Gene description for Pfn1
Gene name	profilin 1
Gene symbol	Pfn1
Other names/aliases	-
Species	Rattus norvegicus
Experiment description of studies that identified Pfn1 in Insulinoma cells
1 Experiment ID 1220 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 27872147    Organism Rattus norvegicus Experiment description Primary Human and Rat .-Cells Release the Intracellular Autoantigens GAD65, IA-2, and Proinsulin in Exosomes Together With Cytokine-Induced Enhancers of Immunity. Authors Cianciaruso C, Phelps EA, Pasquier M, Hamelin R, Demurtas D, Alibashe Ahmed M, Piemonti L, Hirosue S, Swartz MA, De Palma M, Hubbell JA, Baekkeskov S. Journal name Diabetes. Publication year 2017 Sample Insulinoma cells Sample name INS-1E cells Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV160008: EV-METRIC:55%, 44%, 33%
2 Experiment ID 1221 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 27872147    Organism Rattus norvegicus Experiment description Primary Human and Rat .-Cells Release the Intracellular Autoantigens GAD65, IA-2, and Proinsulin in Exosomes Together With Cytokine-Induced Enhancers of Immunity. Authors Cianciaruso C, Phelps EA, Pasquier M, Hamelin R, Demurtas D, Alibashe Ahmed M, Piemonti L, Hirosue S, Swartz MA, De Palma M, Hubbell JA, Baekkeskov S. Journal name Diabetes. Publication year 2017 Sample Insulinoma cells Sample name Rat interleukin (IL)-1. (10 units/mL) Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV160008: EV-METRIC:55%, 44%, 33%
3 Experiment ID 1280 Identified molecule protein Extracellular vesicle type Microparticles MISEV EVs Identification method Mass spectrometry PubMed ID 22345510    Organism Rattus norvegicus Experiment description Characterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies. Authors Palmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR. Journal name Mol Cell Proteomics. Publication year 2012 Sample Insulinoma cells Sample name NHI 6F Tu28, cytokine-stimulated Replicate 2 Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV120151: EV-METRIC:14%, 0%