Gene description for APP
Gene name	amyloid beta precursor protein
Gene symbol	APP
Other names/aliases	AAA ABETA ABPP AD1 APPI CTFgamma CVAP PN-II PN2 alpha-sAPP preA4
Species	Homo sapiens
Experiment description of studies that identified APP in Colorectal cancer cells
1 Experiment ID 303 Identified molecule mrna Extracellular vesicle type Microvesicles MISEV EVs Identification method Microarray [Illumina] PubMed ID 19930720    Organism Homo sapiens Experiment description Colorectal cancer cell-derived microvesicles are enriched in cell cycle-related mRNAs that promote proliferation of endothelial cells Authors Hong BS, Cho JH, Kim H, Choi EJ, Rho S, Kim J, Kim JH, Choi DS, Kim YK, Hwang D, Gho YS Journal name BMC Genomics Publication year 2009 Sample Colorectal cancer cells Sample name SW480 Isolation/purification methods Differential centrifugation Ultrafiltration OptiPrep density gradient Flotation density 1.09 g/mL Molecules identified in the study Protein mRNA Methods used in the study Western blotting RT-PCR Microarray [Illumina] EV-TRACK -
2 Experiment ID 480 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 23161513    Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130004: EV-METRIC:44%
3 Experiment ID 481 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 23161513    Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130004: EV-METRIC:44%
4 Experiment ID 482 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 23161513    Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130004: EV-METRIC:44%
5 Experiment ID 487 Identified molecule protein Extracellular vesicle type Exosomes/Extracellular vesicles/Microvesicles MISEV EVs Identification method Mass spectrometry PubMed ID 23230278    Organism Homo sapiens Experiment description Two distinct populations of exosomes are released from LIM1863 colon carcinoma cell-derived organoids. Authors Tauro BJ, Greening DW, Mathias RA, Mathivanan S, Ji H, Simpson RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name EpCAM affinity purified exosomes-Colorectal cancer cells (LIM1863) Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130020: EV-METRIC:38%
6 Experiment ID 488 Identified molecule protein Extracellular vesicle type Exosomes/Extracellular vesicles/Microvesicles MISEV EVs Identification method Mass spectrometry PubMed ID 23230278    Organism Homo sapiens Experiment description Two distinct populations of exosomes are released from LIM1863 colon carcinoma cell-derived organoids. Authors Tauro BJ, Greening DW, Mathias RA, Mathivanan S, Ji H, Simpson RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Shed vesicles-Colorectal cancer cells (LIM1863) Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130020: EV-METRIC:38%
7 Experiment ID 517 Identified molecule protein Extracellular vesicle type Exosomes/Membrane vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 23585443    Organism Homo sapiens Experiment description Proteome profiling of exosomes derived from human primary and metastatic colorectal cells reveal differential expression of key metastatic factors and signal transduction components. Authors Ji H, Greening DW, Barnes TW, Lim JW, Tauro BJ, Rai A, Xu R, Adda C, Mathivanan S, Zhao W, Xue Y, Xu T, Zhu HJ, Simpson RJ. Journal name Proteomics Publication year 2013 Sample Colorectal cancer cells Sample name SW480 Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130010: EV-METRIC:44%
8 Experiment ID 518 Identified molecule protein Extracellular vesicle type Exosomes/Membrane vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 23585443    Organism Homo sapiens Experiment description Proteome profiling of exosomes derived from human primary and metastatic colorectal cells reveal differential expression of key metastatic factors and signal transduction components. Authors Ji H, Greening DW, Barnes TW, Lim JW, Tauro BJ, Rai A, Xu R, Adda C, Mathivanan S, Zhao W, Xue Y, Xu T, Zhu HJ, Simpson RJ. Journal name Proteomics Publication year 2013 Sample Colorectal cancer cells Sample name SW620 Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130010: EV-METRIC:44%
9 Experiment ID 549 Identified molecule protein Extracellular vesicle type Exosomes/Extracellular vesicles/Microvesicles/Ectosomes MISEV EVs Identification method Mass spectrometry PubMed ID 24009881    Organism Homo sapiens Experiment description Quantitative proteomics of extracellular vesicles derived from human primary and metastatic colorectal cancer cells. Authors Choi DS, Choi DY, Hong BS, Jang SC, Kim DK, Lee J, Kim YK, Kim KP, Gho YS. Journal name J Extracell Vesicles Publication year 2012 Sample Colorectal cancer cells Sample name SW480 Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV120013: EV-METRIC:50%
10 Experiment ID 550 Identified molecule protein Extracellular vesicle type Exosomes/Extracellular vesicles/Microvesicles/Ectosomes MISEV EVs Identification method Mass spectrometry PubMed ID 24009881    Organism Homo sapiens Experiment description Quantitative proteomics of extracellular vesicles derived from human primary and metastatic colorectal cancer cells. Authors Choi DS, Choi DY, Hong BS, Jang SC, Kim DK, Lee J, Kim YK, Kim KP, Gho YS. Journal name J Extracell Vesicles Publication year 2012 Sample Colorectal cancer cells Sample name SW620 Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV120013: EV-METRIC:50%
11 Experiment ID 587 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Colorectal cancer cells Sample name Colo205 Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
12 Experiment ID 588 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Colorectal cancer cells Sample name HCC 2998 Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
13 Experiment ID 589 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Colorectal cancer cells Sample name HCT-116 Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
14 Experiment ID 3338 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 34887515    Organism Homo sapiens Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ. Journal name Nat Cell Biol Publication year 2021 Sample Colorectal cancer cells Sample name DiFi Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting Mass spectrometry RNA sequencing EV-TRACK -
15 Experiment ID 3339 Identified molecule protein Extracellular vesicle type Exomeres MISEV EV-like nanoparticles Identification method Western blotting PubMed ID 34887515    Organism Homo sapiens Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ. Journal name Nat Cell Biol Publication year 2021 Sample Colorectal cancer cells Sample name DiFi Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting Mass spectrometry ELISA RNA sequencing EV-TRACK -
16 Experiment ID 3340 Identified molecule protein Extracellular vesicle type Supermeres MISEV EV-like nanoparticles Identification method Western blotting PubMed ID 34887515    Organism Homo sapiens Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ. Journal name Nat Cell Biol Publication year 2021 Sample Colorectal cancer cells Sample name DiFi Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting Mass spectrometry ELISA RNA sequencing EV-TRACK -
17 Experiment ID 3351 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 34887515    Organism Homo sapiens Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ. Journal name Nat Cell Biol Publication year 2021 Sample Colorectal cancer cells Sample name HCA-7 SC Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
18 Experiment ID 3352 Identified molecule protein Extracellular vesicle type Exomeres MISEV EV-like nanoparticles Identification method Western blotting PubMed ID 34887515    Organism Homo sapiens Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ. Journal name Nat Cell Biol Publication year 2021 Sample Colorectal cancer cells Sample name HCA-7 SC Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
19 Experiment ID 3353 Identified molecule protein Extracellular vesicle type Supermeres MISEV EV-like nanoparticles Identification method Western blotting PubMed ID 34887515    Organism Homo sapiens Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ. Journal name Nat Cell Biol Publication year 2021 Sample Colorectal cancer cells Sample name HCA-7 SC Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
20 Experiment ID 3339 Identified molecule protein Extracellular vesicle type Exomeres MISEV EV-like nanoparticles Identification method Mass spectrometry PubMed ID 34887515    Organism Homo sapiens Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ. Journal name Nat Cell Biol Publication year 2021 Sample Colorectal cancer cells Sample name DiFi Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting Mass spectrometry ELISA RNA sequencing EV-TRACK -
21 Experiment ID 3340 Identified molecule protein Extracellular vesicle type Supermeres MISEV EV-like nanoparticles Identification method Mass spectrometry PubMed ID 34887515    Organism Homo sapiens Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ. Journal name Nat Cell Biol Publication year 2021 Sample Colorectal cancer cells Sample name DiFi Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting Mass spectrometry ELISA RNA sequencing EV-TRACK -