Gene description for CD63
Gene name	CD63 molecule
Gene symbol	CD63
Other names/aliases	AD1 HOP-26 ME491 MLA1 OMA81H Pltgp40 TSPAN30
Species	Homo sapiens
Experiment description of studies that identified CD63 in Glioblastoma cells
1 Experiment ID 176 Identified molecule protein Extracellular vesicle type Microvesicles MISEV EVs Identification method Immunoelectron Microscopy Western blotting PubMed ID 21788507    Organism Homo sapiens Experiment description Hypoxia triggers a proangiogenic pathway involving cancer cell microvesicles and PAR-2-mediated heparin-binding EGF signaling in endothelial cells Authors Svensson KJ, Kucharzewska P, Christianson HC, SköS, Löedt T, Johansson MC, Mölin M, Bengzon J, Ruf W, Belting M Journal name Proc Natl Acad Sci U S A Publication year 2011 Sample Glioblastoma cells Sample name U87-MG Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Immunoelectron microscopy Western blotting EV-TRACK EV110023: EV-METRIC:33%
2 Experiment ID 203 Identified molecule mrna Extracellular vesicle type Microvesicles MISEV EVs Identification method Microarray PubMed ID 19011622    Organism Homo sapiens Experiment description Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers Authors Skog J, Wüer T, van Rijn S, Meijer DH, Gainche L, Sena-Esteves M, Curry WT Jr, Carter BS, Krichevsky AM, Breakefield XO Journal name Nat Cell Biol Publication year 2008 Sample Glioblastoma cells Sample name Glioblastoma cells Isolation/purification methods Differential centrifugation Ultracentrifugation Filtration Flotation density - Molecules identified in the study Protein miRNA mRNA Methods used in the study RT-PCR Antibody array Microarray EV-TRACK -
3 Experiment ID 723 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 25802036    Organism Homo sapiens Experiment description Glioblastoma-derived extracellular vesicles modify the phenotype of monocytic cells. Authors de Vrij J, Maas SL, Kwappenberg KM, Schnoor R, Kleijn A, Dekker L, Luider TM, de Witte LD, Litjens M, van Strien ME, Hol EM, Kroonen J, Robe PA, Lamfers ML, Schilham MW, Broekman ML. Journal name Int J Cancer Publication year 2015 Sample Glioblastoma cells Sample name GS184 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV150064: EV-METRIC:0%, 0%, 29%
4 Experiment ID 1242 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry Western blotting PubMed ID 25261722    Organism Homo sapiens Experiment description Inflammatory cytokines, interleukin-1 beta and tumor necrosis factor-alpha, upregulated in glioblastoma multiforme, raise the levels of CRYAB in exosomes secreted by U373 glioma cells. Authors Kore RA, Abraham EC. Journal name Biochem Biophys Res Commun. Publication year 2014 Sample Glioblastoma cells Sample name U373 cells treated with 10ng/ml of IL-1. Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV140183: EV-METRIC:22%
5 Experiment ID 1243 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry Western blotting PubMed ID 25261722    Organism Homo sapiens Experiment description Inflammatory cytokines, interleukin-1 beta and tumor necrosis factor-alpha, upregulated in glioblastoma multiforme, raise the levels of CRYAB in exosomes secreted by U373 glioma cells. Authors Kore RA, Abraham EC. Journal name Biochem Biophys Res Commun. Publication year 2014 Sample Glioblastoma cells Sample name U373 cells treated with 10ng/ml of TNF-. Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV140183: EV-METRIC:22%
6 Experiment ID 1067 Identified molecule protein Extracellular vesicle type Microvesicles MISEV EVs Identification method Western blotting PubMed ID 29937354    Organism Homo sapiens Experiment description Apoptotic Cell-Derived Extracellular Vesicles Promote Malignancy of Glioblastoma Via Intercellular Transfer of Splicing Factors. Authors Pavlyukov MS, Yu H, Bastola S, Minata M, Shender VO, Lee Y, Zhang S, Wang J, Komarova S, Wang J, Yamaguchi S, Alsheikh HA, Shi J, Chen D, Mohyeldin A, Kim SH, Shin YJ, Anufrieva K, Evtushenko EG, Antipova NV, Arapidi GP, Govorun V, Pestov NB, Shakhparonov MI, Lee LJ, Nam DH, Nakano I. Journal name Cancer Cell Publication year 2018 Sample Glioblastoma cells Sample name Untreated GBM157 - MVs Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK -
7 Experiment ID 1068 Identified molecule protein Extracellular vesicle type Microvesicles MISEV EVs Identification method Western blotting PubMed ID 29937354    Organism Homo sapiens Experiment description Apoptotic Cell-Derived Extracellular Vesicles Promote Malignancy of Glioblastoma Via Intercellular Transfer of Splicing Factors. Authors Pavlyukov MS, Yu H, Bastola S, Minata M, Shender VO, Lee Y, Zhang S, Wang J, Komarova S, Wang J, Yamaguchi S, Alsheikh HA, Shi J, Chen D, Mohyeldin A, Kim SH, Shin YJ, Anufrieva K, Evtushenko EG, Antipova NV, Arapidi GP, Govorun V, Pestov NB, Shakhparonov MI, Lee LJ, Nam DH, Nakano I. Journal name Cancer Cell Publication year 2018 Sample Glioblastoma cells Sample name Lethally irradiated GBM157 -MVs (apoMVs) Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK -
8 Experiment ID 1069 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 29937354    Organism Homo sapiens Experiment description Apoptotic Cell-Derived Extracellular Vesicles Promote Malignancy of Glioblastoma Via Intercellular Transfer of Splicing Factors. Authors Pavlyukov MS, Yu H, Bastola S, Minata M, Shender VO, Lee Y, Zhang S, Wang J, Komarova S, Wang J, Yamaguchi S, Alsheikh HA, Shi J, Chen D, Mohyeldin A, Kim SH, Shin YJ, Anufrieva K, Evtushenko EG, Antipova NV, Arapidi GP, Govorun V, Pestov NB, Shakhparonov MI, Lee LJ, Nam DH, Nakano I. Journal name Cancer Cell Publication year 2018 Sample Glioblastoma cells Sample name Untreated GBM157 - Exo - Biorep1 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK -
9 Experiment ID 1070 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 29937354    Organism Homo sapiens Experiment description Apoptotic Cell-Derived Extracellular Vesicles Promote Malignancy of Glioblastoma Via Intercellular Transfer of Splicing Factors. Authors Pavlyukov MS, Yu H, Bastola S, Minata M, Shender VO, Lee Y, Zhang S, Wang J, Komarova S, Wang J, Yamaguchi S, Alsheikh HA, Shi J, Chen D, Mohyeldin A, Kim SH, Shin YJ, Anufrieva K, Evtushenko EG, Antipova NV, Arapidi GP, Govorun V, Pestov NB, Shakhparonov MI, Lee LJ, Nam DH, Nakano I. Journal name Cancer Cell Publication year 2018 Sample Glioblastoma cells Sample name Untreated GBM157 - Exo - Biorep2 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -
10 Experiment ID 1265 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Western blotting PubMed ID 24310399    Organism Homo sapiens Experiment description Extracellular vesicles modulate the glioblastoma microenvironment via a tumor suppression signaling network directed by miR-1. Authors Bronisz A, Wang Y, Nowicki MO, Peruzzi P, Ansari K, Ogawa D, Balaj L, De Rienzo G, Mineo M, Nakano I, Ostrowski MC, Hochberg F, Weissleder R, Lawler SE, Chiocca EA, Godlewski J. Journal name Cancer Res. Publication year 2014 Sample Glioblastoma cells Sample name U87 cells - control Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV140062: EV-METRIC:33%
11 Experiment ID 1266 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Western blotting PubMed ID 24310399    Organism Homo sapiens Experiment description Extracellular vesicles modulate the glioblastoma microenvironment via a tumor suppression signaling network directed by miR-1. Authors Bronisz A, Wang Y, Nowicki MO, Peruzzi P, Ansari K, Ogawa D, Balaj L, De Rienzo G, Mineo M, Nakano I, Ostrowski MC, Hochberg F, Weissleder R, Lawler SE, Chiocca EA, Godlewski J. Journal name Cancer Res. Publication year 2014 Sample Glioblastoma cells Sample name U87 cells + miRNA-1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV140062: EV-METRIC:33%
12 Experiment ID 2029 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 35986010    Organism Homo sapiens Experiment description Exosomal miR-155-5p derived from glioma stem-like cells promotes mesenchymal transition via targeting ACOT12 Authors Bao Z, Zhang N, Niu W, Mu M, Zhang X, Hu S, Niu C. Journal name Cell Death Dis Publication year 2022 Sample Glioblastoma cells Sample name U87 Isolation/purification methods Centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting miRNA sequencing EV-TRACK -
13 Experiment ID 1653 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Flow cytometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Glioblastoma cells Sample name U87 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Flow cytometry EV-TRACK -
14 Experiment ID 1655 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Flow cytometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Glioblastoma cells Sample name U87 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Flow cytometry EV-TRACK -