Gene description for Idh3a
Gene name	isocitrate dehydrogenase 3 (NAD+) alpha
Gene symbol	Idh3a
Other names/aliases	1110003P10Rik 1500012E04Rik
Species	Mus musculus
Experiment description of studies that identified Idh3a in Adipocytes
1 Experiment ID 304 Identified molecule protein Extracellular vesicle type Microvesicles MISEV EVs Identification method Mass spectrometry [LCQ-DECA XP] PubMed ID 17478559    Organism Mus musculus Experiment description Identification and characterization of microvesicles secreted by 3T3-L1 adipocytes: redox- and hormone-dependent induction of milk fat globule-epidermal growth 8-associated microvesicles Authors Aoki N, Jin-no S, Nakagawa Y, Asai N, Arakawa E, Tamura N, Tamura T, Matsuda T Journal name Endocrinology Publication year 2007 Sample Adipocytes Sample name 3T3-L1 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.13–-1.20 g/mL Molecules identified in the study Protein Methods used in the study Immunoelectron microscopy Western blotting Mass spectrometry [LCQ-DECA XP] EV-TRACK -
2 Experiment ID 2366 Identified molecule Protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 36759608    Organism Mus musculus Experiment description Adipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo Authors Kulaj K, Harger A, Bauer M, Caliskan ÖS, Gupta TK, Chiang DM, Milbank E, Reber J, Karlas A, Kotzbeck P, Sailer DN, Volta F, Lutter D, Prakash S, Merl-Pham J, Ntziachristos V, Hauner H, Pfaffl MW, Tschöp MH, Müller TD, Hauck SM, Engel BD, Gerdes JM, Pfluger PT, Krahmer N, Stemmer K. Journal name Nat Commun Publication year 2023 Sample Adipocytes Sample name C57BL/6 J eWAT-derived adipocytes - Lean Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Mass spectrometry RT-PCR EV-TRACK -
3 Experiment ID 2367 Identified molecule Protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 36759608    Organism Mus musculus Experiment description Adipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo Authors Kulaj K, Harger A, Bauer M, Caliskan ÖS, Gupta TK, Chiang DM, Milbank E, Reber J, Karlas A, Kotzbeck P, Sailer DN, Volta F, Lutter D, Prakash S, Merl-Pham J, Ntziachristos V, Hauner H, Pfaffl MW, Tschöp MH, Müller TD, Hauck SM, Engel BD, Gerdes JM, Pfluger PT, Krahmer N, Stemmer K. Journal name Nat Commun Publication year 2023 Sample Adipocytes Sample name C57BL/6 J eWAT-derived adipocytes - Lean Isolation/purification methods Centrifugal ultrafiltration Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK -
4 Experiment ID 2368 Identified molecule Protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 36759608    Organism Mus musculus Experiment description Adipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo Authors Kulaj K, Harger A, Bauer M, Caliskan ÖS, Gupta TK, Chiang DM, Milbank E, Reber J, Karlas A, Kotzbeck P, Sailer DN, Volta F, Lutter D, Prakash S, Merl-Pham J, Ntziachristos V, Hauner H, Pfaffl MW, Tschöp MH, Müller TD, Hauck SM, Engel BD, Gerdes JM, Pfluger PT, Krahmer N, Stemmer K. Journal name Nat Commun Publication year 2023 Sample Adipocytes Sample name C57BL/6 J eWAT-derived adipocytes - Diet-induced obesity Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Mass spectrometry RT-PCR EV-TRACK -
5 Experiment ID 2369 Identified molecule Protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 36759608    Organism Mus musculus Experiment description Adipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo Authors Kulaj K, Harger A, Bauer M, Caliskan ÖS, Gupta TK, Chiang DM, Milbank E, Reber J, Karlas A, Kotzbeck P, Sailer DN, Volta F, Lutter D, Prakash S, Merl-Pham J, Ntziachristos V, Hauner H, Pfaffl MW, Tschöp MH, Müller TD, Hauck SM, Engel BD, Gerdes JM, Pfluger PT, Krahmer N, Stemmer K. Journal name Nat Commun Publication year 2023 Sample Adipocytes Sample name C57BL/6 J eWAT-derived adipocytes - Diet-induced obesity Isolation/purification methods Centrifugal ultrafiltration Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK -