Gene description for Smpd3
Gene name	sphingomyelin phosphodiesterase 3, neutral
Gene symbol	Smpd3
Other names/aliases	4631433G07Rik fro nSMase2
Species	Mus musculus
Experiment description of studies that identified Smpd3 in Adipocytes
1 Experiment ID 2366 Identified molecule Protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 36759608    Organism Mus musculus Experiment description Adipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo Authors Kulaj K, Harger A, Bauer M, Caliskan ÖS, Gupta TK, Chiang DM, Milbank E, Reber J, Karlas A, Kotzbeck P, Sailer DN, Volta F, Lutter D, Prakash S, Merl-Pham J, Ntziachristos V, Hauner H, Pfaffl MW, Tschöp MH, Müller TD, Hauck SM, Engel BD, Gerdes JM, Pfluger PT, Krahmer N, Stemmer K. Journal name Nat Commun Publication year 2023 Sample Adipocytes Sample name C57BL/6 J eWAT-derived adipocytes - Lean Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Mass spectrometry RT-PCR EV-TRACK -
2 Experiment ID 2367 Identified molecule Protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 36759608    Organism Mus musculus Experiment description Adipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo Authors Kulaj K, Harger A, Bauer M, Caliskan ÖS, Gupta TK, Chiang DM, Milbank E, Reber J, Karlas A, Kotzbeck P, Sailer DN, Volta F, Lutter D, Prakash S, Merl-Pham J, Ntziachristos V, Hauner H, Pfaffl MW, Tschöp MH, Müller TD, Hauck SM, Engel BD, Gerdes JM, Pfluger PT, Krahmer N, Stemmer K. Journal name Nat Commun Publication year 2023 Sample Adipocytes Sample name C57BL/6 J eWAT-derived adipocytes - Lean Isolation/purification methods Centrifugal ultrafiltration Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK -
3 Experiment ID 2368 Identified molecule Protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 36759608    Organism Mus musculus Experiment description Adipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo Authors Kulaj K, Harger A, Bauer M, Caliskan ÖS, Gupta TK, Chiang DM, Milbank E, Reber J, Karlas A, Kotzbeck P, Sailer DN, Volta F, Lutter D, Prakash S, Merl-Pham J, Ntziachristos V, Hauner H, Pfaffl MW, Tschöp MH, Müller TD, Hauck SM, Engel BD, Gerdes JM, Pfluger PT, Krahmer N, Stemmer K. Journal name Nat Commun Publication year 2023 Sample Adipocytes Sample name C57BL/6 J eWAT-derived adipocytes - Diet-induced obesity Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Mass spectrometry RT-PCR EV-TRACK -
4 Experiment ID 2369 Identified molecule Protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 36759608    Organism Mus musculus Experiment description Adipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo Authors Kulaj K, Harger A, Bauer M, Caliskan ÖS, Gupta TK, Chiang DM, Milbank E, Reber J, Karlas A, Kotzbeck P, Sailer DN, Volta F, Lutter D, Prakash S, Merl-Pham J, Ntziachristos V, Hauner H, Pfaffl MW, Tschöp MH, Müller TD, Hauck SM, Engel BD, Gerdes JM, Pfluger PT, Krahmer N, Stemmer K. Journal name Nat Commun Publication year 2023 Sample Adipocytes Sample name C57BL/6 J eWAT-derived adipocytes - Diet-induced obesity Isolation/purification methods Centrifugal ultrafiltration Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK -