Gene description for Flot1
Gene name flotillin 1
Gene symbol Flot1
Other names/aliases Rareg
Species Rattus norvegicus
 Experiment description of studies that identified Flot1 in Islets

1
Experiment ID1215
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 27872147   
OrganismRattus norvegicus
Experiment descriptionPrimary Human and Rat .-Cells Release the Intracellular Autoantigens GAD65, IA-2, and Proinsulin in Exosomes Together With Cytokine-Induced Enhancers of Immunity.
AuthorsCianciaruso C, Phelps EA, Pasquier M, Hamelin R, Demurtas D, Alibashe Ahmed M, Piemonti L, Hirosue S, Swartz MA, De Palma M, Hubbell JA, Baekkeskov S.
Journal name Diabetes.
Publication year2017
SampleIslets
Sample nameRat interleukin (IL)-1. (10 units/mL)- 4h
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK EV160008: EV-METRIC:55%, 44%, 33%

2
Experiment ID1216
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 27872147   
OrganismRattus norvegicus
Experiment descriptionPrimary Human and Rat .-Cells Release the Intracellular Autoantigens GAD65, IA-2, and Proinsulin in Exosomes Together With Cytokine-Induced Enhancers of Immunity.
AuthorsCianciaruso C, Phelps EA, Pasquier M, Hamelin R, Demurtas D, Alibashe Ahmed M, Piemonti L, Hirosue S, Swartz MA, De Palma M, Hubbell JA, Baekkeskov S.
Journal name Diabetes.
Publication year2017
SampleIslets
Sample nameRat interleukin (IL)-1. (10 units/mL)- 8h
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK EV160008: EV-METRIC:55%, 44%, 33%

3
Experiment ID1217
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 27872147   
OrganismRattus norvegicus
Experiment descriptionPrimary Human and Rat .-Cells Release the Intracellular Autoantigens GAD65, IA-2, and Proinsulin in Exosomes Together With Cytokine-Induced Enhancers of Immunity.
AuthorsCianciaruso C, Phelps EA, Pasquier M, Hamelin R, Demurtas D, Alibashe Ahmed M, Piemonti L, Hirosue S, Swartz MA, De Palma M, Hubbell JA, Baekkeskov S.
Journal name Diabetes.
Publication year2017
SampleIslets
Sample nameRat interleukin (IL)-1. (10 units/mL)- 24h
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK EV160008: EV-METRIC:55%, 44%, 33%

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