu VS, Shi T, Swaminathan S, Luo Y, Missiakas D, Randall GC, Demonbreun AR, Ison MG, Kalluri R, Fang D, Liu H. Journal name Nat Commun Publication year 2022 Sample Plasma Sample name NWL-001 - Lung cancer patient Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -