Gene description for RAC1
Gene name	Rac family small GTPase 1
Gene symbol	RAC1
Other names/aliases	RAC2
Species	Canis lupus familiaris
Experiment description of studies that identified RAC1 in Kidney cells
1 Experiment ID 521 Identified molecule protein Extracellular vesicle type Exosomes/Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 23645497    Organism Canis lupus familiaris Experiment description Oncogenic H-Ras reprograms Madin-Darby canine kidney (MDCK) cell-derived exosomal proteins following epithelial-mesenchymal transition. Authors Tauro BJ, Mathias RA, Greening DW, Gopal SK, Ji H, Kapp EA, Coleman BM, Hill AF, Kusebauch U, Hallows JL, Shteynberg D, Moritz RL, Zhu HJ, Simpson RJ. Journal name Mol Cell Proteomics Publication year 2013 Sample Kidney cells Sample name Normal-Madin-Darby canine kidney cell (MDCK) Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130021: EV-METRIC:50%
2 Experiment ID 522 Identified molecule protein Extracellular vesicle type Exosomes/Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 23645497    Organism Canis lupus familiaris Experiment description Oncogenic H-Ras reprograms Madin-Darby canine kidney (MDCK) cell-derived exosomal proteins following epithelial-mesenchymal transition. Authors Tauro BJ, Mathias RA, Greening DW, Gopal SK, Ji H, Kapp EA, Coleman BM, Hill AF, Kusebauch U, Hallows JL, Shteynberg D, Moritz RL, Zhu HJ, Simpson RJ. Journal name Mol Cell Proteomics Publication year 2013 Sample Kidney cells Sample name Ras-transformed MDCK cells (21D1) Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130021: EV-METRIC:50%
3 Experiment ID 1547 Identified molecule Protein Extracellular vesicle type Microvesicles MISEV Large EVs Identification method Mass spectrometry PubMed ID 33638284    Organism Canis lupus familiaris Experiment description Transglutaminase-2, RNA-binding proteins and mitochondrial proteins selectively traffic to MDCK cell-derived microvesicles following H-Ras-induced epithelial-mesenchymal transition Authors Shafiq A, Suwakulsiri W, Rai A, Chen M, Greening DW, Zhu HJ, Xu R, Simpson RJ. Journal name Proteomics Publication year 2021 Sample Kidney cells Sample name MDCK - 10K pellet Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
4 Experiment ID 1547 Identified molecule Protein Extracellular vesicle type Microvesicles MISEV Large EVs Identification method Mass spectrometry PubMed ID 33638284    Organism Canis lupus familiaris Experiment description Transglutaminase-2, RNA-binding proteins and mitochondrial proteins selectively traffic to MDCK cell-derived microvesicles following H-Ras-induced epithelial-mesenchymal transition Authors Shafiq A, Suwakulsiri W, Rai A, Chen M, Greening DW, Zhu HJ, Xu R, Simpson RJ. Journal name Proteomics Publication year 2021 Sample Kidney cells Sample name MDCK - 10K pellet Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
5 Experiment ID 1549 Identified molecule Protein Extracellular vesicle type Microvesicles MISEV Large EVs Identification method Mass spectrometry PubMed ID 33638284    Organism Canis lupus familiaris Experiment description Transglutaminase-2, RNA-binding proteins and mitochondrial proteins selectively traffic to MDCK cell-derived microvesicles following H-Ras-induced epithelial-mesenchymal transition Authors Shafiq A, Suwakulsiri W, Rai A, Chen M, Greening DW, Zhu HJ, Xu R, Simpson RJ. Journal name Proteomics Publication year 2021 Sample Kidney cells Sample name MDCK (21D1) - 10K pellet Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
6 Experiment ID 1549 Identified molecule Protein Extracellular vesicle type Microvesicles MISEV Large EVs Identification method Mass spectrometry PubMed ID 33638284    Organism Canis lupus familiaris Experiment description Transglutaminase-2, RNA-binding proteins and mitochondrial proteins selectively traffic to MDCK cell-derived microvesicles following H-Ras-induced epithelial-mesenchymal transition Authors Shafiq A, Suwakulsiri W, Rai A, Chen M, Greening DW, Zhu HJ, Xu R, Simpson RJ. Journal name Proteomics Publication year 2021 Sample Kidney cells Sample name MDCK (21D1) - 10K pellet Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -