Gene description for HSPA8
Gene name heat shock protein family A (Hsp70) member 8
Gene symbol HSPA8
Other names/aliases HEL-33
HEL-S-72p
HSC54
HSC70
HSC71
HSP71
HSP73
HSPA10
LAP-1
LAP1
NIP71
Species Homo sapiens
 Experiment description of studies that identified HSPA8 in Ascites

1
Experiment ID56
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 12147373   
OrganismHomo sapiens
Experiment descriptionMalignant effusions and immunogenic tumour-derived exosomes.
AuthorsAndre F, Schartz NE, Movassagh M, Flament C, Pautier P, Morice P, Pomel C, Lhomme C, Escudier B, Le Chevalier T, Tursz T, Amigorena S, Raposo G, Angevin E, Zitvogel L
Journal name LANCET
Publication year2002
SampleAscites
Sample nameMalignant ascites
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density1.14-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

2
Experiment ID157
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 21630462   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of microvesicles derived from human colorectal cancer ascites
AuthorsChoi DS, Park JO, Jang SC, Yoon YJ, Jung JW, Choi DY, Kim JW, Kang JS, Park J, Hwang D, Lee KH, Park SH, Kim YK, Desiderio DM, Kim KP, Gho YS
Journal name Proteomics
Publication year2011
SampleAscites
Sample nameMalignant ascites - Colorectal cancer patient 1
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
OptiPrep density gradient
Flotation density1.09 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK EV110017: EV-METRIC:38%

3
Experiment ID158
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 21630462   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of microvesicles derived from human colorectal cancer ascites
AuthorsChoi DS, Park JO, Jang SC, Yoon YJ, Jung JW, Choi DY, Kim JW, Kang JS, Park J, Hwang D, Lee KH, Park SH, Kim YK, Desiderio DM, Kim KP, Gho YS
Journal name Proteomics
Publication year2011
SampleAscites
Sample nameMalignant ascites - Colorectal cancer patient 2
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
OptiPrep density gradient
Flotation density1.09 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK EV110017: EV-METRIC:38%

4
Experiment ID159
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 21630462   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of microvesicles derived from human colorectal cancer ascites
AuthorsChoi DS, Park JO, Jang SC, Yoon YJ, Jung JW, Choi DY, Kim JW, Kang JS, Park J, Hwang D, Lee KH, Park SH, Kim YK, Desiderio DM, Kim KP, Gho YS
Journal name Proteomics
Publication year2011
SampleAscites
Sample nameMalignant ascites - Colorectal cancer patient 3
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
OptiPrep density gradient
Flotation density1.09 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK EV110017: EV-METRIC:38%

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