Gene description for Eef1a1
Gene name eukaryotic translation elongation factor 1 alpha 1
Gene symbol Eef1a1
Other names/aliases EEF-1
EEF1A
EF1A
Eef1a2
Eef1a2l1
SI
Species Rattus norvegicus
 Database cross references - Eef1a1
Vesiclepedia VP_171361
ExoCarta ExoCarta_171361
Entrez Gene 171361
 Eef1a1 identified in extracellular vesicles derived from the following tissue/cell type
Hepatocytes [Exosomes]      More >>> 19367702   
Insulinoma cells [Exosomes]      More >>> 27872147   
Insulinoma cells [Exosomes]      More >>> 27872147   
Insulinoma cells [Microparticles]      More >>> 22345510   
Insulinoma cells [Microparticles]      More >>> 22345510   
Insulinoma cells [Microparticles]      More >>> 22345510   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Retinal glial cells [Exosomes]      More >>> 24400796   
 Experiment description of studies that identified Eef1a1 in extracellular vesicles
1
Experiment ID35
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 19367702   
OrganismRattus norvegicus
Experiment descriptionCharacterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
AuthorsConde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year2008
SampleHepatocytes
Sample nameHepatocytes
Isolation/purification methodsDifferential centrifugation
Filtration
Sucrose density gradient
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [QTOF]
Western blotting
EV-TRACK -
2
Experiment ID1220
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 27872147   
OrganismRattus norvegicus
Experiment descriptionPrimary Human and Rat .-Cells Release the Intracellular Autoantigens GAD65, IA-2, and Proinsulin in Exosomes Together With Cytokine-Induced Enhancers of Immunity.
AuthorsCianciaruso C, Phelps EA, Pasquier M, Hamelin R, Demurtas D, Alibashe Ahmed M, Piemonti L, Hirosue S, Swartz MA, De Palma M, Hubbell JA, Baekkeskov S.
Journal name Diabetes.
Publication year2017
SampleInsulinoma cells
Sample nameINS-1E cells
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV160008: EV-METRIC:55%, 44%, 33%
3
Experiment ID1221
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 27872147   
OrganismRattus norvegicus
Experiment descriptionPrimary Human and Rat .-Cells Release the Intracellular Autoantigens GAD65, IA-2, and Proinsulin in Exosomes Together With Cytokine-Induced Enhancers of Immunity.
AuthorsCianciaruso C, Phelps EA, Pasquier M, Hamelin R, Demurtas D, Alibashe Ahmed M, Piemonti L, Hirosue S, Swartz MA, De Palma M, Hubbell JA, Baekkeskov S.
Journal name Diabetes.
Publication year2017
SampleInsulinoma cells
Sample nameRat interleukin (IL)-1. (10 units/mL)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV160008: EV-METRIC:55%, 44%, 33%
4
Experiment ID1279
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 1
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%
5
Experiment ID1280
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 2
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%
6
Experiment ID1281
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 3
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%
7
Experiment ID90
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D2 Rep 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.16-1.21 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
8
Experiment ID94
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D4 Rep 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.16-1.21 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
9
Experiment ID95
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D7 Rep 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.13-1.25 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
10
Experiment ID96
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D2 Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.16-1.21 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
11
Experiment ID97
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D4 Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.16-1.21 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
12
Experiment ID98
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D7 Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.13-1.25 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
13
Experiment ID99
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D2 Rep 3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.16-1.21 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
14
Experiment ID100
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D4 Rep 3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.16-1.21 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
15
Experiment ID101
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D7 Rep 3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.13-1.25 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
16
Experiment ID1259
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 24400796   
OrganismRattus norvegicus
Experiment descriptionExosomal proteins in the aqueous humor as novel biomarkers in patients with neovascular age-related macular degeneration.
AuthorsKang GY, Bang JY, Choi AJ, Yoon J, Lee WC, Choi S, Yoon S, Kim HC, Baek JH, Park HS, Lim HJ, Chung H.
Journal name J Proteome Res.
Publication year2014
SampleRetinal glial cells
Sample nameMücells
Isolation/purification methodsExoQuick
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140174: EV-METRIC:13%, 13%