Gene description for Hspa8
Gene name heat shock protein 8
Gene symbol Hspa8
Other names/aliases 2410008N15Rik
Hsc70
Hsc71
Hsc73
Hsp73
Hspa10
Species Mus musculus
 Database cross references - Hspa8
Vesiclepedia VP_15481
ExoCarta ExoCarta_15481
Entrez Gene 15481
 Hspa8 identified in extracellular vesicles derived from the following tissue/cell type
adipocytes [Exosomes]      More >>> 24513287   
B cells [Exosomes]      More >>> 12626558   
Basophilic leukemia cells [Exosomes]      More >>> 20424270   
Basophilic leukemia cells [Exosomes]      More >>> 20424270   
Brain cancer cells [Exosomes]      More >>> 19109410   
Breast cancer cells [Exosomes]      More >>> 25220352   
Breast cancer cells [Exosomes]      More >>> 29115712   
Breast cancer cells [Exosomes]      More >>> 29115712   
Breast cancer cells [Exosomes]      More >>> 29115712   
Breast cancer cells [Exosomes]      More >>> 29115712   
Dendritic cells [Exosomes]      More >>> 10545503   
Embryonic fibroblasts [Exosomes]      More >>> 18494037   
Fibroblasts [Exosomes]      More >>> 23260141   
Insulinoma cells [Microparticles]      More >>> 22345510   
Insulinoma cells [Microparticles]      More >>> 22345510   
Insulinoma cells [Microparticles]      More >>> 22345510   
Insulinoma cells [Exosomes]      More >>> 22345510   
Insulinoma cells [Exosomes]      More >>> 22345510   
Insulinoma cells [Exosomes]      More >>> 22345510   
Insulinoma cells [Exosomes]      More >>> 21734072   
Insulinoma cells [Exosomes]      More >>> 21734072   
Macrophages [Exosomes]      More >>> 12626558   
Macrophages [Exosomes]      More >>> 23658846   
Macrophages [Exosomes]      More >>> 23658846   
Macrophages [Exosomes]      More >>> 23658846   
Mast cells [Exosomes]      More >>> 12626558   
Mast cells [Exosomes]      More >>> 17486113   
Mast cells [Exosomes/Nanovesicles]      More >>> 21179422   
Mast cells [Exosomes/Nanovesicles]      More >>> 21179422   
Melanoma cells [Extracellular vesicles]      More >>> 29907695   
Melanoma cells [Extracellular vesicles]      More >>> 29907695   
Microglia [Exosomes]      More >>> 16081791   
Mouse embryonic fibroblasts [Extracellular vesicles]      More >>> 30035390   
Mouse embryonic fibroblasts [Extracellular vesicles]      More >>> 30035390   
Mouse embryonic fibroblasts [Extracellular vesicles]      More >>> 30035390   
Mouse embryonic fibroblasts [Extracellular vesicles]      More >>> 30035390   
Mouse myoblast [Microvesicles]      More >>> 20399774   
Mov neuroglial cell [Exosomes]      More >>> 15210972   
Murine myoblast [Extracellular vesicles]      More >>> 24392111   
Murine myotubues [Extracellular vesicles]      More >>> 24392111   
Myeloid-derived suppressor cells [Exosomes]      More >>> 29951053   
Myeloid-derived suppressor cells [Exosomes]      More >>> 29951053   
Myeloid-derived suppressor cells [Exosomes]      More >>> 24295599   
Myeloid-derived suppressor cells [Exosomes]      More >>> 24295599   
Oligodendrocytes [Exosomes]      More >>> 21136642   
Oligodendrocytes [Microvesicles]      More >>> 21842121   
Oligodendrocytes [Exosomes]      More >>> 21136642   
Osteoblasts [Matrix vesicles]      More >>> 17096383   
Pancreatic cells [Exosomes]      More >>> 19351151   
T cells [Exosomes]      More >>> 23463506   
Thymus [Microvesicles]      More >>> 21635979   
Thymus [Apoptotic bodies]      More >>> 21635979   
 Experiment description of studies that identified Hspa8 in extracellular vesicles
1
Experiment ID1098
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 24513287   
OrganismMus musculus
Experiment descriptionLipid synthesis is promoted by hypoxic adipocyte-derived exosomes in 3T3-L1 cells.
AuthorsSano S, Izumi Y, Yamaguchi T, Yamazaki T, Tanaka M, Shiota M, Osada-Oka M, Nakamura Y, Wei M, Wanibuchi H, Iwao H, Yoshiyama M.
Journal name Biochem Biophys Res Commun
Publication year2014
Sampleadipocytes
Sample name3T3-L1- Hypoxic conditions
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140281: EV-METRIC:0%, 22%
2
Experiment ID8
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
PubMed ID 12626558   
OrganismMus musculus
Experiment descriptionMast cell-derived exosomes induce phenotypic and functional maturation of dendritic cells and elicit specific immune responses in vivo.
AuthorsSkokos D, Botros HG, Demeure C, Morin J, Peronet R, Birkenmeier G, Boudaly S, Mecheri S.
Journal name JIMMU
Publication year2003
SampleB cells
Sample nameB cells
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -
3
Experiment ID89
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 20424270   
OrganismRattus norvegicus
Experiment descriptionExosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins.
AuthorsSubra C, Grand D, Laulagnier K, Stella A, Lambeau G, Paillasse M, De Medina P, Monsarrat B, Perret B, Silvente-Poirot S, Poirot M, Record M.
Journal name JLR
Publication year2010
SampleBasophilic leukemia cells
Sample nameRBL-2H3
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density-
Molecules identified in the studyProtein
Lipids
Methods used in the studyWestern blotting
Gas chromatography
Thin layer chromatography
Mass spectrometry [Qstar XL]
FACS
EV-TRACK EV100089: EV-METRIC:0%
4
Experiment ID89
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 20424270   
OrganismRattus norvegicus
Experiment descriptionExosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins.
AuthorsSubra C, Grand D, Laulagnier K, Stella A, Lambeau G, Paillasse M, De Medina P, Monsarrat B, Perret B, Silvente-Poirot S, Poirot M, Record M.
Journal name JLR
Publication year2010
SampleBasophilic leukemia cells
Sample nameRBL-2H3
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density-
Molecules identified in the studyProtein
Lipids
Methods used in the studyWestern blotting
Gas chromatography
Thin layer chromatography
Mass spectrometry [Qstar XL]
FACS
EV-TRACK EV100089: EV-METRIC:0%
5
Experiment ID26
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 19109410   
OrganismMus musculus
Experiment descriptionProteomic and immunologic analyses of brain tumor exosomes.
AuthorsGraner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD
Journal name FASEB
Publication year2008
SampleBrain cancer cells
Sample nameSMA560vIII
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density1.13-1.15 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [MALDI TOF/TOF]
Western blotting
EV-TRACK -
6
Experiment ID737
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 25220352   
OrganismMus musculus
Experiment descriptionSurface functionalization of exosomes using click chemistry.
AuthorsSmyth T, Petrova K, Payton NM, Persaud I, Redzic JS, Graner MW, Smith-Jones P, Anchordoquy TJ.
Journal name Bioconjug Chem
Publication year2014
SampleBreast cancer cells
Sample name4T1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.1-1.2 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140392: EV-METRIC:14%
7
Experiment ID1051
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 29115712   
OrganismMus musculus
Experiment descriptionProteomic Profiling of Exosomes Secreted by Breast Cancer Cells with Varying Metastatic Potential.
AuthorsGangoda L, Liem M, Ang CS, Keerthikumar S, Adda CG, Parker BS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleBreast cancer cells
Sample name67NR
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -
8
Experiment ID1052
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 29115712   
OrganismMus musculus
Experiment descriptionProteomic Profiling of Exosomes Secreted by Breast Cancer Cells with Varying Metastatic Potential.
AuthorsGangoda L, Liem M, Ang CS, Keerthikumar S, Adda CG, Parker BS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleBreast cancer cells
Sample name66cl4
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -
9
Experiment ID1053
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 29115712   
OrganismMus musculus
Experiment descriptionProteomic Profiling of Exosomes Secreted by Breast Cancer Cells with Varying Metastatic Potential.
AuthorsGangoda L, Liem M, Ang CS, Keerthikumar S, Adda CG, Parker BS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleBreast cancer cells
Sample name4T1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -
10
Experiment ID1054
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 29115712   
OrganismMus musculus
Experiment descriptionProteomic Profiling of Exosomes Secreted by Breast Cancer Cells with Varying Metastatic Potential.
AuthorsGangoda L, Liem M, Ang CS, Keerthikumar S, Adda CG, Parker BS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleBreast cancer cells
Sample name4T1.2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -
11
Experiment ID3
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
Western blotting
PubMed ID 10545503   
OrganismMus musculus
Experiment descriptionMolecular characterization of dendritic cell-derived exosomes. Selective accumulation of the heat shock protein hsc73.
AuthorsThery C, Regnault A, Garin J, Wolfers J, Zitvogel L, Ricciardi-Castagnoli P, Raposo G, Amigorena S.
Journal name JCB
Publication year1999
SampleDendritic cells
Sample nameD1
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density1.14-1.20 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [MALDI TOF]
Western blotting
EV-TRACK -
12
Experiment ID73
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 18494037   
OrganismMus musculus
Experiment descriptionDifference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
AuthorsJi H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year2008
SampleEmbryonic fibroblasts
Sample nameNIH3T3
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LCQ DECA]
Western blotting
EV-TRACK -
13
Experiment ID489
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23260141   
OrganismMus musculus
Experiment descriptionExosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
AuthorsLuga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year2012
SampleFibroblasts
Sample nameNormal-Fibroblasts (L cells)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120034: EV-METRIC:44%, 38%
14
Experiment ID1279
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 1
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%
15
Experiment ID1280
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 2
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%
16
Experiment ID1281
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 3
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%
17
Experiment ID1282
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%
18
Experiment ID1283
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%
19
Experiment ID1284
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%
20
Experiment ID1019
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
PubMed ID 21734072   
OrganismMus musculus
Experiment descriptionInsulinoma-released exosomes or microparticles are immunostimulatory and can activate autoreactive T cells spontaneously developed in nonobese diabetic mice.
AuthorsSheng H, Hassanali S, Nugent C, Wen L, Hamilton-Williams E, Dias P, Dai YD.
Journal name J Immunol.
Publication year2011
SampleInsulinoma cells
Sample nameMIN6
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV110115: EV-METRIC:11%
21
Experiment ID1020
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
PubMed ID 21734072   
OrganismMus musculus
Experiment descriptionInsulinoma-released exosomes or microparticles are immunostimulatory and can activate autoreactive T cells spontaneously developed in nonobese diabetic mice.
AuthorsSheng H, Hassanali S, Nugent C, Wen L, Hamilton-Williams E, Dias P, Dai YD.
Journal name J Immunol.
Publication year2011
SampleInsulinoma cells
Sample nameNIT-1
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK EV110115: EV-METRIC:11%
22
Experiment ID9
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
PubMed ID 12626558   
OrganismMus musculus
Experiment descriptionMast cell-derived exosomes induce phenotypic and functional maturation of dendritic cells and elicit specific immune responses in vivo.
AuthorsSkokos D, Botros HG, Demeure C, Morin J, Peronet R, Birkenmeier G, Boudaly S, Mecheri S.
Journal name JIMMU
Publication year2003
SampleMacrophages
Sample nameMacrophages
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -
23
Experiment ID523
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23658846   
OrganismMus musculus
Experiment descriptionImmunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
AuthorsHassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year2013
SampleMacrophages
Sample nameLeishmania-infected-Macrophage (J774A.1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130144: EV-METRIC:0%, 33%
24
Experiment ID524
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23658846   
OrganismMus musculus
Experiment descriptionImmunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
AuthorsHassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year2013
SampleMacrophages
Sample nameLPS-treated-Macrophage (J774A.1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130144: EV-METRIC:0%, 33%
25
Experiment ID525
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23658846   
OrganismMus musculus
Experiment descriptionImmunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
AuthorsHassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year2013
SampleMacrophages
Sample nameNormal-Macrophage (J774A.1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130144: EV-METRIC:0%, 33%
26
Experiment ID7
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
PubMed ID 12626558   
OrganismMus musculus
Experiment descriptionMast cell-derived exosomes induce phenotypic and functional maturation of dendritic cells and elicit specific immune responses in vivo.
AuthorsSkokos D, Botros HG, Demeure C, Morin J, Peronet R, Birkenmeier G, Boudaly S, Mecheri S.
Journal name JIMMU
Publication year2003
SampleMast cells
Sample nameBone marrow-derived mast cells
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -
27
Experiment ID15
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 17486113   
OrganismMus musculus
Homo sapiens
Experiment descriptionExosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
AuthorsValadi H, Ekströ, Bossios A, Sjöand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year2007
SampleMast cells
Sample nameMC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methodsFiltration
Ultracentrifugation
Sucrose density gradient
Flotation density1.11-1.21 g/mL
Molecules identified in the studyProtein
mRNA
miRNA
Methods used in the studyMass spectrometry [MALDI TOF]
Western blotting
Microarray
MicroRNA array [miRCURY LNA Array]
EV-TRACK -
28
Experiment ID424
Identified moleculemRNA
Extracellular vesicle typeExosomes/Nanovesicles
Identification method Microarray
PubMed ID 21179422   
OrganismMus musculus
Experiment descriptionExosomes communicate protective messages during oxidative stress; possible role of exosomal shuttle RNA.
AuthorsEldh M, Ekström K, Valadi H, Sjöstrand M, Olsson B, Jernås M, Lötvall J.
Journal name PLoS One
Publication year2010
SampleMast cells
Sample nameMast cells (MC/9)
Isolation/purification methods-
Flotation density-
Molecules identified in the studymRNA
Methods used in the studyMicroarray
EV-TRACK EV100074: EV-METRIC:14%
29
Experiment ID425
Identified moleculemRNA
Extracellular vesicle typeExosomes/Nanovesicles
Identification method Microarray
PubMed ID 21179422   
OrganismMus musculus
Experiment descriptionExosomes communicate protective messages during oxidative stress; possible role of exosomal shuttle RNA.
AuthorsEldh M, Ekström K, Valadi H, Sjöstrand M, Olsson B, Jernås M, Lötvall J.
Journal name PLoS One
Publication year2010
SampleMast cells
Sample nameOxidatively stressed-Mast cells (MC/9)
Isolation/purification methods-
Flotation density-
Molecules identified in the studymRNA
Methods used in the studyMicroarray
EV-TRACK EV100074: EV-METRIC:14%
30
Experiment ID1169
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 29907695   
OrganismMus musculus
Experiment descriptionCancer cells copy migratory behavior and exchange signaling networks via extracellular vesicles.
AuthorsSteenbeek SC, Pham TV, de Ligt J, Zomer A, Knol JC, Piersma SR, Schelfhorst T, Huisjes R, Schiffelers RM, Cuppen E, Jimenez CR, van Rheenen J.
Journal name EMBO J.
Publication year2018
SampleMelanoma cells
Sample nameB16F1 (100 K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyMass spectrometry
RNA sequencing
EV-TRACK -
31
Experiment ID1170
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 29907695   
OrganismMus musculus
Experiment descriptionCancer cells copy migratory behavior and exchange signaling networks via extracellular vesicles.
AuthorsSteenbeek SC, Pham TV, de Ligt J, Zomer A, Knol JC, Piersma SR, Schelfhorst T, Huisjes R, Schiffelers RM, Cuppen E, Jimenez CR, van Rheenen J.
Journal name EMBO J.
Publication year2018
SampleMelanoma cells
Sample name B16F10 (100 K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyMass spectrometry
RNA sequencing
EV-TRACK -
32
Experiment ID14
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 16081791   
OrganismMus musculus
Experiment descriptionProteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
AuthorsPotolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L
Journal name JIMMU
Publication year2005
SampleMicroglia
Sample nameN9
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density1.15 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LCQ DECA XP]
Western blotting
EV-TRACK -
33
Experiment ID1055
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30035390   
OrganismMus musculus
Experiment descriptionArrestin-domain Containing Protein 1 (Arrdc1) Regulates the Protein Cargo and Release of Extracellular Vesicles.
AuthorsAnand S, Foot N, Ang CS, Gembus KM, Keerthikumar S, Adda CG, Mathivanan S, Kumar S.
Journal name Proteomics.
Publication year2018
SampleMouse embryonic fibroblasts
Sample nameMEFs - WT 10K
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -
34
Experiment ID1056
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30035390   
OrganismMus musculus
Experiment descriptionArrestin-domain Containing Protein 1 (Arrdc1) Regulates the Protein Cargo and Release of Extracellular Vesicles.
AuthorsAnand S, Foot N, Ang CS, Gembus KM, Keerthikumar S, Adda CG, Mathivanan S, Kumar S.
Journal name Proteomics.
Publication year2018
SampleMouse embryonic fibroblasts
Sample nameMEFs - WT exosomes
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -
35
Experiment ID1057
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30035390   
OrganismMus musculus
Experiment descriptionArrestin-domain Containing Protein 1 (Arrdc1) Regulates the Protein Cargo and Release of Extracellular Vesicles.
AuthorsAnand S, Foot N, Ang CS, Gembus KM, Keerthikumar S, Adda CG, Mathivanan S, Kumar S.
Journal name Proteomics.
Publication year2018
SampleMouse embryonic fibroblasts
Sample nameMEFs - Arrdc1 KO 10K
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -
36
Experiment ID1058
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30035390   
OrganismMus musculus
Experiment descriptionArrestin-domain Containing Protein 1 (Arrdc1) Regulates the Protein Cargo and Release of Extracellular Vesicles.
AuthorsAnand S, Foot N, Ang CS, Gembus KM, Keerthikumar S, Adda CG, Mathivanan S, Kumar S.
Journal name Proteomics.
Publication year2018
SampleMouse embryonic fibroblasts
Sample nameMEFs - Arrdc1 KO exosomes
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -
37
Experiment ID1288
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry
PubMed ID 20399774   
OrganismMus musculus
Experiment descriptionC2C12 myoblasts release micro-vesicles containing mtDNA and proteins involved in signal transduction.
AuthorsGuescini M, Guidolin D, Vallorani L, Casadei L, Gioacchini AM, Tibollo P, Battistelli M, Falcieri E, Battistin L, Agnati LF, Stocchi V.
Journal name Exp Cell Res.
Publication year2010
SampleMouse myoblast
Sample nameC2C12
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV100004: EV-METRIC:44%
38
Experiment ID39
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
Western blotting
PubMed ID 15210972   
OrganismMus musculus
Experiment descriptionCells release prions in association with exosomes.
AuthorsFevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name PNAS
Publication year2004
SampleMov neuroglial cell
Sample nameMov neuroglial cell
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density1.14 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [QSTAR]
Western blotting
Immunoelectron microscopy
EV-TRACK -
39
Experiment ID996
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 24392111   
OrganismMus musculus
Experiment descriptionProteomic analysis of C2C12 myoblast and myotube exosome-like vesicles: a new paradigm for myoblast-myotube cross talk?
AuthorsForterre A, Jalabert A, Berger E, Baudet M, Chikh K, Errazuriz E, De Larichaudy J, Chanon S, Weiss-Gayet M, Hesse AM, Record M, Geloen A, Lefai E, Vidal H, Couté, Rome S.
Journal name PLoS One.
Publication year2014
SampleMurine myoblast
Sample nameC2C12 - MB
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV140002: EV-METRIC:67%
40
Experiment ID997
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 24392111   
OrganismMus musculus
Experiment descriptionProteomic analysis of C2C12 myoblast and myotube exosome-like vesicles: a new paradigm for myoblast-myotube cross talk?
AuthorsForterre A, Jalabert A, Berger E, Baudet M, Chikh K, Errazuriz E, De Larichaudy J, Chanon S, Weiss-Gayet M, Hesse AM, Record M, Geloen A, Lefai E, Vidal H, Couté, Rome S.
Journal name PLoS One.
Publication year2014
SampleMurine myotubues
Sample nameC2C12 - MT
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV140002: EV-METRIC:67%
41
Experiment ID678
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 29951053   
OrganismMus musculus
Experiment descriptionImmunoregulatory Effects of Myeloid-Derived Suppressor Cell Exosomes in Mouse Model of Autoimmune Alopecia Areata.
AuthorsZör M, Zhao K, Kutlu N, Bauer N, Provaznik J, Hackert T, Schnör M.
Journal name Front Immunol
Publication year2018
SampleMyeloid-derived suppressor cells
Sample nameIn-vitro- Alopecia areata affected C3H/HeJ mice BMC derived
Isolation/purification methodsUltracentrifugation
Density gradient centrifugation
Flotation densityNot provided
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
42
Experiment ID679
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 29951053   
OrganismMus musculus
Experiment descriptionImmunoregulatory Effects of Myeloid-Derived Suppressor Cell Exosomes in Mouse Model of Autoimmune Alopecia Areata.
AuthorsZör M, Zhao K, Kutlu N, Bauer N, Provaznik J, Hackert T, Schnör M.
Journal name Front Immunol
Publication year2018
SampleMyeloid-derived suppressor cells
Sample nameEx-vivo- Alopecia areata affected C3H/HeJ mice
Isolation/purification methodsUltracentrifugation
Density gradient centrifugation
Flotation densityNot provided
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
43
Experiment ID1126
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 24295599   
OrganismMus musculus
Experiment descriptionExosomes from myeloid-derived suppressor cells carry biologically active proteins.
AuthorsBurke M, Choksawangkarn W, Edwards N, Ostrand-Rosenberg S, Fenselau C.
Journal name J Proteome Res
Publication year2014
SampleMyeloid-derived suppressor cells
Sample nameConventional MDSC's-BALB/c mice injected with 4T1 cells
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140063: EV-METRIC:71%
44
Experiment ID1127
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 24295599   
OrganismMus musculus
Experiment descriptionExosomes from myeloid-derived suppressor cells carry biologically active proteins.
AuthorsBurke M, Choksawangkarn W, Edwards N, Ostrand-Rosenberg S, Fenselau C.
Journal name J Proteome Res
Publication year2014
SampleMyeloid-derived suppressor cells
Sample nameInflammatory MDSC's-BALB/c mice injected with 4T1 cells expressing IL-1.
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140063: EV-METRIC:71%
45
Experiment ID33
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 21136642   
OrganismMus musculus
Experiment descriptionOligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
AuthorsEva-Maria Krär-Albers, Niko Bretz, Stefan Tenzer, Christine Winterstein, Wiebke Mös, Hendrik Berger, Klaus-Armin Nave, HansjöSchild, Jacqueline Trotter
Journal name PROTEOMICS_CL
Publication year2007
SampleOligodendrocytes
Sample nameOligodendrocytes
Oli-neu
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density1.10-1.14 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [QTOF]
Western blotting
Immunoelectron microscopy
EV-TRACK -
46
Experiment ID174
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Western blotting
PubMed ID 21842121   
OrganismMus musculus
Experiment descriptionOligodendroglioma cells shed microvesicles which contain TRAIL as well as molecular chaperones and induce cell death in astrocytes
AuthorsLo Cicero A, Schiera G, Proia P, Saladino P, Savettieri G, Di Liegro CM, Di Liegro I
Journal name Int J Oncol
Publication year2011
SampleOligodendrocytes
Sample nameG26/24
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -
47
Experiment ID419
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 21136642   
OrganismMus musculus
Experiment descriptionOligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
AuthorsKrÃmer-Albers EM, Bretz N, Tenzer S, Winterstein C, Möbius W, Berger H, Nave KA, Schild H, Trotter J.
Journal name Proteomics Clin Appl
Publication year2007
SampleOligodendrocytes
Sample nameNormal-Oligodendrocytes
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
48
Experiment ID372
Identified moleculeprotein
Extracellular vesicle typeMatrix vesicles
Identification method Mass spectrometry
PubMed ID 17096383   
OrganismMus musculus
Experiment descriptionAnalysis of the extracellular matrix vesicle proteome in mineralizing osteoblasts.
AuthorsXiao Z, Camalier CE, Nagashima K, Chan KC, Lucas DA, de la Cruz MJ, Gignac M, Lockett S, Issaq HJ, Veenstra TD, Conrads TP, Beck GR Jr.
Journal name J Cell Physiol
Publication year2007
SampleOsteoblasts
Sample nameMC3T3-E1
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
49
Experiment ID392
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 19351151   
OrganismMus musculus
Experiment descriptionCharacterization of vesicles secreted from insulinoma NIT-1 cells.
AuthorsLee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year2009
SamplePancreatic cells
Sample namePancreatic beta cell (NIT-1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
50
Experiment ID1007
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23463506   
OrganismMus musculus
Experiment descriptionThe intracellular interactome of tetraspanin-enriched microdomains reveals their function as sorting machineries toward exosomes.
AuthorsPerez-Hernandez D, Gutiéez-Váuez C, Jorge I, Ló-MartíS, Ursa A, Sáhez-Madrid F, Váuez J, Yáz-Mó
Journal name J Biol Chem.
Publication year2013
SampleT cells
Sample nameT lymphoblasts - CD81 KO mice
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV130110: EV-METRIC:22%
51
Experiment ID191
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry [QTOF]
PubMed ID 21635979   
OrganismMus musculus
Experiment descriptionProteomic characterization of thymocyte-derived microvesicles and apoptotic bodies in BALB/c mice
AuthorsTuriáL, MisjáP, Szabó, Aradi B, Páczi K, Ozohanics O, Drahos L, Kittel A, Falus A, BuzáEI, Véy K
Journal name J Proteomics
Publication year2011
SampleThymus
Sample nameThymocytes - Normal
Isolation/purification methodsDifferential centrifugation
Filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
FACS
Mass spectrometry [QTOF]
EV-TRACK EV110117: EV-METRIC:11%, 11%
52
Experiment ID192
Identified moleculeprotein
Extracellular vesicle typeApoptotic bodies
Identification method Mass spectrometry [QTOF]
PubMed ID 21635979   
OrganismMus musculus
Experiment descriptionProteomic characterization of thymocyte-derived microvesicles and apoptotic bodies in BALB/c mice
AuthorsTuriáL, MisjáP, Szabó, Aradi B, Páczi K, Ozohanics O, Drahos L, Kittel A, Falus A, BuzáEI, Véy K
Journal name J Proteomics
Publication year2011
SampleThymus
Sample nameThymocytes - Normal
Isolation/purification methodsDifferential centrifugation
Filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
FACS
Mass spectrometry [QTOF]
EV-TRACK EV110117: EV-METRIC:11%, 11%