Gene description for Rab5c
Gene name RAB5C, member RAS oncogene family
Gene symbol Rab5c
Other names/aliases AI326010
Rabl
Species Mus musculus
 Database cross references - Rab5c
Vesiclepedia VP_19345
ExoCarta ExoCarta_19345
Entrez Gene 19345
 Rab5c identified in extracellular vesicles derived from the following tissue/cell type
adipocytes [Exosomes]      More >>> 24513287   
adipocytes [Exosomes]      More >>> 24513287   
Basophilic leukemia cells [Exosomes]      More >>> 20424270   
Breast cancer cells [Exosomes]      More >>> 29115712   
Breast cancer cells [Exosomes]      More >>> 29115712   
Breast cancer cells [Exosomes]      More >>> 29115712   
Breast cancer cells [Exosomes]      More >>> 29115712   
Embryonic fibroblasts [Exosomes]      More >>> 18494037   
Mast cells [Exosomes]      More >>> 17486113   
Melanoma cells [Extracellular vesicles]      More >>> 29907695   
Melanoma cells [Extracellular vesicles]      More >>> 29907695   
Melanoma cells [Extracellular vesicles]      More >>> 29907695   
Melanoma cells [Extracellular vesicles]      More >>> 29907695   
Mouse embryonic fibroblasts [Extracellular vesicles]      More >>> 30035390   
Mouse embryonic fibroblasts [Extracellular vesicles]      More >>> 30035390   
Mouse embryonic fibroblasts [Extracellular vesicles]      More >>> 30035390   
Mouse embryonic fibroblasts [Extracellular vesicles]      More >>> 30035390   
Mov neuroglial cell [Exosomes]      More >>> 15210972   
Murine myoblast [Extracellular vesicles]      More >>> 24392111   
Murine myotubues [Extracellular vesicles]      More >>> 24392111   
T cells [Exosomes]      More >>> 23463506   
Thymus [Microvesicles]      More >>> 21635979   
Thymus [Apoptotic bodies]      More >>> 21635979   
 Experiment description of studies that identified Rab5c in extracellular vesicles
1
Experiment ID1097
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 24513287   
OrganismMus musculus
Experiment descriptionLipid synthesis is promoted by hypoxic adipocyte-derived exosomes in 3T3-L1 cells.
AuthorsSano S, Izumi Y, Yamaguchi T, Yamazaki T, Tanaka M, Shiota M, Osada-Oka M, Nakamura Y, Wei M, Wanibuchi H, Iwao H, Yoshiyama M.
Journal name Biochem Biophys Res Commun
Publication year2014
Sampleadipocytes
Sample name3T3-L1- Normoxic conditions
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140281: EV-METRIC:0%, 22%
2
Experiment ID1098
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 24513287   
OrganismMus musculus
Experiment descriptionLipid synthesis is promoted by hypoxic adipocyte-derived exosomes in 3T3-L1 cells.
AuthorsSano S, Izumi Y, Yamaguchi T, Yamazaki T, Tanaka M, Shiota M, Osada-Oka M, Nakamura Y, Wei M, Wanibuchi H, Iwao H, Yoshiyama M.
Journal name Biochem Biophys Res Commun
Publication year2014
Sampleadipocytes
Sample name3T3-L1- Hypoxic conditions
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140281: EV-METRIC:0%, 22%
3
Experiment ID89
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 20424270   
OrganismRattus norvegicus
Experiment descriptionExosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins.
AuthorsSubra C, Grand D, Laulagnier K, Stella A, Lambeau G, Paillasse M, De Medina P, Monsarrat B, Perret B, Silvente-Poirot S, Poirot M, Record M.
Journal name JLR
Publication year2010
SampleBasophilic leukemia cells
Sample nameRBL-2H3
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density-
Molecules identified in the studyProtein
Lipids
Methods used in the studyWestern blotting
Gas chromatography
Thin layer chromatography
Mass spectrometry [Qstar XL]
FACS
EV-TRACK EV100089: EV-METRIC:0%
4
Experiment ID1051
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 29115712   
OrganismMus musculus
Experiment descriptionProteomic Profiling of Exosomes Secreted by Breast Cancer Cells with Varying Metastatic Potential.
AuthorsGangoda L, Liem M, Ang CS, Keerthikumar S, Adda CG, Parker BS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleBreast cancer cells
Sample name67NR
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -
5
Experiment ID1052
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 29115712   
OrganismMus musculus
Experiment descriptionProteomic Profiling of Exosomes Secreted by Breast Cancer Cells with Varying Metastatic Potential.
AuthorsGangoda L, Liem M, Ang CS, Keerthikumar S, Adda CG, Parker BS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleBreast cancer cells
Sample name66cl4
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -
6
Experiment ID1053
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 29115712   
OrganismMus musculus
Experiment descriptionProteomic Profiling of Exosomes Secreted by Breast Cancer Cells with Varying Metastatic Potential.
AuthorsGangoda L, Liem M, Ang CS, Keerthikumar S, Adda CG, Parker BS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleBreast cancer cells
Sample name4T1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -
7
Experiment ID1054
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 29115712   
OrganismMus musculus
Experiment descriptionProteomic Profiling of Exosomes Secreted by Breast Cancer Cells with Varying Metastatic Potential.
AuthorsGangoda L, Liem M, Ang CS, Keerthikumar S, Adda CG, Parker BS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleBreast cancer cells
Sample name4T1.2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -
8
Experiment ID73
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 18494037   
OrganismMus musculus
Experiment descriptionDifference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
AuthorsJi H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year2008
SampleEmbryonic fibroblasts
Sample nameNIH3T3
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LCQ DECA]
Western blotting
EV-TRACK -
9
Experiment ID15
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 17486113   
OrganismMus musculus
Homo sapiens
Experiment descriptionExosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
AuthorsValadi H, Ekströ, Bossios A, Sjöand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year2007
SampleMast cells
Sample nameMC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methodsFiltration
Ultracentrifugation
Sucrose density gradient
Flotation density1.11-1.21 g/mL
Molecules identified in the studyProtein
mRNA
miRNA
Methods used in the studyMass spectrometry [MALDI TOF]
Western blotting
Microarray
MicroRNA array [miRCURY LNA Array]
EV-TRACK -
10
Experiment ID1167
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 29907695   
OrganismMus musculus
Experiment descriptionCancer cells copy migratory behavior and exchange signaling networks via extracellular vesicles.
AuthorsSteenbeek SC, Pham TV, de Ligt J, Zomer A, Knol JC, Piersma SR, Schelfhorst T, Huisjes R, Schiffelers RM, Cuppen E, Jimenez CR, van Rheenen J.
Journal name EMBO J.
Publication year2018
SampleMelanoma cells
Sample nameB16F1 (16.5 K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyMass spectrometry
RNA sequencing
EV-TRACK -
11
Experiment ID1168
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 29907695   
OrganismMus musculus
Experiment descriptionCancer cells copy migratory behavior and exchange signaling networks via extracellular vesicles.
AuthorsSteenbeek SC, Pham TV, de Ligt J, Zomer A, Knol JC, Piersma SR, Schelfhorst T, Huisjes R, Schiffelers RM, Cuppen E, Jimenez CR, van Rheenen J.
Journal name EMBO J.
Publication year2018
SampleMelanoma cells
Sample name B16F10 (16.5 K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyMass spectrometry
RNA sequencing
EV-TRACK -
12
Experiment ID1169
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 29907695   
OrganismMus musculus
Experiment descriptionCancer cells copy migratory behavior and exchange signaling networks via extracellular vesicles.
AuthorsSteenbeek SC, Pham TV, de Ligt J, Zomer A, Knol JC, Piersma SR, Schelfhorst T, Huisjes R, Schiffelers RM, Cuppen E, Jimenez CR, van Rheenen J.
Journal name EMBO J.
Publication year2018
SampleMelanoma cells
Sample nameB16F1 (100 K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyMass spectrometry
RNA sequencing
EV-TRACK -
13
Experiment ID1170
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 29907695   
OrganismMus musculus
Experiment descriptionCancer cells copy migratory behavior and exchange signaling networks via extracellular vesicles.
AuthorsSteenbeek SC, Pham TV, de Ligt J, Zomer A, Knol JC, Piersma SR, Schelfhorst T, Huisjes R, Schiffelers RM, Cuppen E, Jimenez CR, van Rheenen J.
Journal name EMBO J.
Publication year2018
SampleMelanoma cells
Sample name B16F10 (100 K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyMass spectrometry
RNA sequencing
EV-TRACK -
14
Experiment ID1055
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30035390   
OrganismMus musculus
Experiment descriptionArrestin-domain Containing Protein 1 (Arrdc1) Regulates the Protein Cargo and Release of Extracellular Vesicles.
AuthorsAnand S, Foot N, Ang CS, Gembus KM, Keerthikumar S, Adda CG, Mathivanan S, Kumar S.
Journal name Proteomics.
Publication year2018
SampleMouse embryonic fibroblasts
Sample nameMEFs - WT 10K
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -
15
Experiment ID1056
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30035390   
OrganismMus musculus
Experiment descriptionArrestin-domain Containing Protein 1 (Arrdc1) Regulates the Protein Cargo and Release of Extracellular Vesicles.
AuthorsAnand S, Foot N, Ang CS, Gembus KM, Keerthikumar S, Adda CG, Mathivanan S, Kumar S.
Journal name Proteomics.
Publication year2018
SampleMouse embryonic fibroblasts
Sample nameMEFs - WT exosomes
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -
16
Experiment ID1057
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30035390   
OrganismMus musculus
Experiment descriptionArrestin-domain Containing Protein 1 (Arrdc1) Regulates the Protein Cargo and Release of Extracellular Vesicles.
AuthorsAnand S, Foot N, Ang CS, Gembus KM, Keerthikumar S, Adda CG, Mathivanan S, Kumar S.
Journal name Proteomics.
Publication year2018
SampleMouse embryonic fibroblasts
Sample nameMEFs - Arrdc1 KO 10K
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -
17
Experiment ID1058
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30035390   
OrganismMus musculus
Experiment descriptionArrestin-domain Containing Protein 1 (Arrdc1) Regulates the Protein Cargo and Release of Extracellular Vesicles.
AuthorsAnand S, Foot N, Ang CS, Gembus KM, Keerthikumar S, Adda CG, Mathivanan S, Kumar S.
Journal name Proteomics.
Publication year2018
SampleMouse embryonic fibroblasts
Sample nameMEFs - Arrdc1 KO exosomes
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -
18
Experiment ID39
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 15210972   
OrganismMus musculus
Experiment descriptionCells release prions in association with exosomes.
AuthorsFevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name PNAS
Publication year2004
SampleMov neuroglial cell
Sample nameMov neuroglial cell
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density1.14 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [QSTAR]
Western blotting
Immunoelectron microscopy
EV-TRACK -
19
Experiment ID996
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 24392111   
OrganismMus musculus
Experiment descriptionProteomic analysis of C2C12 myoblast and myotube exosome-like vesicles: a new paradigm for myoblast-myotube cross talk?
AuthorsForterre A, Jalabert A, Berger E, Baudet M, Chikh K, Errazuriz E, De Larichaudy J, Chanon S, Weiss-Gayet M, Hesse AM, Record M, Geloen A, Lefai E, Vidal H, Couté, Rome S.
Journal name PLoS One.
Publication year2014
SampleMurine myoblast
Sample nameC2C12 - MB
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV140002: EV-METRIC:67%
20
Experiment ID997
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 24392111   
OrganismMus musculus
Experiment descriptionProteomic analysis of C2C12 myoblast and myotube exosome-like vesicles: a new paradigm for myoblast-myotube cross talk?
AuthorsForterre A, Jalabert A, Berger E, Baudet M, Chikh K, Errazuriz E, De Larichaudy J, Chanon S, Weiss-Gayet M, Hesse AM, Record M, Geloen A, Lefai E, Vidal H, Couté, Rome S.
Journal name PLoS One.
Publication year2014
SampleMurine myotubues
Sample nameC2C12 - MT
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV140002: EV-METRIC:67%
21
Experiment ID1007
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23463506   
OrganismMus musculus
Experiment descriptionThe intracellular interactome of tetraspanin-enriched microdomains reveals their function as sorting machineries toward exosomes.
AuthorsPerez-Hernandez D, Gutiéez-Váuez C, Jorge I, Ló-MartíS, Ursa A, Sáhez-Madrid F, Váuez J, Yáz-Mó
Journal name J Biol Chem.
Publication year2013
SampleT cells
Sample nameT lymphoblasts - CD81 KO mice
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV130110: EV-METRIC:22%
22
Experiment ID191
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry [QTOF]
PubMed ID 21635979   
OrganismMus musculus
Experiment descriptionProteomic characterization of thymocyte-derived microvesicles and apoptotic bodies in BALB/c mice
AuthorsTuriáL, MisjáP, Szabó, Aradi B, Páczi K, Ozohanics O, Drahos L, Kittel A, Falus A, BuzáEI, Véy K
Journal name J Proteomics
Publication year2011
SampleThymus
Sample nameThymocytes - Normal
Isolation/purification methodsDifferential centrifugation
Filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
FACS
Mass spectrometry [QTOF]
EV-TRACK EV110117: EV-METRIC:11%, 11%
23
Experiment ID192
Identified moleculeprotein
Extracellular vesicle typeApoptotic bodies
Identification method Mass spectrometry [QTOF]
PubMed ID 21635979   
OrganismMus musculus
Experiment descriptionProteomic characterization of thymocyte-derived microvesicles and apoptotic bodies in BALB/c mice
AuthorsTuriáL, MisjáP, Szabó, Aradi B, Páczi K, Ozohanics O, Drahos L, Kittel A, Falus A, BuzáEI, Véy K
Journal name J Proteomics
Publication year2011
SampleThymus
Sample nameThymocytes - Normal
Isolation/purification methodsDifferential centrifugation
Filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
FACS
Mass spectrometry [QTOF]
EV-TRACK EV110117: EV-METRIC:11%, 11%