Gene description for Eef1a1
Gene name	eukaryotic translation elongation factor 1 alpha 1
Gene symbol	Eef1a1
Other names/aliases	EEF-1 EEF1A EF1A Eef1a2 Eef1a2l1 SI
Species	Rattus norvegicus
Database cross references - Eef1a1
Vesiclepedia	VP_171361
ExoCarta	ExoCarta_171361
Entrez Gene	171361
Eef1a1 identified in extracellular vesicles derived from the following tissue/cell type
Hepatocytes [Exosomes]      More >>>	19367702
Insulinoma cells [Exosomes]      More >>>	27872147
Insulinoma cells [Exosomes]      More >>>	27872147
Insulinoma cells [Microparticles]      More >>>	22345510
Insulinoma cells [Microparticles]      More >>>	22345510
Insulinoma cells [Microparticles]      More >>>	22345510
Reticulocytes [Exosomes]      More >>>	21828046
Reticulocytes [Exosomes]      More >>>	21828046
Reticulocytes [Exosomes]      More >>>	21828046
Reticulocytes [Exosomes]      More >>>	21828046
Reticulocytes [Exosomes]      More >>>	21828046
Reticulocytes [Exosomes]      More >>>	21828046
Reticulocytes [Exosomes]      More >>>	21828046
Reticulocytes [Exosomes]      More >>>	21828046
Reticulocytes [Exosomes]      More >>>	21828046
Retinal glial cells [Exosomes]      More >>>	24400796
Experiment description of studies that identified Eef1a1 in extracellular vesicles
1 Experiment ID 35 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry PubMed ID 19367702    Organism Rattus norvegicus Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes. Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM Journal name JPR Publication year 2008 Sample Hepatocytes Sample name Hepatocytes Isolation/purification methods Differential centrifugation Filtration Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [QTOF] Western blotting EV-TRACK -
2 Experiment ID 1220 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry PubMed ID 27872147    Organism Rattus norvegicus Experiment description Primary Human and Rat .-Cells Release the Intracellular Autoantigens GAD65, IA-2, and Proinsulin in Exosomes Together With Cytokine-Induced Enhancers of Immunity. Authors Cianciaruso C, Phelps EA, Pasquier M, Hamelin R, Demurtas D, Alibashe Ahmed M, Piemonti L, Hirosue S, Swartz MA, De Palma M, Hubbell JA, Baekkeskov S. Journal name Diabetes. Publication year 2017 Sample Insulinoma cells Sample name INS-1E cells Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV160008: EV-METRIC:55%, 44%, 33%
3 Experiment ID 1221 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry PubMed ID 27872147    Organism Rattus norvegicus Experiment description Primary Human and Rat .-Cells Release the Intracellular Autoantigens GAD65, IA-2, and Proinsulin in Exosomes Together With Cytokine-Induced Enhancers of Immunity. Authors Cianciaruso C, Phelps EA, Pasquier M, Hamelin R, Demurtas D, Alibashe Ahmed M, Piemonti L, Hirosue S, Swartz MA, De Palma M, Hubbell JA, Baekkeskov S. Journal name Diabetes. Publication year 2017 Sample Insulinoma cells Sample name Rat interleukin (IL)-1. (10 units/mL) Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV160008: EV-METRIC:55%, 44%, 33%
4 Experiment ID 1279 Identified molecule protein Extracellular vesicle type Microparticles Identification method Mass spectrometry PubMed ID 22345510    Organism Rattus norvegicus Experiment description Characterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies. Authors Palmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR. Journal name Mol Cell Proteomics. Publication year 2012 Sample Insulinoma cells Sample name NHI 6F Tu28, cytokine-stimulated Replicate 1 Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV120151: EV-METRIC:14%, 0%
5 Experiment ID 1280 Identified molecule protein Extracellular vesicle type Microparticles Identification method Mass spectrometry PubMed ID 22345510    Organism Rattus norvegicus Experiment description Characterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies. Authors Palmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR. Journal name Mol Cell Proteomics. Publication year 2012 Sample Insulinoma cells Sample name NHI 6F Tu28, cytokine-stimulated Replicate 2 Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV120151: EV-METRIC:14%, 0%
6 Experiment ID 1281 Identified molecule protein Extracellular vesicle type Microparticles Identification method Mass spectrometry PubMed ID 22345510    Organism Rattus norvegicus Experiment description Characterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies. Authors Palmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR. Journal name Mol Cell Proteomics. Publication year 2012 Sample Insulinoma cells Sample name NHI 6F Tu28, cytokine-stimulated Replicate 3 Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV120151: EV-METRIC:14%, 0%
7 Experiment ID 90 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046    Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D2 Rep 1 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV110005: EV-METRIC:44%
8 Experiment ID 94 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046    Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D4 Rep 1 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV110005: EV-METRIC:44%
9 Experiment ID 95 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046    Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D7 Rep 1 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.13-1.25 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV110005: EV-METRIC:44%
10 Experiment ID 96 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046    Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D2 Rep 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV110005: EV-METRIC:44%
11 Experiment ID 97 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046    Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D4 Rep 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV110005: EV-METRIC:44%
12 Experiment ID 98 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046    Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D7 Rep 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.13-1.25 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV110005: EV-METRIC:44%
13 Experiment ID 99 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry [QSTAR] PubMed ID 21828046    Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D2 Rep 3 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV110005: EV-METRIC:44%
14 Experiment ID 100 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry [QSTAR] PubMed ID 21828046    Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D4 Rep 3 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV110005: EV-METRIC:44%
15 Experiment ID 101 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry [QSTAR] PubMed ID 21828046    Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D7 Rep 3 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.13-1.25 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV110005: EV-METRIC:44%
16 Experiment ID 1259 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry PubMed ID 24400796    Organism Rattus norvegicus Experiment description Exosomal proteins in the aqueous humor as novel biomarkers in patients with neovascular age-related macular degeneration. Authors Kang GY, Bang JY, Choi AJ, Yoon J, Lee WC, Choi S, Yoon S, Kim HC, Baek JH, Park HS, Lim HJ, Chung H. Journal name J Proteome Res. Publication year 2014 Sample Retinal glial cells Sample name Mücells Isolation/purification methods ExoQuick Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV140174: EV-METRIC:13%, 13%