Gene description for TPI1
Gene name	triosephosphate isomerase 1
Gene symbol	TPI1
Other names/aliases	HEL-S-49 TIM TPI TPID
Species	Homo sapiens
Experiment description of studies that identified TPI1 in Melanoma cells
1 Experiment ID 12 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 15478216    Organism Homo sapiens Experiment description Proteomic analysis of melanoma-derived exosomes by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. Authors Mears R, Craven RA, Hanrahan S, Totty N, Upton C, Young SL, Patel P, Selby PJ, Banks RE Journal name PROTEOMICS Publication year 2004 Sample Melanoma cells Sample name MeWo SK-MEL-28 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.20 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [4700 Proteomics Analyzer] Western blotting EV-TRACK -
2 Experiment ID 453 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 22635005    Organism Homo sapiens Experiment description Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET. Authors Peinado H, Alecković M, Lavotshkin S, Matei I, Costa-Silva B, Moreno-Bueno G, Hergueta-Redondo M, Williams C, García-Santos G, Ghajar C, Nitadori-Hoshino A, Hoffman C, Badal K, Garcia BA, Callahan MK, Yuan J, Martins VR, Skog J, Kaplan RN, Brady MS, Wolchok JD, Chapman PB, Kang Y, Bromberg J, Lyden D. Journal name Nat Med Publication year 2012 Sample Melanoma cells Sample name B16-F10, SK-MEL-202, SK-MEL035, SK-MEL-265 Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV120019: EV-METRIC:44%, 44%, 44%
3 Experiment ID 986 Identified molecule protein Extracellular vesicle type Exosomes/Microvesicles MISEV EVs Identification method Mass spectrometry PubMed ID 25261472    Organism Homo sapiens Experiment description Complex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment. Authors Liang Y, Eng WS, Colquhoun DR, Dinglasan RR, Graham DR, Mahal LK. Journal name J Biol Chem. Publication year 2014 Sample Melanoma cells Sample name SK-MEL-5 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV140188: EV-METRIC:22%