Gene description for HSPA5
Gene name	heat shock protein family A (Hsp70) member 5
Gene symbol	HSPA5
Other names/aliases	BIP GRP78 HEL-S-89n
Species	Homo sapiens
Experiment description of studies that identified HSPA5 in T cells
1 Experiment ID 307 Identified molecule protein Extracellular vesicle type Microparticles MISEV EVs Identification method Mass spectrometry [MALDI TOF/TOF] Mass spectrometry [Q-TOF] PubMed ID 16342139    Organism Homo sapiens Experiment description Proteomic analysis of malignant lymphocyte membrane microparticles using double ionization coverage optimization Authors Miguet L, Pacaud K, Felden C, Hugel B, Martinez MC, Freyssinet JM, Herbrecht R, Potier N, van Dorsselaer A, Mauvieux L Journal name Proteomics Publication year 2006 Sample T cells Sample name CEM T cell - ActD activation Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [MALDI TOF/TOF] Mass spectrometry [QTOF] EV-TRACK -
2 Experiment ID 709 Identified molecule protein Extracellular vesicle type Apoptotic bodies MISEV EVs Identification method Mass spectrometry PubMed ID 28811610    Organism Homo sapiens Experiment description Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA. Authors Néth A, Orgovan N, Só BW, Osteikoetxea X, Páczi K, Szabóylor KÉ Vukman KV, Kittel Á TuriáL, Wiener Z, TóS, Drahos L, Véy K, Horvath R, BuzáEI. Journal name Sci Rep Publication year 2017 Sample T cells Sample name Jurkat cells - Control Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV170063: EV-METRIC:75%, 75%
3 Experiment ID 711 Identified molecule protein Extracellular vesicle type Microvesicles MISEV EVs Identification method Mass spectrometry PubMed ID 28811610    Organism Homo sapiens Experiment description Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA. Authors Néth A, Orgovan N, Só BW, Osteikoetxea X, Páczi K, Szabóylor KÉ Vukman KV, Kittel Á TuriáL, Wiener Z, TóS, Drahos L, Véy K, Horvath R, BuzáEI. Journal name Sci Rep Publication year 2017 Sample T cells Sample name Jurkat cells - Activated Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV170063: EV-METRIC:75%, 75%
4 Experiment ID 712 Identified molecule protein Extracellular vesicle type Apoptotic bodies MISEV EVs Identification method Mass spectrometry PubMed ID 28811610    Organism Homo sapiens Experiment description Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA. Authors Néth A, Orgovan N, Só BW, Osteikoetxea X, Páczi K, Szabóylor KÉ Vukman KV, Kittel Á TuriáL, Wiener Z, TóS, Drahos L, Véy K, Horvath R, BuzáEI. Journal name Sci Rep Publication year 2017 Sample T cells Sample name Jurkat cells - Activated Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV170063: EV-METRIC:75%, 75%
5 Experiment ID 715 Identified molecule protein Extracellular vesicle type Apoptotic bodies MISEV EVs Identification method Mass spectrometry PubMed ID 28811610    Organism Homo sapiens Experiment description Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA. Authors Néth A, Orgovan N, Só BW, Osteikoetxea X, Páczi K, Szabóylor KÉ Vukman KV, Kittel Á TuriáL, Wiener Z, TóS, Drahos L, Véy K, Horvath R, BuzáEI. Journal name Sci Rep Publication year 2017 Sample T cells Sample name Jurkat cells - Apoptotic Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV170063: EV-METRIC:75%, 75%
6 Experiment ID 1005 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 23463506    Organism Homo sapiens Experiment description The intracellular interactome of tetraspanin-enriched microdomains reveals their function as sorting machineries toward exosomes. Authors Perez-Hernandez D, Gutiéez-Váuez C, Jorge I, Ló-MartíS, Ursa A, Sáhez-Madrid F, Váuez J, Yáz-Mó Journal name J Biol Chem. Publication year 2013 Sample T cells Sample name T lymphoblasts - exosomes pulldown with EWI-2 as a bait Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV130110: EV-METRIC:22%
7 Experiment ID 1008 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 23463506    Organism Homo sapiens Experiment description The intracellular interactome of tetraspanin-enriched microdomains reveals their function as sorting machineries toward exosomes. Authors Perez-Hernandez D, Gutiéez-Váuez C, Jorge I, Ló-MartíS, Ursa A, Sáhez-Madrid F, Váuez J, Yáz-Mó Journal name J Biol Chem. Publication year 2013 Sample T cells Sample name T lymphoblasts Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130110: EV-METRIC:22%