Gene description for RPL18
Gene name	ribosomal protein L18
Gene symbol	RPL18
Other names/aliases	DBA18 L18 eL18
Species	Homo sapiens
Experiment description of studies that identified RPL18 in Colorectal cancer cells
1 Experiment ID 347 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry [Orbitrap] PubMed ID 22285593    Organism Homo sapiens Experiment description Comparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes. Authors Tauro BJ, Greening DW, Mathias RA, Ji H, Mathivanan S, Scott AM, Simpson RJ Journal name Methods Publication year 2012 Sample Colorectal cancer cells Sample name LIM1863 - Ultracentrifugation Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV120024: EV-METRIC:25%, 33%, 50%
2 Experiment ID 348 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry [Orbitrap] PubMed ID 22285593    Organism Homo sapiens Experiment description Comparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes. Authors Tauro BJ, Greening DW, Mathias RA, Ji H, Mathivanan S, Scott AM, Simpson RJ Journal name Methods Publication year 2012 Sample Colorectal cancer cells Sample name LIM1863 - EpCAM immunoaffinity Isolation/purification methods Differential centrifugation Filtration Immunoaffinity (EpCAM) Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV120024: EV-METRIC:25%, 33%, 50%
3 Experiment ID 486 Identified molecule protein Extracellular vesicle type Exosomes/Extracellular vesicles/Microvesicles MISEV EVs Identification method Mass spectrometry PubMed ID 23230278    Organism Homo sapiens Experiment description Two distinct populations of exosomes are released from LIM1863 colon carcinoma cell-derived organoids. Authors Tauro BJ, Greening DW, Mathias RA, Mathivanan S, Ji H, Simpson RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name A33 affinity purified exosomes-Colorectal cancer cells (LIM1863) Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130020: EV-METRIC:38%
4 Experiment ID 487 Identified molecule protein Extracellular vesicle type Exosomes/Extracellular vesicles/Microvesicles MISEV EVs Identification method Mass spectrometry PubMed ID 23230278    Organism Homo sapiens Experiment description Two distinct populations of exosomes are released from LIM1863 colon carcinoma cell-derived organoids. Authors Tauro BJ, Greening DW, Mathias RA, Mathivanan S, Ji H, Simpson RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name EpCAM affinity purified exosomes-Colorectal cancer cells (LIM1863) Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130020: EV-METRIC:38%
5 Experiment ID 488 Identified molecule protein Extracellular vesicle type Exosomes/Extracellular vesicles/Microvesicles MISEV EVs Identification method Mass spectrometry PubMed ID 23230278    Organism Homo sapiens Experiment description Two distinct populations of exosomes are released from LIM1863 colon carcinoma cell-derived organoids. Authors Tauro BJ, Greening DW, Mathias RA, Mathivanan S, Ji H, Simpson RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Shed vesicles-Colorectal cancer cells (LIM1863) Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130020: EV-METRIC:38%
6 Experiment ID 517 Identified molecule protein Extracellular vesicle type Exosomes/Membrane vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 23585443    Organism Homo sapiens Experiment description Proteome profiling of exosomes derived from human primary and metastatic colorectal cells reveal differential expression of key metastatic factors and signal transduction components. Authors Ji H, Greening DW, Barnes TW, Lim JW, Tauro BJ, Rai A, Xu R, Adda C, Mathivanan S, Zhao W, Xue Y, Xu T, Zhu HJ, Simpson RJ. Journal name Proteomics Publication year 2013 Sample Colorectal cancer cells Sample name SW480 Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130010: EV-METRIC:44%
7 Experiment ID 518 Identified molecule protein Extracellular vesicle type Exosomes/Membrane vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 23585443    Organism Homo sapiens Experiment description Proteome profiling of exosomes derived from human primary and metastatic colorectal cells reveal differential expression of key metastatic factors and signal transduction components. Authors Ji H, Greening DW, Barnes TW, Lim JW, Tauro BJ, Rai A, Xu R, Adda C, Mathivanan S, Zhao W, Xue Y, Xu T, Zhu HJ, Simpson RJ. Journal name Proteomics Publication year 2013 Sample Colorectal cancer cells Sample name SW620 Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130010: EV-METRIC:44%
8 Experiment ID 588 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Colorectal cancer cells Sample name HCC 2998 Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
9 Experiment ID 589 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Colorectal cancer cells Sample name HCT-116 Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
10 Experiment ID 590 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Colorectal cancer cells Sample name HCT-15 Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
11 Experiment ID 696 Identified molecule protein Extracellular vesicle type Microvesicles MISEV EVs Identification method Mass spectrometry PubMed ID 28842968    Organism Homo sapiens Experiment description Insulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles. Authors Liem M, Ang CS, Mathivanan S. Journal name Proteomics Publication year 2017 Sample Colorectal cancer cells Sample name LIM1215- insulin induced Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV170021: EV-METRIC:44%, 44%
12 Experiment ID 697 Identified molecule protein Extracellular vesicle type Microvesicles MISEV EVs Identification method Mass spectrometry PubMed ID 28842968    Organism Homo sapiens Experiment description Insulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles. Authors Liem M, Ang CS, Mathivanan S. Journal name Proteomics Publication year 2017 Sample Colorectal cancer cells Sample name LIM1215- non-induced Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV170021: EV-METRIC:44%, 44%
13 Experiment ID 1049 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 28842968    Organism Homo sapiens Experiment description Insulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles. Authors Liem M, Ang CS, Mathivanan S. Journal name Proteomics. Publication year 2017 Sample Colorectal cancer cells Sample name LIM1215 - NI Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV170021: EV-METRIC:44%, 44%
14 Experiment ID 1050 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 28842968    Organism Homo sapiens Experiment description Insulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles. Authors Liem M, Ang CS, Mathivanan S. Journal name Proteomics. Publication year 2017 Sample Colorectal cancer cells Sample name lim1215 - II Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV170021: EV-METRIC:44%, 44%
15 Experiment ID 3338 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 34887515    Organism Homo sapiens Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ. Journal name Nat Cell Biol Publication year 2021 Sample Colorectal cancer cells Sample name DiFi Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting Mass spectrometry RNA sequencing EV-TRACK -
16 Experiment ID 3339 Identified molecule protein Extracellular vesicle type Exomeres MISEV EV-like nanoparticles Identification method Mass spectrometry PubMed ID 34887515    Organism Homo sapiens Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ. Journal name Nat Cell Biol Publication year 2021 Sample Colorectal cancer cells Sample name DiFi Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting Mass spectrometry ELISA RNA sequencing EV-TRACK -