Gene description for CLDN4
Gene name claudin 4
Gene symbol CLDN4
Other names/aliases CPE-R
CPER
CPETR
CPETR1
WBSCR8
hCPE-R
Species Homo sapiens
 Database cross references - CLDN4
Vesiclepedia VP_1364
ExoCarta ExoCarta_1364
Entrez Gene 1364
HGNC 2046
MIM 602909
 CLDN4 identified in extracellular vesicles derived from the following tissue/cell type
Ascites [Microvesicles]      More >>> 21630462   
Brain cancer cells [Extracellular vesicles]      More >>> 27894104   
Brain cancer cells [Extracellular vesicles]      More >>> 27894104   
Breast cancer cells [Extracellular vesicles]      More >>> 27894104   
Breast cancer cells [Extracellular vesicles]      More >>> 27894104   
Colorectal cancer cells [Exosomes]      More >>> 19837982   
Colorectal cancer cells [Microvesicles]      More >>> 19930720   
Colorectal cancer cells [Exosomes]      More >>> 22285593   
Colorectal cancer cells [Exosomes]      More >>> 22285593   
Colorectal cancer cells [Exosomes]      More >>> 22285593   
Colorectal cancer cells [Exosomes]      More >>> 23161513   
Colorectal cancer cells [Exosomes]      More >>> 23161513   
Colorectal cancer cells [Exosomes]      More >>> 23161513   
Colorectal cancer cells [Exosomes/Extracellular vesicles/Microvesicles]      More >>> 23230278   
Colorectal cancer cells [Exosomes/Extracellular vesicles/Microvesicles]      More >>> 23230278   
Colorectal cancer cells [Exosomes/Extracellular vesicles/Microvesicles]      More >>> 23230278   
Colorectal cancer cells [Exosomes/Membrane vesicles]      More >>> 23585443   
Colorectal cancer cells [Exosomes/Membrane vesicles]      More >>> 23585443   
Colorectal cancer cells [Extracellular vesicles]      More >>> 28842968   
Kidney cells [Exosomes/Extracellular vesicles]      More >>> 23645497   
Kidney cells [Exosomes/Extracellular vesicles]      More >>> 23645497   
Lung cancer cells [Extracellular vesicles]      More >>> 27894104   
Lung cancer cells [Extracellular vesicles]      More >>> 24946052   
Lung cancer cells [Extracellular vesicles]      More >>> 24946052   
Lung cancer cells [Extracellular vesicles]      More >>> 24946052   
Malignant pleural effusions [Exosomes/Microvesicles/Ectosomes/Microparticles]      More >>> 23585444   
Melanoma cells [Extracellular vesicles]      More >>> 27894104   
Melanoma cells [Extracellular vesicles]      More >>> 27894104   
Melanoma cells [Extracellular vesicles]      More >>> 27894104   
Melanoma cells [Extracellular vesicles]      More >>> 27894104   
Ovarian cancer cells [Exosomes]      More >>> 19619303   
Ovarian cancer cells [Extracellular vesicles]      More >>> 27894104   
Ovarian cancer cells [Extracellular vesicles]      More >>> 27894104   
Ovarian cancer cells [Exosomes]      More >>> 24434149   
Ovarian cancer cells [Exosomes]      More >>> 24434149   
Plasma [Exosomes]      More >>> 19619303   
Prostate cancer cells [Extracellular vesicles]      More >>> 28881726   
Prostate cancer cells [Extracellular vesicles]      More >>> 28881726   
Prostate cancer cells [Extracellular vesicles]      More >>> 28881726   
Prostate cancer cells [Extracellular vesicles]      More >>> 28881726   
Serum [Exosomes]      More >>> 22808001   
Serum [Exosomes]      More >>> 22808001   
 Experiment description of studies that identified CLDN4 in extracellular vesicles
1
Experiment ID157
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 21630462   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of microvesicles derived from human colorectal cancer ascites
AuthorsChoi DS, Park JO, Jang SC, Yoon YJ, Jung JW, Choi DY, Kim JW, Kang JS, Park J, Hwang D, Lee KH, Park SH, Kim YK, Desiderio DM, Kim KP, Gho YS
Journal name Proteomics
Publication year2011
SampleAscites
Sample nameMalignant ascites - Colorectal cancer patient 1
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
OptiPrep density gradient
Flotation density1.09 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK EV110017: EV-METRIC:38%
2
Experiment ID580
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBrain cancer cells
Sample nameT-47D
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
3
Experiment ID586
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBrain cancer cells
Sample nameU251
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
4
Experiment ID576
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBreast cancer cells
Sample nameHS 578T
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
5
Experiment ID579
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBreast cancer cells
Sample nameMDA-MB-468
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
6
Experiment ID21
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method
Mass spectrometry   Supporting data   MS    
PubMed ID 19837982   
OrganismHomo sapiens
Experiment descriptionProteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
AuthorsSuresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson
Journal name MCP
Publication year2009
SampleColorectal cancer cells
Sample nameLIM1215
Isolation/purification methodsFiltration
Ultracentrifugation
Sucrose density gradient
Flotation density1.10-1.12 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV100013: EV-METRIC:25%, 56%
7
Experiment ID303
Identified moleculemrna
Extracellular vesicle typeMicrovesicles
Identification method Microarray [Illumina]
PubMed ID 19930720   
OrganismHomo sapiens
Experiment descriptionColorectal cancer cell-derived microvesicles are enriched in cell cycle-related mRNAs that promote proliferation of endothelial cells
AuthorsHong BS, Cho JH, Kim H, Choi EJ, Rho S, Kim J, Kim JH, Choi DS, Kim YK, Hwang D, Gho YS
Journal name BMC Genomics
Publication year2009
SampleColorectal cancer cells
Sample nameSW480
Isolation/purification methodsDifferential centrifugation
Ultrafiltration
OptiPrep density gradient
Flotation density1.09 g/mL
Molecules identified in the studyProtein
mRNA
Methods used in the studyWestern blotting
RT-PCR
Microarray [Illumina]
EV-TRACK -
8
Experiment ID347
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 22285593   
OrganismHomo sapiens
Experiment descriptionComparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes.
AuthorsTauro BJ, Greening DW, Mathias RA, Ji H, Mathivanan S, Scott AM, Simpson RJ
Journal name Methods
Publication year2012
SampleColorectal cancer cells
Sample nameLIM1863 - Ultracentrifugation
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV120024: EV-METRIC:25%, 33%, 50%
9
Experiment ID349
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 22285593   
OrganismHomo sapiens
Experiment descriptionComparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes.
AuthorsTauro BJ, Greening DW, Mathias RA, Ji H, Mathivanan S, Scott AM, Simpson RJ
Journal name Methods
Publication year2012
SampleColorectal cancer cells
Sample nameLIM1863 - OptiPrep density gradient
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV120024: EV-METRIC:25%, 33%, 50%
10
Experiment ID348
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 22285593   
OrganismHomo sapiens
Experiment descriptionComparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes.
AuthorsTauro BJ, Greening DW, Mathias RA, Ji H, Mathivanan S, Scott AM, Simpson RJ
Journal name Methods
Publication year2012
SampleColorectal cancer cells
Sample nameLIM1863 - EpCAM immunoaffinity
Isolation/purification methodsDifferential centrifugation
Filtration
Immunoaffinity (EpCAM)
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV120024: EV-METRIC:25%, 33%, 50%
11
Experiment ID480
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23161513   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
AuthorsDemory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year2012
SampleColorectal cancer cells
Sample nameDKO-1
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130004: EV-METRIC:44%
12
Experiment ID481
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23161513   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
AuthorsDemory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year2012
SampleColorectal cancer cells
Sample nameDks-8
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130004: EV-METRIC:44%
13
Experiment ID482
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23161513   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
AuthorsDemory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year2012
SampleColorectal cancer cells
Sample nameDLD-1
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130004: EV-METRIC:44%
14
Experiment ID486
Identified moleculeprotein
Extracellular vesicle typeExosomes/Extracellular vesicles/Microvesicles
Identification method Mass spectrometry
PubMed ID 23230278   
OrganismHomo sapiens
Experiment descriptionTwo distinct populations of exosomes are released from LIM1863 colon carcinoma cell-derived organoids.
AuthorsTauro BJ, Greening DW, Mathias RA, Mathivanan S, Ji H, Simpson RJ.
Journal name Mol Cell Proteomics
Publication year2012
SampleColorectal cancer cells
Sample nameA33 affinity purified exosomes-Colorectal cancer cells (LIM1863)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130020: EV-METRIC:38%
15
Experiment ID487
Identified moleculeprotein
Extracellular vesicle typeExosomes/Extracellular vesicles/Microvesicles
Identification method Mass spectrometry
PubMed ID 23230278   
OrganismHomo sapiens
Experiment descriptionTwo distinct populations of exosomes are released from LIM1863 colon carcinoma cell-derived organoids.
AuthorsTauro BJ, Greening DW, Mathias RA, Mathivanan S, Ji H, Simpson RJ.
Journal name Mol Cell Proteomics
Publication year2012
SampleColorectal cancer cells
Sample nameEpCAM affinity purified exosomes-Colorectal cancer cells (LIM1863)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130020: EV-METRIC:38%
16
Experiment ID488
Identified moleculeprotein
Extracellular vesicle typeExosomes/Extracellular vesicles/Microvesicles
Identification method Mass spectrometry
PubMed ID 23230278   
OrganismHomo sapiens
Experiment descriptionTwo distinct populations of exosomes are released from LIM1863 colon carcinoma cell-derived organoids.
AuthorsTauro BJ, Greening DW, Mathias RA, Mathivanan S, Ji H, Simpson RJ.
Journal name Mol Cell Proteomics
Publication year2012
SampleColorectal cancer cells
Sample nameShed vesicles-Colorectal cancer cells (LIM1863)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130020: EV-METRIC:38%
17
Experiment ID517
Identified moleculeprotein
Extracellular vesicle typeExosomes/Membrane vesicles
Identification method Mass spectrometry
PubMed ID 23585443   
OrganismHomo sapiens
Experiment descriptionProteome profiling of exosomes derived from human primary and metastatic colorectal cells reveal differential expression of key metastatic factors and signal transduction components.
AuthorsJi H, Greening DW, Barnes TW, Lim JW, Tauro BJ, Rai A, Xu R, Adda C, Mathivanan S, Zhao W, Xue Y, Xu T, Zhu HJ, Simpson RJ.
Journal name Proteomics
Publication year2013
SampleColorectal cancer cells
Sample nameSW480
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130010: EV-METRIC:44%
18
Experiment ID518
Identified moleculeprotein
Extracellular vesicle typeExosomes/Membrane vesicles
Identification method Mass spectrometry
PubMed ID 23585443   
OrganismHomo sapiens
Experiment descriptionProteome profiling of exosomes derived from human primary and metastatic colorectal cells reveal differential expression of key metastatic factors and signal transduction components.
AuthorsJi H, Greening DW, Barnes TW, Lim JW, Tauro BJ, Rai A, Xu R, Adda C, Mathivanan S, Zhao W, Xue Y, Xu T, Zhu HJ, Simpson RJ.
Journal name Proteomics
Publication year2013
SampleColorectal cancer cells
Sample nameSW620
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130010: EV-METRIC:44%
19
Experiment ID1050
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 28842968   
OrganismHomo sapiens
Experiment descriptionInsulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles.
AuthorsLiem M, Ang CS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleColorectal cancer cells
Sample namelim1215 - II
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV170021: EV-METRIC:44%, 44%
20
Experiment ID521
Identified moleculeprotein
Extracellular vesicle typeExosomes/Extracellular vesicles
Identification method Mass spectrometry
PubMed ID 23645497   
OrganismCanis lupus familiaris
Experiment descriptionOncogenic H-Ras reprograms Madin-Darby canine kidney (MDCK) cell-derived exosomal proteins following epithelial-mesenchymal transition.
AuthorsTauro BJ, Mathias RA, Greening DW, Gopal SK, Ji H, Kapp EA, Coleman BM, Hill AF, Kusebauch U, Hallows JL, Shteynberg D, Moritz RL, Zhu HJ, Simpson RJ.
Journal name Mol Cell Proteomics
Publication year2013
SampleKidney cells
Sample nameNormal-Madin-Darby canine kidney cell (MDCK)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130021: EV-METRIC:50%
21
Experiment ID522
Identified moleculeprotein
Extracellular vesicle typeExosomes/Extracellular vesicles
Identification method Mass spectrometry
PubMed ID 23645497   
OrganismCanis lupus familiaris
Experiment descriptionOncogenic H-Ras reprograms Madin-Darby canine kidney (MDCK) cell-derived exosomal proteins following epithelial-mesenchymal transition.
AuthorsTauro BJ, Mathias RA, Greening DW, Gopal SK, Ji H, Kapp EA, Coleman BM, Hill AF, Kusebauch U, Hallows JL, Shteynberg D, Moritz RL, Zhu HJ, Simpson RJ.
Journal name Mol Cell Proteomics
Publication year2013
SampleKidney cells
Sample nameRas-transformed MDCK cells (21D1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130021: EV-METRIC:50%
22
Experiment ID614
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleLung cancer cells
Sample nameNCI-H322M
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
23
Experiment ID889
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 24946052   
OrganismHomo sapiens
Experiment descriptionExtracellular vesicles shed from gefitinib-resistant nonsmall cell lung cancer regulate the tumor microenvironment
AuthorsChoi DY, You S, Jung JH, Lee JC, Rho JK, Lee KY, Freeman MR, Kim KP, Kim J.
Journal name Proteomics
Publication year2014
SampleLung cancer cells
Sample namePC9 - Rep 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation densityNot provided
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140109: EV-METRIC:25%
24
Experiment ID890
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 24946052   
OrganismHomo sapiens
Experiment descriptionExtracellular vesicles shed from gefitinib-resistant nonsmall cell lung cancer regulate the tumor microenvironment
AuthorsChoi DY, You S, Jung JH, Lee JC, Rho JK, Lee KY, Freeman MR, Kim KP, Kim J.
Journal name Proteomics
Publication year2014
SampleLung cancer cells
Sample namePC9 - Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation densityNot provided
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140109: EV-METRIC:25%
25
Experiment ID891
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 24946052   
OrganismHomo sapiens
Experiment descriptionExtracellular vesicles shed from gefitinib-resistant nonsmall cell lung cancer regulate the tumor microenvironment
AuthorsChoi DY, You S, Jung JH, Lee JC, Rho JK, Lee KY, Freeman MR, Kim KP, Kim J.
Journal name Proteomics
Publication year2014
SampleLung cancer cells
Sample namePC9 - Rep 3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation densityNot provided
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140109: EV-METRIC:25%
26
Experiment ID519
Identified moleculeprotein
Extracellular vesicle typeExosomes/Microvesicles/Ectosomes/Microparticles
Identification method Mass spectrometry
PubMed ID 23585444   
OrganismHomo sapiens
Experiment descriptionIdentification and characterization of proteins isolated from microvesicles derived from human lung cancer pleural effusions.
AuthorsPark JO, Choi DY, Choi DS, Kim HJ, Kang JW, Jung JH, Lee JH, Kim J, Freeman MR, Lee KY, Gho YS, Kim KP.
Journal name Proteomics
Publication year2013
SampleMalignant pleural effusions
Sample nameNon-small cell lung cancer patient-Lung cancer pleural effusion
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130049: EV-METRIC:38%
27
Experiment ID617
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleMelanoma cells
Sample nameLOX IMVI
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
28
Experiment ID619
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleMelanoma cells
Sample nameMALME-3M
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
29
Experiment ID620
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleMelanoma cells
Sample nameMDA-MB-435
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
30
Experiment ID621
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleMelanoma cells
Sample nameSK-MEL-2
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
31
Experiment ID67
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
Immunoelectron Microscopy
PubMed ID 19619303   
OrganismHomo sapiens
Experiment descriptionClaudin-containing exosomes in the peripheral circulation of women with ovarian cancer.
AuthorsLi J, Sherman-Baust CA, Tsai-Turton M, Bristow RE, Roden RB, Morin PJ
Journal name BMC_CANCER
Publication year2009
SampleOvarian cancer cells
Sample nameBG-1
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density1.15-1.21 g/mL
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Immunoelectron microscopy
EV-TRACK -
32
Experiment ID627
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleOvarian cancer cells
Sample nameOVCAR-3
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
33
Experiment ID632
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleOvarian cancer cells
Sample nameNCI-ADR-RES
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
34
Experiment ID992
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 24434149   
OrganismHomo sapiens
Experiment descriptionIn-depth proteomic analyses of ovarian cancer cell line exosomes reveals differential enrichment of functional categories compared to the NCI 60 proteome.
AuthorsSinha A, Ignatchenko V, Ignatchenko A, Mejia-Guerrero S, Kislinger T.
Journal name Biochem Biophys Res Commun.
Publication year2014
SampleOvarian cancer cells
Sample nameOVCAR3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV140099: EV-METRIC:44%
35
Experiment ID993
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 24434149   
OrganismHomo sapiens
Experiment descriptionIn-depth proteomic analyses of ovarian cancer cell line exosomes reveals differential enrichment of functional categories compared to the NCI 60 proteome.
AuthorsSinha A, Ignatchenko V, Ignatchenko A, Mejia-Guerrero S, Kislinger T.
Journal name Biochem Biophys Res Commun.
Publication year2014
SampleOvarian cancer cells
Sample nameOVCAR433
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV140099: EV-METRIC:44%
36
Experiment ID68
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
PubMed ID 19619303   
OrganismHomo sapiens
Experiment descriptionClaudin-containing exosomes in the peripheral circulation of women with ovarian cancer.
AuthorsLi J, Sherman-Baust CA, Tsai-Turton M, Bristow RE, Roden RB, Morin PJ
Journal name BMC_CANCER
Publication year2009
SamplePlasma
Sample namePlasma - Ovarian cancer
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -
37
Experiment ID843
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 28881726   
OrganismHomo sapiens
Experiment descriptionModulation of paracrine signaling by CD9 positive small extracellular vesicles mediates cellular growth of androgen deprived prostate cancer
AuthorsSoekmadji C, Riches JD, Russell PJ, Ruelcke JE, McPherson S, Wang C, Hovens CM, Corcoran NM; Australian Prostate Cancer Collaboration BioResource, Hill MM, Nelson CC.
Journal name Oncotarget
Publication year2016
SampleProstate cancer cells
Sample nameLNCaP - in CSS - Rep 3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170047: EV-METRIC:44%, 0%, 44%, 44%, 44%, 33%, 33%, 33%
38
Experiment ID847
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 28881726   
OrganismHomo sapiens
Experiment descriptionModulation of paracrine signaling by CD9 positive small extracellular vesicles mediates cellular growth of androgen deprived prostate cancer
AuthorsSoekmadji C, Riches JD, Russell PJ, Ruelcke JE, McPherson S, Wang C, Hovens CM, Corcoran NM; Australian Prostate Cancer Collaboration BioResource, Hill MM, Nelson CC.
Journal name Oncotarget
Publication year2016
SampleProstate cancer cells
Sample nameLNCaP - in CSS with DHT treatment - Rep 3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170047: EV-METRIC:44%, 0%, 44%, 44%, 44%, 33%, 33%, 33%
39
Experiment ID856
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 28881726   
OrganismHomo sapiens
Experiment descriptionModulation of paracrine signaling by CD9 positive small extracellular vesicles mediates cellular growth of androgen deprived prostate cancer
AuthorsSoekmadji C, Riches JD, Russell PJ, Ruelcke JE, McPherson S, Wang C, Hovens CM, Corcoran NM; Australian Prostate Cancer Collaboration BioResource, Hill MM, Nelson CC.
Journal name Oncotarget
Publication year2016
SampleProstate cancer cells
Sample nameDUCaP - in CSS with DHT treatment - Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170047: EV-METRIC:44%, 0%, 44%, 44%, 44%, 33%, 33%, 33%
40
Experiment ID857
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 28881726   
OrganismHomo sapiens
Experiment descriptionModulation of paracrine signaling by CD9 positive small extracellular vesicles mediates cellular growth of androgen deprived prostate cancer
AuthorsSoekmadji C, Riches JD, Russell PJ, Ruelcke JE, McPherson S, Wang C, Hovens CM, Corcoran NM; Australian Prostate Cancer Collaboration BioResource, Hill MM, Nelson CC.
Journal name Oncotarget
Publication year2016
SampleProstate cancer cells
Sample nameDUCaP - in CSS with DHT treatment - Rep 3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170047: EV-METRIC:44%, 0%, 44%, 44%, 44%, 33%, 33%, 33%
41
Experiment ID971
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 22808001   
OrganismHomo sapiens
Experiment descriptionBiochemical and physical characterisation of urinary nanovesicles following CHAPS treatment.
AuthorsMusante L, Saraswat M, Duriez E, Byrne B, Ravidà, Domon B, Holthofer H.
Journal name PLoS One
Publication year2012
SampleSerum
Sample nameSerum -Tuberculosis patient 1
Isolation/purification methodsFiltration
ExoQuick
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120006: EV-METRIC:56%
42
Experiment ID978
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 22808001   
OrganismHomo sapiens
Experiment descriptionBiochemical and physical characterisation of urinary nanovesicles following CHAPS treatment.
AuthorsMusante L, Saraswat M, Duriez E, Byrne B, Ravidà, Domon B, Holthofer H.
Journal name PLoS One
Publication year2012
SampleSerum
Sample nameSerum -Tuberculosis patient 8
Isolation/purification methodsFiltration
ExoQuick
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120006: EV-METRIC:56%