Gene description for KRT12
Gene name	keratin 12
Gene symbol	KRT12
Other names/aliases	K12 MECD1
Species	Homo sapiens
Experiment description of studies that identified KRT12 in Colorectal cancer cells
1 Experiment ID 349 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry [Orbitrap] PubMed ID 22285593    Organism Homo sapiens Experiment description Comparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes. Authors Tauro BJ, Greening DW, Mathias RA, Ji H, Mathivanan S, Scott AM, Simpson RJ Journal name Methods Publication year 2012 Sample Colorectal cancer cells Sample name LIM1863 - OptiPrep density gradient Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV120024: EV-METRIC:25%, 33%, 50%
2 Experiment ID 348 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry [Orbitrap] PubMed ID 22285593    Organism Homo sapiens Experiment description Comparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes. Authors Tauro BJ, Greening DW, Mathias RA, Ji H, Mathivanan S, Scott AM, Simpson RJ Journal name Methods Publication year 2012 Sample Colorectal cancer cells Sample name LIM1863 - EpCAM immunoaffinity Isolation/purification methods Differential centrifugation Filtration Immunoaffinity (EpCAM) Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV120024: EV-METRIC:25%, 33%, 50%
3 Experiment ID 487 Identified molecule protein Extracellular vesicle type Exosomes/Extracellular vesicles/Microvesicles MISEV EVs Identification method Mass spectrometry PubMed ID 23230278    Organism Homo sapiens Experiment description Two distinct populations of exosomes are released from LIM1863 colon carcinoma cell-derived organoids. Authors Tauro BJ, Greening DW, Mathias RA, Mathivanan S, Ji H, Simpson RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name EpCAM affinity purified exosomes-Colorectal cancer cells (LIM1863) Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130020: EV-METRIC:38%
4 Experiment ID 488 Identified molecule protein Extracellular vesicle type Exosomes/Extracellular vesicles/Microvesicles MISEV EVs Identification method Mass spectrometry PubMed ID 23230278    Organism Homo sapiens Experiment description Two distinct populations of exosomes are released from LIM1863 colon carcinoma cell-derived organoids. Authors Tauro BJ, Greening DW, Mathias RA, Mathivanan S, Ji H, Simpson RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Shed vesicles-Colorectal cancer cells (LIM1863) Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130020: EV-METRIC:38%
5 Experiment ID 517 Identified molecule protein Extracellular vesicle type Exosomes/Membrane vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 23585443    Organism Homo sapiens Experiment description Proteome profiling of exosomes derived from human primary and metastatic colorectal cells reveal differential expression of key metastatic factors and signal transduction components. Authors Ji H, Greening DW, Barnes TW, Lim JW, Tauro BJ, Rai A, Xu R, Adda C, Mathivanan S, Zhao W, Xue Y, Xu T, Zhu HJ, Simpson RJ. Journal name Proteomics Publication year 2013 Sample Colorectal cancer cells Sample name SW480 Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130010: EV-METRIC:44%
6 Experiment ID 518 Identified molecule protein Extracellular vesicle type Exosomes/Membrane vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 23585443    Organism Homo sapiens Experiment description Proteome profiling of exosomes derived from human primary and metastatic colorectal cells reveal differential expression of key metastatic factors and signal transduction components. Authors Ji H, Greening DW, Barnes TW, Lim JW, Tauro BJ, Rai A, Xu R, Adda C, Mathivanan S, Zhao W, Xue Y, Xu T, Zhu HJ, Simpson RJ. Journal name Proteomics Publication year 2013 Sample Colorectal cancer cells Sample name SW620 Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130010: EV-METRIC:44%
7 Experiment ID 696 Identified molecule protein Extracellular vesicle type Microvesicles MISEV EVs Identification method Mass spectrometry PubMed ID 28842968    Organism Homo sapiens Experiment description Insulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles. Authors Liem M, Ang CS, Mathivanan S. Journal name Proteomics Publication year 2017 Sample Colorectal cancer cells Sample name LIM1215- insulin induced Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV170021: EV-METRIC:44%, 44%
8 Experiment ID 697 Identified molecule protein Extracellular vesicle type Microvesicles MISEV EVs Identification method Mass spectrometry PubMed ID 28842968    Organism Homo sapiens Experiment description Insulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles. Authors Liem M, Ang CS, Mathivanan S. Journal name Proteomics Publication year 2017 Sample Colorectal cancer cells Sample name LIM1215- non-induced Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV170021: EV-METRIC:44%, 44%
9 Experiment ID 1049 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 28842968    Organism Homo sapiens Experiment description Insulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles. Authors Liem M, Ang CS, Mathivanan S. Journal name Proteomics. Publication year 2017 Sample Colorectal cancer cells Sample name LIM1215 - NI Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV170021: EV-METRIC:44%, 44%
10 Experiment ID 1050 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 28842968    Organism Homo sapiens Experiment description Insulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles. Authors Liem M, Ang CS, Mathivanan S. Journal name Proteomics. Publication year 2017 Sample Colorectal cancer cells Sample name lim1215 - II Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV170021: EV-METRIC:44%, 44%