Gene description for HSPA4
Gene name	heat shock protein family A (Hsp70) member 4
Gene symbol	HSPA4
Other names/aliases	APG-2 HEL-S-5a HS24/P52 HSPH2 RY hsp70 hsp70RY
Species	Homo sapiens
Experiment description of studies that identified HSPA4 in Urine
1 Experiment ID 57 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 17700640    Organism Homo sapiens Experiment description CD24 is a marker of exosomes secreted into urine and amniotic fluid. Authors Keller S, Rupp C, Stoeck A, Runz S, Fogel M, Lugert S, Hager HD, Abdel-Bakky MS, Gutwein P, Altevogt P Journal name KI Publication year 2007 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.05-1.15 g/mL Molecules identified in the study Protein Methods used in the study Western blotting FACS EV-TRACK -
2 Experiment ID 437 Identified molecule protein Extracellular vesicle type Exosomes/Membrane vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 22106071    Organism Homo sapiens Experiment description Proteomic analysis of urine exosomes by multidimensional protein identification technology (MudPIT). Authors Wang Z, Hill S, Luther JM, Hachey DL, Schey KL. Journal name Proteomics Publication year 2012 Sample Urine Sample name Normal donors-Urine Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV120168: EV-METRIC:14%
3 Experiment ID 751 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 29988836    Organism Homo sapiens Experiment description Ultrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine Authors Oeyen E, Van Mol K, Baggerman G, Willems H, Boonen K, Rolfo C, Pauwels P, Jacobs A, Schildermans K, Cho WC, Mertens I. Journal name J Extracell Vesicles Publication year 2018 Sample Urine Sample name Urine - Fraction 8 and 9 pooled Isolation/purification methods Filtration Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV170025: EV-METRIC:62%
4 Experiment ID 1239 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Western blotting PubMed ID 25329303    Organism Homo sapiens Experiment description Rapid isolation of extracellular vesicles from cell culture and biological fluids using a synthetic peptide with specific affinity for heat shock proteins. Authors Ghosh A, Davey M, Chute IC, Griffiths SG, Lewis S, Chacko S, Barnett D, Crapoulet N, Fournier S, Joy A, Caissie MC, Ferguson AD, Daigle M, Meli MV, Lewis SM, Ouellette RJ. Journal name PLoS One. Publication year 2014 Sample Urine Sample name Pre-digital rectal exam Isolation/purification methods Vn96 peptide precipitation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV140120: EV-METRIC:0%, 25%, 25%, 17%, 38%, 50%, 33%
5 Experiment ID 1241 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Western blotting PubMed ID 25329303    Organism Homo sapiens Experiment description Rapid isolation of extracellular vesicles from cell culture and biological fluids using a synthetic peptide with specific affinity for heat shock proteins. Authors Ghosh A, Davey M, Chute IC, Griffiths SG, Lewis S, Chacko S, Barnett D, Crapoulet N, Fournier S, Joy A, Caissie MC, Ferguson AD, Daigle M, Meli MV, Lewis SM, Ouellette RJ. Journal name PLoS One. Publication year 2014 Sample Urine Sample name Post-digital rectal exam Isolation/purification methods Vn96 peptide precipitation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV140120: EV-METRIC:0%, 25%, 25%, 17%, 38%, 50%, 33%