Gene description for ACTG1
Gene name	actin gamma 1
Gene symbol	ACTG1
Other names/aliases	ACT ACTG DFNA20 DFNA26 HEL-176
Species	Homo sapiens
Experiment description of studies that identified ACTG1 in Melanoma cells
1 Experiment ID 12 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 15478216    Organism Homo sapiens Experiment description Proteomic analysis of melanoma-derived exosomes by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. Authors Mears R, Craven RA, Hanrahan S, Totty N, Upton C, Young SL, Patel P, Selby PJ, Banks RE Journal name PROTEOMICS Publication year 2004 Sample Melanoma cells Sample name MeWo SK-MEL-28 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.20 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [4700 Proteomics Analyzer] Western blotting EV-TRACK -
2 Experiment ID 496 Identified molecule mRNA Extracellular vesicle type Microvesicles MISEV EVs Identification method Microarray PubMed ID 23344721    Organism Homo sapiens Experiment description miR-1289 and Zipcode-like Sequence Enrich mRNAs in Microvesicles. Authors Bolukbasi MF, Mizrak A, Ozdener GB, Madlener S, Ströbel T, Erkan EP, Fan JB, Breakefield XO, Saydam O. Journal name Mol Ther Nucleic Acids Publication year 2012 Sample Melanoma cells Sample name Primary malignant melanoma cells (0105) Isolation/purification methods - Flotation density - Molecules identified in the study mRNA Methods used in the study Microarray EV-TRACK -
3 Experiment ID 497 Identified molecule mRNA Extracellular vesicle type Microvesicles MISEV EVs Identification method Microarray PubMed ID 23344721    Organism Homo sapiens Experiment description miR-1289 and Zipcode-like Sequence Enrich mRNAs in Microvesicles. Authors Bolukbasi MF, Mizrak A, Ozdener GB, Madlener S, Ströbel T, Erkan EP, Fan JB, Breakefield XO, Saydam O. Journal name Mol Ther Nucleic Acids Publication year 2012 Sample Melanoma cells Sample name Primary malignant melanoma cells (0664) Isolation/purification methods - Flotation density - Molecules identified in the study mRNA Methods used in the study Microarray EV-TRACK -
4 Experiment ID 986 Identified molecule protein Extracellular vesicle type Exosomes/Microvesicles MISEV EVs Identification method Mass spectrometry PubMed ID 25261472    Organism Homo sapiens Experiment description Complex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment. Authors Liang Y, Eng WS, Colquhoun DR, Dinglasan RR, Graham DR, Mahal LK. Journal name J Biol Chem. Publication year 2014 Sample Melanoma cells Sample name SK-MEL-5 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV140188: EV-METRIC:22%