Gene description for Cd9
Gene name CD9 antigen
Gene symbol Cd9
Other names/aliases Tspan29
Species Mus musculus
 Database cross references - Cd9
Vesiclepedia VP_12527
ExoCarta ExoCarta_12527
Entrez Gene 12527
 Cd9 identified in extracellular vesicles derived from the following tissue/cell type
Adipocytes [Microvesicles]      More >>> 17478559   
Amniotic fluid [Exosomes]      More >>> 17700640   
Brain cancer cells [Exosomes]      More >>> 19109410   
Breast cancer cells [Exosomes]      More >>> 29115712   
Breast cancer cells [Exosomes]      More >>> 29115712   
Breast cancer cells [Exosomes]      More >>> 29115712   
Breast cancer cells [Exosomes]      More >>> 29115712   
Dendritic cells [Exosomes]      More >>> 10545503   
Dendritic cells [Exosomes]      More >>> 11390481   
Embryonic fibroblasts [Exosomes]      More >>> 18494037   
Keratinocytes [Exosomes]      More >>> 24079734   
Keratinocytes [Exosomes]      More >>> 24079734   
Macrophages [Exosomes]      More >>> 23658846   
Macrophages [Exosomes]      More >>> 23658846   
Macrophages [Exosomes]      More >>> 23658846   
Melanoma cells [Extracellular vesicles]      More >>> 29907695   
Melanoma cells [Extracellular vesicles]      More >>> 29907695   
Melanoma cells [Extracellular vesicles]      More >>> 29907695   
Microglia [Exosomes]      More >>> 16081791   
Mouse embryonic fibroblasts [Extracellular vesicles]      More >>> 30035390   
Mouse embryonic fibroblasts [Extracellular vesicles]      More >>> 30035390   
Mouse embryonic fibroblasts [Extracellular vesicles]      More >>> 30035390   
Mouse embryonic fibroblasts [Extracellular vesicles]      More >>> 30035390   
Murine myoblast [Extracellular vesicles]      More >>> 24392111   
Murine myotubues [Extracellular vesicles]      More >>> 24392111   
Myeloid-derived suppressor cells [Exosomes]      More >>> 29951053   
Osteoblasts [Matrix vesicles]      More >>> 17096383   
Urine [Exosomes]      More >>> 17700640   
 Experiment description of studies that identified Cd9 in extracellular vesicles
1
Experiment ID304
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry [LCQ-DECA XP]
PubMed ID 17478559   
OrganismMus musculus
Experiment descriptionIdentification and characterization of microvesicles secreted by 3T3-L1 adipocytes: redox- and hormone-dependent induction of milk fat globule-epidermal growth 8-associated microvesicles
AuthorsAoki N, Jin-no S, Nakagawa Y, Asai N, Arakawa E, Tamura N, Tamura T, Matsuda T
Journal name Endocrinology
Publication year2007
SampleAdipocytes
Sample name3T3-L1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.13–-1.20 g/mL
Molecules identified in the studyProtein
Methods used in the studyImmunoelectron microscopy
Western blotting
Mass spectrometry [LCQ-DECA XP]
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2
Experiment ID59
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
PubMed ID 17700640   
OrganismMus musculus
Experiment descriptionCD24 is a marker of exosomes secreted into urine and amniotic fluid.
AuthorsKeller S, Rupp C, Stoeck A, Runz S, Fogel M, Lugert S, Hager HD, Abdel-Bakky MS, Gutwein P, Altevogt P
Journal name KI
Publication year2007
SampleAmniotic fluid
Sample nameAmniotic fluid
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
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3
Experiment ID26
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
PubMed ID 19109410   
OrganismMus musculus
Experiment descriptionProteomic and immunologic analyses of brain tumor exosomes.
AuthorsGraner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD
Journal name FASEB
Publication year2008
SampleBrain cancer cells
Sample nameSMA560vIII
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density1.13-1.15 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [MALDI TOF/TOF]
Western blotting
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4
Experiment ID1052
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 29115712   
OrganismMus musculus
Experiment descriptionProteomic Profiling of Exosomes Secreted by Breast Cancer Cells with Varying Metastatic Potential.
AuthorsGangoda L, Liem M, Ang CS, Keerthikumar S, Adda CG, Parker BS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleBreast cancer cells
Sample name66cl4
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
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5
Experiment ID1051
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
Western blotting
PubMed ID 29115712   
OrganismMus musculus
Experiment descriptionProteomic Profiling of Exosomes Secreted by Breast Cancer Cells with Varying Metastatic Potential.
AuthorsGangoda L, Liem M, Ang CS, Keerthikumar S, Adda CG, Parker BS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleBreast cancer cells
Sample name67NR
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
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6
Experiment ID1053
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
Western blotting
PubMed ID 29115712   
OrganismMus musculus
Experiment descriptionProteomic Profiling of Exosomes Secreted by Breast Cancer Cells with Varying Metastatic Potential.
AuthorsGangoda L, Liem M, Ang CS, Keerthikumar S, Adda CG, Parker BS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleBreast cancer cells
Sample name4T1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
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7
Experiment ID1054
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
Western blotting
PubMed ID 29115712   
OrganismMus musculus
Experiment descriptionProteomic Profiling of Exosomes Secreted by Breast Cancer Cells with Varying Metastatic Potential.
AuthorsGangoda L, Liem M, Ang CS, Keerthikumar S, Adda CG, Parker BS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleBreast cancer cells
Sample name4T1.2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
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8
Experiment ID3
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
Western blotting
PubMed ID 10545503   
OrganismMus musculus
Experiment descriptionMolecular characterization of dendritic cell-derived exosomes. Selective accumulation of the heat shock protein hsc73.
AuthorsThery C, Regnault A, Garin J, Wolfers J, Zitvogel L, Ricciardi-Castagnoli P, Raposo G, Amigorena S.
Journal name JCB
Publication year1999
SampleDendritic cells
Sample nameD1
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density1.14-1.20 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [MALDI TOF]
Western blotting
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9
Experiment ID10
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
PubMed ID 11390481   
OrganismMus musculus
Experiment descriptionProteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles.
AuthorsThery C, Boussac M, Vén P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S.
Journal name JIMMU
Publication year2001
SampleDendritic cells
Sample nameD1
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density1.15-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
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10
Experiment ID73
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 18494037   
OrganismMus musculus
Experiment descriptionDifference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
AuthorsJi H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year2008
SampleEmbryonic fibroblasts
Sample nameNIH3T3
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LCQ DECA]
Western blotting
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11
Experiment ID1000
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 24079734   
OrganismMus musculus
Experiment descriptionImmunostimulatory activity of murine keratinocyte-derived exosomes.
AuthorsKotzerke K, Mempel M, Aung T, Wulf GG, Urlaub H, Wenzel D, SchöP, Braun A.
Journal name Exp Dermatol.
Publication year2013
SampleKeratinocytes
Sample nameMPEK-BL6
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV130042: EV-METRIC:56%
12
Experiment ID1001
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 24079734   
OrganismMus musculus
Experiment descriptionImmunostimulatory activity of murine keratinocyte-derived exosomes.
AuthorsKotzerke K, Mempel M, Aung T, Wulf GG, Urlaub H, Wenzel D, SchöP, Braun A.
Journal name Exp Dermatol.
Publication year2013
SampleKeratinocytes
Sample nameMPEK-BL6 - treated with IFN-.
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV130042: EV-METRIC:56%
13
Experiment ID523
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23658846   
OrganismMus musculus
Experiment descriptionImmunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
AuthorsHassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year2013
SampleMacrophages
Sample nameLeishmania-infected-Macrophage (J774A.1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130144: EV-METRIC:0%, 33%
14
Experiment ID524
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23658846   
OrganismMus musculus
Experiment descriptionImmunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
AuthorsHassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year2013
SampleMacrophages
Sample nameLPS-treated-Macrophage (J774A.1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130144: EV-METRIC:0%, 33%
15
Experiment ID525
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23658846   
OrganismMus musculus
Experiment descriptionImmunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
AuthorsHassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year2013
SampleMacrophages
Sample nameNormal-Macrophage (J774A.1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130144: EV-METRIC:0%, 33%
16
Experiment ID1168
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 29907695   
OrganismMus musculus
Experiment descriptionCancer cells copy migratory behavior and exchange signaling networks via extracellular vesicles.
AuthorsSteenbeek SC, Pham TV, de Ligt J, Zomer A, Knol JC, Piersma SR, Schelfhorst T, Huisjes R, Schiffelers RM, Cuppen E, Jimenez CR, van Rheenen J.
Journal name EMBO J.
Publication year2018
SampleMelanoma cells
Sample name B16F10 (16.5 K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyMass spectrometry
RNA sequencing
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17
Experiment ID1169
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 29907695   
OrganismMus musculus
Experiment descriptionCancer cells copy migratory behavior and exchange signaling networks via extracellular vesicles.
AuthorsSteenbeek SC, Pham TV, de Ligt J, Zomer A, Knol JC, Piersma SR, Schelfhorst T, Huisjes R, Schiffelers RM, Cuppen E, Jimenez CR, van Rheenen J.
Journal name EMBO J.
Publication year2018
SampleMelanoma cells
Sample nameB16F1 (100 K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyMass spectrometry
RNA sequencing
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18
Experiment ID1170
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 29907695   
OrganismMus musculus
Experiment descriptionCancer cells copy migratory behavior and exchange signaling networks via extracellular vesicles.
AuthorsSteenbeek SC, Pham TV, de Ligt J, Zomer A, Knol JC, Piersma SR, Schelfhorst T, Huisjes R, Schiffelers RM, Cuppen E, Jimenez CR, van Rheenen J.
Journal name EMBO J.
Publication year2018
SampleMelanoma cells
Sample name B16F10 (100 K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyMass spectrometry
RNA sequencing
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19
Experiment ID14
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
Western blotting
PubMed ID 16081791   
OrganismMus musculus
Experiment descriptionProteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
AuthorsPotolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L
Journal name JIMMU
Publication year2005
SampleMicroglia
Sample nameN9
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density1.15 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LCQ DECA XP]
Western blotting
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20
Experiment ID1055
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
Western blotting
PubMed ID 30035390   
OrganismMus musculus
Experiment descriptionArrestin-domain Containing Protein 1 (Arrdc1) Regulates the Protein Cargo and Release of Extracellular Vesicles.
AuthorsAnand S, Foot N, Ang CS, Gembus KM, Keerthikumar S, Adda CG, Mathivanan S, Kumar S.
Journal name Proteomics.
Publication year2018
SampleMouse embryonic fibroblasts
Sample nameMEFs - WT 10K
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
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21
Experiment ID1056
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
Western blotting
PubMed ID 30035390   
OrganismMus musculus
Experiment descriptionArrestin-domain Containing Protein 1 (Arrdc1) Regulates the Protein Cargo and Release of Extracellular Vesicles.
AuthorsAnand S, Foot N, Ang CS, Gembus KM, Keerthikumar S, Adda CG, Mathivanan S, Kumar S.
Journal name Proteomics.
Publication year2018
SampleMouse embryonic fibroblasts
Sample nameMEFs - WT exosomes
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
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22
Experiment ID1057
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
Western blotting
PubMed ID 30035390   
OrganismMus musculus
Experiment descriptionArrestin-domain Containing Protein 1 (Arrdc1) Regulates the Protein Cargo and Release of Extracellular Vesicles.
AuthorsAnand S, Foot N, Ang CS, Gembus KM, Keerthikumar S, Adda CG, Mathivanan S, Kumar S.
Journal name Proteomics.
Publication year2018
SampleMouse embryonic fibroblasts
Sample nameMEFs - Arrdc1 KO 10K
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
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23
Experiment ID1058
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
Western blotting
PubMed ID 30035390   
OrganismMus musculus
Experiment descriptionArrestin-domain Containing Protein 1 (Arrdc1) Regulates the Protein Cargo and Release of Extracellular Vesicles.
AuthorsAnand S, Foot N, Ang CS, Gembus KM, Keerthikumar S, Adda CG, Mathivanan S, Kumar S.
Journal name Proteomics.
Publication year2018
SampleMouse embryonic fibroblasts
Sample nameMEFs - Arrdc1 KO exosomes
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
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24
Experiment ID996
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 24392111   
OrganismMus musculus
Experiment descriptionProteomic analysis of C2C12 myoblast and myotube exosome-like vesicles: a new paradigm for myoblast-myotube cross talk?
AuthorsForterre A, Jalabert A, Berger E, Baudet M, Chikh K, Errazuriz E, De Larichaudy J, Chanon S, Weiss-Gayet M, Hesse AM, Record M, Geloen A, Lefai E, Vidal H, Couté, Rome S.
Journal name PLoS One.
Publication year2014
SampleMurine myoblast
Sample nameC2C12 - MB
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV140002: EV-METRIC:67%
25
Experiment ID997
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 24392111   
OrganismMus musculus
Experiment descriptionProteomic analysis of C2C12 myoblast and myotube exosome-like vesicles: a new paradigm for myoblast-myotube cross talk?
AuthorsForterre A, Jalabert A, Berger E, Baudet M, Chikh K, Errazuriz E, De Larichaudy J, Chanon S, Weiss-Gayet M, Hesse AM, Record M, Geloen A, Lefai E, Vidal H, Couté, Rome S.
Journal name PLoS One.
Publication year2014
SampleMurine myotubues
Sample nameC2C12 - MT
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV140002: EV-METRIC:67%
26
Experiment ID675
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method FACS
PubMed ID 29951053   
OrganismMus musculus
Experiment descriptionImmunoregulatory Effects of Myeloid-Derived Suppressor Cell Exosomes in Mouse Model of Autoimmune Alopecia Areata.
AuthorsZör M, Zhao K, Kutlu N, Bauer N, Provaznik J, Hackert T, Schnör M.
Journal name Front Immunol
Publication year2018
SampleMyeloid-derived suppressor cells
Sample nameIn-vitro- Alopecia areata affected C3H/HeJ mice BMC derived
Isolation/purification methodsUltracentrifugation
Density gradient centrifugation
Flotation densityNot provided
Molecules identified in the studyProtein
Methods used in the studyFACS
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27
Experiment ID372
Identified moleculeprotein
Extracellular vesicle typeMatrix vesicles
Identification method Mass spectrometry
PubMed ID 17096383   
OrganismMus musculus
Experiment descriptionAnalysis of the extracellular matrix vesicle proteome in mineralizing osteoblasts.
AuthorsXiao Z, Camalier CE, Nagashima K, Chan KC, Lucas DA, de la Cruz MJ, Gignac M, Lockett S, Issaq HJ, Veenstra TD, Conrads TP, Beck GR Jr.
Journal name J Cell Physiol
Publication year2007
SampleOsteoblasts
Sample nameMC3T3-E1
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
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28
Experiment ID60
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
PubMed ID 17700640   
OrganismMus musculus
Experiment descriptionCD24 is a marker of exosomes secreted into urine and amniotic fluid.
AuthorsKeller S, Rupp C, Stoeck A, Runz S, Fogel M, Lugert S, Hager HD, Abdel-Bakky MS, Gutwein P, Altevogt P
Journal name KI
Publication year2007
SampleUrine
Sample nameUrine - Normal
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -