Gene description for P4HB
Gene name prolyl 4-hydroxylase subunit beta
Gene symbol P4HB
Other names/aliases CLCRP1
DSI
ERBA2L
GIT
P4Hbeta
PDI
PDIA1
PHDB
PO4DB
PO4HB
PROHB
Species Homo sapiens
 Experiment description of studies that identified P4HB in Gastric cancer cells

1
Experiment ID2900
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33732415   
OrganismHomo sapiens
Experiment descriptionExtracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway
AuthorsGao X, Wang Y, Lu F, Chen X, Yang D, Cao Y, Zhang W, Chen J, Zheng L, Wang G, Fu M, Ma L, Song Y, Zhan Q.
Journal name J Extracell Vesicles
Publication year2021
SampleGastric cancer cells
Sample nameAGS
Isolation/purification methodsFiltration
Centrifugal ultrafiltration
ExoQuick Kit
Differential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

2
Experiment ID1493
Identified moleculeProtein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 33991177   
OrganismHomo sapiens
Experiment descriptionCancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
AuthorsCarli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year2021
SampleGastric cancer cells
Sample nameMKN1 - 100K pellet
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

3
Experiment ID1494
Identified moleculeProtein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 33991177   
OrganismHomo sapiens
Experiment descriptionCancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
AuthorsCarli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year2021
SampleGastric cancer cells
Sample nameMKN1 - 100K pellet
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

4
Experiment ID1495
Identified moleculeProtein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 33991177   
OrganismHomo sapiens
Experiment descriptionCancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
AuthorsCarli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year2021
SampleGastric cancer cells
Sample nameMKN1 - 100K pellet
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

<