Gene description for ALCAM
Gene name activated leukocyte cell adhesion molecule
Gene symbol ALCAM
Other names/aliases CD166
MEMD
Species Homo sapiens
 Database cross references - ALCAM
Vesiclepedia VP_214
ExoCarta ExoCarta_214
Entrez Gene 214
HGNC 400
MIM 601662
 ALCAM identified in extracellular vesicles derived from the following tissue/cell type
Astrocytoma cells [Extracellular vesicles]      More >>> 30006486   
Astrocytoma cells [Extracellular vesicles]      More >>> 30006486   
Astrocytoma cells [Extracellular vesicles]      More >>> 30006486   
Astrocytoma cells [Extracellular vesicles]      More >>> 30006486   
Astrocytoma cells [Extracellular vesicles]      More >>> 30006486   
Astrocytoma cells [Extracellular vesicles]      More >>> 30006486   
B cells [Exosomes]      More >>> 20458337   
B cells [Exosomes]      More >>> 20458337   
B cells [Exosomes]      More >>> 20458337   
Brain cancer cells [Extracellular vesicles]      More >>> 27894104   
Breast cancer cells [Extracellular vesicles]      More >>> 27894104   
Breast cancer cells [Extracellular vesicles]      More >>> 27894104   
Breast cancer cells [Extracellular vesicles]      More >>> 26378940   
Bronchial epithelial cells [Extracellular vesicles]      More >>> 29127410   
Bronchial epithelial cells [Extracellular vesicles]      More >>> 29127410   
Bronchial epithelial cells [Extracellular vesicles]      More >>> 29127410   
Bronchial epithelial cells [Extracellular vesicles]      More >>> 29127410   
Bronchial epithelial cells [Extracellular vesicles]      More >>> 29127410   
Chronic lymphocytic leukemia cells [Exosomes]      More >>> 26100252   
Colorectal cancer cells [Microvesicles]      More >>> 19930720   
Colorectal cancer cells [Exosomes]      More >>> 23161513   
Colorectal cancer cells [Exosomes]      More >>> 23161513   
Colorectal cancer cells [Exosomes]      More >>> 23161513   
Colorectal cancer cells [Extracellular vesicles]      More >>> 27894104   
Colorectal cancer cells [Extracellular vesicles]      More >>> 27894104   
Colorectal cancer cells [Extracellular vesicles]      More >>> 27894104   
Colorectal cancer cells [Microvesicles]      More >>> 28842968   
Colorectal cancer cells [Microvesicles]      More >>> 28842968   
Colorectal cancer cells [Extracellular vesicles]      More >>> 28842968   
Colorectal cancer cells [Extracellular vesicles]      More >>> 28842968   
Dendritic cells [Microvesicles]      More >>> 26858453   
Dendritic cells [Microvesicles]      More >>> 26858453   
Dendritic cells [Exosomes]      More >>> 26858453   
Dendritic cells [Exosomes]      More >>> 26858453   
Endothelial cells [Exosomes/Extracellular vesicles]      More >>> 24009886   
Epithelial cells [Exosomes]      More >>> 25776846   
Glioblastoma cells [Exosomes]      More >>> 25802036   
Glioblastoma cells [Exosomes]      More >>> 29937354   
Kidney cancer cells [Extracellular vesicles]      More >>> 27894104   
Leukemia cells [Extracellular vesicles]      More >>> 27894104   
Leukemia cells [Extracellular vesicles]      More >>> 27894104   
Leukemia cells [Extracellular vesicles]      More >>> 27894104   
Lung cancer cells [Extracellular vesicles]      More >>> 27894104   
Lung cancer cells [Extracellular vesicles]      More >>> 27894104   
Lung cancer cells [Extracellular vesicles]      More >>> 27894104   
Mesenchymal stem cells [Exosomes]      More >>> Unpublished / Not applicable
Mesenchymal stem cells [Microvesicles]      More >>> 22148876   
Mesenchymal stem cells [Microvesicles]      More >>> 22148876   
Monocytes [Microparticles]      More >>> 19548909   
Monocytes [Microparticles]      More >>> 19548909   
Monocytes [Microparticles]      More >>> 19548909   
Monocytes [Microparticles]      More >>> 19548909   
Neonatal myoblast cells [Microvesicles/Nanovesicles]      More >>> 23000592   
Neonatal myoblast cells [Microvesicles/Nanovesicles]      More >>> 23000592   
Ovarian cancer cells [Exosomes]      More >>> 23333927   
Ovarian cancer cells [Exosomes]      More >>> 23333927   
Ovarian cancer cells [Extracellular vesicles]      More >>> 27894104   
Ovarian cancer cells [Extracellular vesicles]      More >>> 27894104   
Ovarian cancer cells [Extracellular vesicles]      More >>> 27894104   
Ovarian cancer cells [Exosomes]      More >>> 24434149   
Ovarian cancer cells [Exosomes]      More >>> 24434149   
Ovarian cancer cells [Exosomes]      More >>> 24434149   
Ovarian cancer cells [Exosomes]      More >>> 24434149   
Prostate cancer cells [Extracellular vesicles]      More >>> 27894104   
Prostate cancer cells [Exosomes]      More >>> 25844599   
Prostate cancer cells [Exosomes]      More >>> 25844599   
Thymus [Exosomes]      More >>> 23844026   
Umblical cord mesenchymal stem cells [Microvesicles]      More >>> 29148239   
Umblical cord mesenchymal stem cells [Microvesicles]      More >>> 29148239   
Umblical cord mesenchymal stem cells [Microvesicles]      More >>> 29148239   
Umblical cord mesenchymal stem cells [Microvesicles]      More >>> 29148239   
Umblical cord mesenchymal stem cells [Microvesicles]      More >>> 29148239   
Umblical cord mesenchymal stem cells [Microvesicles]      More >>> 29148239   
Urine [Exosomes]      More >>> 19056867   
Urine [Exosomes/Membrane vesicles]      More >>> 22106071   
 Experiment description of studies that identified ALCAM in extracellular vesicles
1
Experiment ID1059
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30006486   
OrganismHomo sapiens
Experiment descriptionThe impact of oncogenic EGFRvIII on the proteome of extracellular vesicles released from glioblastoma cells.
AuthorsChoi D, Montermini L, Kim DK, Meehan B, Roth FP, Rak J.
Journal name Mol Cell Proteomics.
Publication year2018
SampleAstrocytoma cells
Sample nameU373 - EVs 1
Isolation/purification methodsFiltration
Ultracentrifugation
Density gradient centrifugation
Flotation density1.09-1.13 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -
2
Experiment ID1060
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30006486   
OrganismHomo sapiens
Experiment descriptionThe impact of oncogenic EGFRvIII on the proteome of extracellular vesicles released from glioblastoma cells.
AuthorsChoi D, Montermini L, Kim DK, Meehan B, Roth FP, Rak J.
Journal name Mol Cell Proteomics.
Publication year2018
SampleAstrocytoma cells
Sample nameU373 - EVs 2
Isolation/purification methodsFiltration
Ultracentrifugation
Density gradient centrifugation
Flotation density1.09-1.13 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
3
Experiment ID1061
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30006486   
OrganismHomo sapiens
Experiment descriptionThe impact of oncogenic EGFRvIII on the proteome of extracellular vesicles released from glioblastoma cells.
AuthorsChoi D, Montermini L, Kim DK, Meehan B, Roth FP, Rak J.
Journal name Mol Cell Proteomics.
Publication year2018
SampleAstrocytoma cells
Sample nameU373 - EVs 3
Isolation/purification methodsFiltration
Ultracentrifugation
Density gradient centrifugation
Flotation density1.09-1.13 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
4
Experiment ID1062
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30006486   
OrganismHomo sapiens
Experiment descriptionThe impact of oncogenic EGFRvIII on the proteome of extracellular vesicles released from glioblastoma cells.
AuthorsChoi D, Montermini L, Kim DK, Meehan B, Roth FP, Rak J.
Journal name Mol Cell Proteomics.
Publication year2018
SampleAstrocytoma cells
Sample nameU373vIII - EVs 1
Isolation/purification methodsFiltration
Ultracentrifugation
Density gradient centrifugation
Flotation density1.09-1.13 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -
5
Experiment ID1063
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30006486   
OrganismHomo sapiens
Experiment descriptionThe impact of oncogenic EGFRvIII on the proteome of extracellular vesicles released from glioblastoma cells.
AuthorsChoi D, Montermini L, Kim DK, Meehan B, Roth FP, Rak J.
Journal name Mol Cell Proteomics.
Publication year2018
SampleAstrocytoma cells
Sample nameU373vIII - EVs 2
Isolation/purification methodsFiltration
Ultracentrifugation
Density gradient centrifugation
Flotation density1.09-1.13 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
6
Experiment ID1064
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30006486   
OrganismHomo sapiens
Experiment descriptionThe impact of oncogenic EGFRvIII on the proteome of extracellular vesicles released from glioblastoma cells.
AuthorsChoi D, Montermini L, Kim DK, Meehan B, Roth FP, Rak J.
Journal name Mol Cell Proteomics.
Publication year2018
SampleAstrocytoma cells
Sample nameU373vIII - EVs 3
Isolation/purification methodsFiltration
Ultracentrifugation
Density gradient centrifugation
Flotation density1.09-1.13 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
7
Experiment ID79
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 20458337   
OrganismHomo sapiens
Experiment descriptionMHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
AuthorsBuschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year2010
SampleB cells
Sample nameRN (HLA-DR15)
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [FT-ICR]
Western blotting
EV-TRACK EV100035: EV-METRIC:44%
8
Experiment ID80
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 20458337   
OrganismHomo sapiens
Experiment descriptionMHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
AuthorsBuschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year2010
SampleB cells
Sample nameRN (HLA-DR15)
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [FT-ICR]
Western blotting
EV-TRACK EV100035: EV-METRIC:44%
9
Experiment ID81
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 20458337   
OrganismHomo sapiens
Experiment descriptionMHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
AuthorsBuschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year2010
SampleB cells
Sample nameRN (HLA-DR15)
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [FT-ICR]
Western blotting
EV-TRACK EV100035: EV-METRIC:44%
10
Experiment ID586
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBrain cancer cells
Sample nameU251
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
11
Experiment ID575
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBreast cancer cells
Sample nameBT549
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
12
Experiment ID577
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBreast cancer cells
Sample nameMCF7
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
13
Experiment ID1029
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 26378940   
OrganismHomo sapiens
Experiment descriptionRedefining the Breast Cancer Exosome Proteome by Tandem Mass Tag Quantitative Proteomics and Multivariate Cluster Analysis.
AuthorsClark DJ, Fondrie WE, Liao Z, Hanson PI, Fulton A, Mao L, Yang AJ.
Journal name Anal Chem.
Publication year2015
SampleBreast cancer cells
Sample nameSKBR3B - 100 K pellet
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV150004: EV-METRIC:67%, 38%
14
Experiment ID1207
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 29127410   
OrganismHomo sapiens
Experiment descriptionUltrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies.
AuthorsBenedikter BJ, Bouwman FG, Vajen T, Heinzmann ACA, Grauls G, Mariman EC, Wouters EFM, Savelkoul PH, Lopez-Iglesias C, Koenen RR, Rohde GGU, Stassen FRM.
Journal name Sci Rep.
Publication year2017
SampleBronchial epithelial cells
Sample nameUltrafiltration and size exclusion chromatography sample 1
Isolation/purification methodsUltrafiltration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170061: EV-METRIC:62%, 50%, 50%
15
Experiment ID1208
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 29127410   
OrganismHomo sapiens
Experiment descriptionUltrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies.
AuthorsBenedikter BJ, Bouwman FG, Vajen T, Heinzmann ACA, Grauls G, Mariman EC, Wouters EFM, Savelkoul PH, Lopez-Iglesias C, Koenen RR, Rohde GGU, Stassen FRM.
Journal name Sci Rep.
Publication year2017
SampleBronchial epithelial cells
Sample nameUltrafiltration and size exclusion chromatography sample 2
Isolation/purification methodsUltrafiltration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170061: EV-METRIC:62%, 50%, 50%
16
Experiment ID1209
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 29127410   
OrganismHomo sapiens
Experiment descriptionUltrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies.
AuthorsBenedikter BJ, Bouwman FG, Vajen T, Heinzmann ACA, Grauls G, Mariman EC, Wouters EFM, Savelkoul PH, Lopez-Iglesias C, Koenen RR, Rohde GGU, Stassen FRM.
Journal name Sci Rep.
Publication year2017
SampleBronchial epithelial cells
Sample nameUltrafiltration and size exclusion chromatography sample 3
Isolation/purification methodsUltrafiltration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170061: EV-METRIC:62%, 50%, 50%
17
Experiment ID1210
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 29127410   
OrganismHomo sapiens
Experiment descriptionUltrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies.
AuthorsBenedikter BJ, Bouwman FG, Vajen T, Heinzmann ACA, Grauls G, Mariman EC, Wouters EFM, Savelkoul PH, Lopez-Iglesias C, Koenen RR, Rohde GGU, Stassen FRM.
Journal name Sci Rep.
Publication year2017
SampleBronchial epithelial cells
Sample nameUltrafiltration and size exclusion chromatography sample 4
Isolation/purification methodsUltrafiltration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170061: EV-METRIC:62%, 50%, 50%
18
Experiment ID1211
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 29127410   
OrganismHomo sapiens
Experiment descriptionUltrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies.
AuthorsBenedikter BJ, Bouwman FG, Vajen T, Heinzmann ACA, Grauls G, Mariman EC, Wouters EFM, Savelkoul PH, Lopez-Iglesias C, Koenen RR, Rohde GGU, Stassen FRM.
Journal name Sci Rep.
Publication year2017
SampleBronchial epithelial cells
Sample nameUltrafiltration and size exclusion chromatography sample 5
Isolation/purification methodsUltrafiltration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170061: EV-METRIC:62%, 50%, 50%
19
Experiment ID902
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 26100252   
OrganismHomo sapiens
Experiment descriptionExosomes released by chronic lymphocytic leukemia cells induce the transition of stromal cells into cancer-associated fibroblasts
AuthorsPaggetti J, Haderk F, Seiffert M, Janji B, Distler U, Ammerlaan W, Kim YJ, Adam J, Lichter P, Solary E, Berchem G, Moussay E.
Journal name Blood
Publication year2015
SampleChronic lymphocytic leukemia cells
Sample nameMEC-1
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Density gradient centrifugation
Flotation density1.15-1.17 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
FACS
Western blotting
EV-TRACK EV150015: EV-METRIC:22%, 44%
20
Experiment ID303
Identified moleculemrna
Extracellular vesicle typeMicrovesicles
Identification method Microarray [Illumina]
PubMed ID 19930720   
OrganismHomo sapiens
Experiment descriptionColorectal cancer cell-derived microvesicles are enriched in cell cycle-related mRNAs that promote proliferation of endothelial cells
AuthorsHong BS, Cho JH, Kim H, Choi EJ, Rho S, Kim J, Kim JH, Choi DS, Kim YK, Hwang D, Gho YS
Journal name BMC Genomics
Publication year2009
SampleColorectal cancer cells
Sample nameSW480
Isolation/purification methodsDifferential centrifugation
Ultrafiltration
OptiPrep density gradient
Flotation density1.09 g/mL
Molecules identified in the studyProtein
mRNA
Methods used in the studyWestern blotting
RT-PCR
Microarray [Illumina]
EV-TRACK -
21
Experiment ID480
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23161513   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
AuthorsDemory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year2012
SampleColorectal cancer cells
Sample nameDKO-1
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130004: EV-METRIC:44%
22
Experiment ID481
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23161513   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
AuthorsDemory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year2012
SampleColorectal cancer cells
Sample nameDks-8
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130004: EV-METRIC:44%
23
Experiment ID482
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23161513   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
AuthorsDemory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year2012
SampleColorectal cancer cells
Sample nameDLD-1
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130004: EV-METRIC:44%
24
Experiment ID587
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleColorectal cancer cells
Sample nameColo205
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
25
Experiment ID589
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleColorectal cancer cells
Sample nameHCT-116
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
26
Experiment ID591
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleColorectal cancer cells
Sample nameHT29
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
27
Experiment ID696
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry
PubMed ID 28842968   
OrganismHomo sapiens
Experiment descriptionInsulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles.
AuthorsLiem M, Ang CS, Mathivanan S.
Journal name Proteomics
Publication year2017
SampleColorectal cancer cells
Sample nameLIM1215- insulin induced
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170021: EV-METRIC:44%, 44%
28
Experiment ID697
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry
PubMed ID 28842968   
OrganismHomo sapiens
Experiment descriptionInsulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles.
AuthorsLiem M, Ang CS, Mathivanan S.
Journal name Proteomics
Publication year2017
SampleColorectal cancer cells
Sample nameLIM1215- non-induced
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170021: EV-METRIC:44%, 44%
29
Experiment ID1049
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 28842968   
OrganismHomo sapiens
Experiment descriptionInsulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles.
AuthorsLiem M, Ang CS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleColorectal cancer cells
Sample name LIM1215 - NI
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV170021: EV-METRIC:44%, 44%
30
Experiment ID1050
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 28842968   
OrganismHomo sapiens
Experiment descriptionInsulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles.
AuthorsLiem M, Ang CS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleColorectal cancer cells
Sample namelim1215 - II
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV170021: EV-METRIC:44%, 44%
31
Experiment ID562
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 26858453   
OrganismHomo sapiens
Experiment descriptionProteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
AuthorsKowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Thé C
Journal name PNAS
Publication year2016
SampleDendritic cells
Sample namemonocyte-derived dendritic cells (F3 10K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density1.11 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
32
Experiment ID563
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 26858453   
OrganismHomo sapiens
Experiment descriptionProteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
AuthorsKowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Thé C
Journal name PNAS
Publication year2016
SampleDendritic cells
Sample namemonocyte-derived dendritic cells (F5 10K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density1.14 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
33
Experiment ID564
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [LTQ]
PubMed ID 26858453   
OrganismHomo sapiens
Experiment descriptionProteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
AuthorsKowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Thé C
Journal name PNAS
Publication year2016
SampleDendritic cells
Sample namemonocyte-derived dendritic cells (F3 100K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density1.11 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
34
Experiment ID565
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [LTQ ORBITRAP]
PubMed ID 26858453   
OrganismHomo sapiens
Experiment descriptionProteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
AuthorsKowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Thé C
Journal name PNAS
Publication year2016
SampleDendritic cells
Sample namemonocyte-derived dendritic cells (F5 100K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density1.14 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ ORBITRAP]
EV-TRACK -
35
Experiment ID551
Identified moleculeprotein
Extracellular vesicle typeExosomes/Extracellular vesicles
Identification method Mass spectrometry
PubMed ID 24009886   
OrganismHomo sapiens
Experiment descriptionCellular stress conditions are reflected in the protein and RNA content of endothelial cell-derived exosomes.
Authorsde Jong OG, Verhaar MC, Chen Y, Vader P, Gremmels H, Posthuma G, Schiffelers RM, Gucek M, van Balkom BW.
Journal name J Extracell Vesicles
Publication year2012
SampleEndothelial cells
Sample nameStressed (hypoxia, TNF-alpha-induced activation, high glucose and mannose concentrations)-Endothelial cells (HMEC-1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120004: EV-METRIC:67%
36
Experiment ID862
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 25776846   
OrganismHomo sapiens
Experiment descriptionHuman thymic epithelial primary cells produce exosomes carrying tissue-restricted antigens
AuthorsSkogberg G, Lundberg V, Berglund M, Gudmundsdottir J, Telemo E, Lindgren S, Ekwall O.
Journal name Immunol Cell Biol
Publication year2015
SampleEpithelial cells
Sample nameThymic tissue - culture 2
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV150045: EV-METRIC:25%
37
Experiment ID722
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 25802036   
OrganismHomo sapiens
Experiment descriptionGlioblastoma-derived extracellular vesicles modify the phenotype of monocytic cells.
Authorsde Vrij J, Maas SL, Kwappenberg KM, Schnoor R, Kleijn A, Dekker L, Luider TM, de Witte LD, Litjens M, van Strien ME, Hol EM, Kroonen J, Robe PA, Lamfers ML, Schilham MW, Broekman ML.
Journal name Int J Cancer
Publication year2015
SampleGlioblastoma cells
Sample name U87.MG/EGFRvIII
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV150064: EV-METRIC:0%, 0%, 29%
38
Experiment ID1072
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 29937354   
OrganismHomo sapiens
Experiment descriptionApoptotic Cell-Derived Extracellular Vesicles Promote Malignancy of Glioblastoma Via Intercellular Transfer of Splicing Factors.
AuthorsPavlyukov MS, Yu H, Bastola S, Minata M, Shender VO, Lee Y, Zhang S, Wang J, Komarova S, Wang J, Yamaguchi S, Alsheikh HA, Shi J, Chen D, Mohyeldin A, Kim SH, Shin YJ, Anufrieva K, Evtushenko EG, Antipova NV, Arapidi GP, Govorun V, Pestov NB, Shakhparonov MI, Lee LJ, Nam DH, Nakano I.
Journal name Cancer Cell
Publication year2018
SampleGlioblastoma cells
Sample nameLethally irradiated GBM157 - apoExo - biorep2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
39
Experiment ID599
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleKidney cancer cells
Sample nameSN12C
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
40
Experiment ID604
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleLeukemia cells
Sample nameK562
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
41
Experiment ID605
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleLeukemia cells
Sample nameMOLT-4
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
42
Experiment ID607
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleLeukemia cells
Sample nameSR
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
43
Experiment ID612
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleLung cancer cells
Sample nameNCI-H226
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
44
Experiment ID614
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleLung cancer cells
Sample nameNCI-H322M
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
45
Experiment ID616
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleLung cancer cells
Sample nameNCI-H522
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
46
Experiment ID126
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Antibody array
PubMed ID Unpublished / Not applicable
OrganismHomo sapiens
Experiment descriptionMesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
AuthorsRuenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year2011
SampleMesenchymal stem cells
Sample namehuES9.E1
Isolation/purification methodsHPLC
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Antibody array
Mass spectrometry
EV-TRACK -
47
Experiment ID244
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry[Q-TOF]
PubMed ID 22148876   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of microvesicles derived from human mesenchymal stem cells
AuthorsKim HS, Choi DY, Yun SJ, Choi SM, Kang JW, Jung JW, Hwang D, Kim KP, Kim DW
Journal name J Proteome Res
Publication year2011
SampleMesenchymal stem cells
Sample nameMesenchymal stem cells - Rep 1
Isolation/purification methodsDifferential centrifugation
Ultrafiltration
Ultracentrifugation
Density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry[QTOF]
EV-TRACK -
48
Experiment ID310
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry[Q-TOF]
PubMed ID 22148876   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of microvesicles derived from human mesenchymal stem cells
AuthorsKim HS, Choi DY, Yun SJ, Choi SM, Kang JW, Jung JW, Hwang D, Kim KP, Kim DW
Journal name J Proteome Res
Publication year2011
SampleMesenchymal stem cells
Sample nameMesenchymal stem cells - Rep 2
Isolation/purification methodsDifferential centrifugation
Ultrafiltration
Ultracentrifugation
Density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry[QTOF]
EV-TRACK -
49
Experiment ID401
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry
PubMed ID 19548909   
OrganismHomo sapiens
Experiment descriptionDifferential stimulation of monocytic cells results in distinct populations of microparticles.
AuthorsBernimoulin M, Waters EK, Foy M, Steele BM, Sullivan M, Falet H, Walsh MT, Barteneva N, Geng JG, Hartwig JH, Maguire PB, Wagner DD.
Journal name J Thromb Haemost
Publication year2009
SampleMonocytes
Sample nameMonocytes (THP-1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
50
Experiment ID402
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry
PubMed ID 19548909   
OrganismHomo sapiens
Experiment descriptionDifferential stimulation of monocytic cells results in distinct populations of microparticles.
AuthorsBernimoulin M, Waters EK, Foy M, Steele BM, Sullivan M, Falet H, Walsh MT, Barteneva N, Geng JG, Hartwig JH, Maguire PB, Wagner DD.
Journal name J Thromb Haemost
Publication year2009
SampleMonocytes
Sample nameStimulated by IgG-Monocytes (THP-1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
51
Experiment ID403
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry
PubMed ID 19548909   
OrganismHomo sapiens
Experiment descriptionDifferential stimulation of monocytic cells results in distinct populations of microparticles.
AuthorsBernimoulin M, Waters EK, Foy M, Steele BM, Sullivan M, Falet H, Walsh MT, Barteneva N, Geng JG, Hartwig JH, Maguire PB, Wagner DD.
Journal name J Thromb Haemost
Publication year2009
SampleMonocytes
Sample nameStimulated by LPS-Monocytes (THP-1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
52
Experiment ID404
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry
PubMed ID 19548909   
OrganismHomo sapiens
Experiment descriptionDifferential stimulation of monocytic cells results in distinct populations of microparticles.
AuthorsBernimoulin M, Waters EK, Foy M, Steele BM, Sullivan M, Falet H, Walsh MT, Barteneva N, Geng JG, Hartwig JH, Maguire PB, Wagner DD.
Journal name J Thromb Haemost
Publication year2009
SampleMonocytes
Sample nameStimulated by P-selectin-Ig-Monocytes (THP-1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
53
Experiment ID467
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles/Nanovesicles
Identification method Mass spectrometry
PubMed ID 23000592   
OrganismHomo sapiens
Experiment descriptionIn-depth analysis of the secretome identifies three major independent secretory pathways in differentiating human myoblasts.
AuthorsLe Bihan MC, Bigot A, Jensen SS, Dennis J, Rogowska-Wrzesinska A, Lain챕 J, Gache V, Furling D, Jensen ON, Voit T, Mouly V, Coulton GR, Butler-Browne G.
Journal name J Proteomics
Publication year2012
SampleNeonatal myoblast cells
Sample namePatient of developmental heart defect_exosome-Neonatal myoblast cells
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
54
Experiment ID468
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles/Nanovesicles
Identification method Mass spectrometry
PubMed ID 23000592   
OrganismHomo sapiens
Experiment descriptionIn-depth analysis of the secretome identifies three major independent secretory pathways in differentiating human myoblasts.
AuthorsLe Bihan MC, Bigot A, Jensen SS, Dennis J, Rogowska-Wrzesinska A, Lain챕 J, Gache V, Furling D, Jensen ON, Voit T, Mouly V, Coulton GR, Butler-Browne G.
Journal name J Proteomics
Publication year2012
SampleNeonatal myoblast cells
Sample namePatient of developmental heart defect_microparticle-Neonatal myoblast cells
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
55
Experiment ID492
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23333927   
OrganismHomo sapiens
Experiment descriptionCharacterization and proteomic analysis of ovarian cancer-derived exosomes.
AuthorsLiang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year2013
SampleOvarian cancer cells
Sample nameIGROV1
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130002: EV-METRIC:67%, 56%
56
Experiment ID493
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23333927   
OrganismHomo sapiens
Experiment descriptionCharacterization and proteomic analysis of ovarian cancer-derived exosomes.
AuthorsLiang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year2013
SampleOvarian cancer cells
Sample nameOVCAR-3
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130002: EV-METRIC:67%, 56%
57
Experiment ID626
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleOvarian cancer cells
Sample nameIGROV1
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
58
Experiment ID628
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleOvarian cancer cells
Sample nameOVCAR-4
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
59
Experiment ID632
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleOvarian cancer cells
Sample nameNCI-ADR-RES
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
60
Experiment ID992
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 24434149   
OrganismHomo sapiens
Experiment descriptionIn-depth proteomic analyses of ovarian cancer cell line exosomes reveals differential enrichment of functional categories compared to the NCI 60 proteome.
AuthorsSinha A, Ignatchenko V, Ignatchenko A, Mejia-Guerrero S, Kislinger T.
Journal name Biochem Biophys Res Commun.
Publication year2014
SampleOvarian cancer cells
Sample nameOVCAR3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV140099: EV-METRIC:44%
61
Experiment ID993
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 24434149   
OrganismHomo sapiens
Experiment descriptionIn-depth proteomic analyses of ovarian cancer cell line exosomes reveals differential enrichment of functional categories compared to the NCI 60 proteome.
AuthorsSinha A, Ignatchenko V, Ignatchenko A, Mejia-Guerrero S, Kislinger T.
Journal name Biochem Biophys Res Commun.
Publication year2014
SampleOvarian cancer cells
Sample nameOVCAR433
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV140099: EV-METRIC:44%
62
Experiment ID994
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 24434149   
OrganismHomo sapiens
Experiment descriptionIn-depth proteomic analyses of ovarian cancer cell line exosomes reveals differential enrichment of functional categories compared to the NCI 60 proteome.
AuthorsSinha A, Ignatchenko V, Ignatchenko A, Mejia-Guerrero S, Kislinger T.
Journal name Biochem Biophys Res Commun.
Publication year2014
SampleOvarian cancer cells
Sample nameOVCAR5
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV140099: EV-METRIC:44%
63
Experiment ID995
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 24434149   
OrganismHomo sapiens
Experiment descriptionIn-depth proteomic analyses of ovarian cancer cell line exosomes reveals differential enrichment of functional categories compared to the NCI 60 proteome.
AuthorsSinha A, Ignatchenko V, Ignatchenko A, Mejia-Guerrero S, Kislinger T.
Journal name Biochem Biophys Res Commun.
Publication year2014
SampleOvarian cancer cells
Sample nameSKOV3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV140099: EV-METRIC:44%
64
Experiment ID633
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleProstate cancer cells
Sample nameDU145
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
65
Experiment ID963
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 25844599   
OrganismHomo sapiens
Experiment descriptionMolecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
AuthorsKharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, UlléA, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year2015
SampleProstate cancer cells
Sample nameDU145 Tax-Sen
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12-1.19 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV150027: EV-METRIC:11%, 56%
66
Experiment ID964
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 25844599   
OrganismHomo sapiens
Experiment descriptionMolecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
AuthorsKharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, UlléA, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year2015
SampleProstate cancer cells
Sample nameDU145 Tax-Res
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.13-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV150027: EV-METRIC:11%, 56%
67
Experiment ID538
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23844026   
OrganismHomo sapiens
Experiment descriptionCharacterization of human thymic exosomes.
AuthorsSkogberg G, Gudmundsdottir J, van der Post S, Sandström K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year2013
SampleThymus
Sample nameNormal-Thymus
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130018: EV-METRIC:63%
68
Experiment ID680
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry
PubMed ID 29148239   
OrganismHomo sapiens
Experiment descriptionMetabolic Signature of Microvesicles from Umbilical Cord Mesenchymal Stem Cells of Preterm and Term Infants.
AuthorsBruschi M, Santucci L, Ravera S, Bartolucci M, Petretto A, Calzia D, Ghiggeri GM, Ramenghi LA, Candiano G, Panfoli I.
Journal name Proteomics Clin Appl
Publication year2018
SampleUmblical cord mesenchymal stem cells
Sample namePreterm newborns- replicate 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Sonication
Filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
69
Experiment ID681
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry
PubMed ID 29148239   
OrganismHomo sapiens
Experiment descriptionMetabolic Signature of Microvesicles from Umbilical Cord Mesenchymal Stem Cells of Preterm and Term Infants.
AuthorsBruschi M, Santucci L, Ravera S, Bartolucci M, Petretto A, Calzia D, Ghiggeri GM, Ramenghi LA, Candiano G, Panfoli I.
Journal name Proteomics Clin Appl
Publication year2018
SampleUmblical cord mesenchymal stem cells
Sample namePreterm newborns- replicate 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Sonication
Filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
70
Experiment ID682
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry
PubMed ID 29148239   
OrganismHomo sapiens
Experiment descriptionMetabolic Signature of Microvesicles from Umbilical Cord Mesenchymal Stem Cells of Preterm and Term Infants.
AuthorsBruschi M, Santucci L, Ravera S, Bartolucci M, Petretto A, Calzia D, Ghiggeri GM, Ramenghi LA, Candiano G, Panfoli I.
Journal name Proteomics Clin Appl
Publication year2018
SampleUmblical cord mesenchymal stem cells
Sample namePreterm newborns- replicate 3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Sonication
Filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
71
Experiment ID683
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry
PubMed ID 29148239   
OrganismHomo sapiens
Experiment descriptionMetabolic Signature of Microvesicles from Umbilical Cord Mesenchymal Stem Cells of Preterm and Term Infants.
AuthorsBruschi M, Santucci L, Ravera S, Bartolucci M, Petretto A, Calzia D, Ghiggeri GM, Ramenghi LA, Candiano G, Panfoli I.
Journal name Proteomics Clin Appl
Publication year2018
SampleUmblical cord mesenchymal stem cells
Sample nameTerm infants- replicate 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Sonication
Filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
72
Experiment ID684
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry
PubMed ID 29148239   
OrganismHomo sapiens
Experiment descriptionMetabolic Signature of Microvesicles from Umbilical Cord Mesenchymal Stem Cells of Preterm and Term Infants.
AuthorsBruschi M, Santucci L, Ravera S, Bartolucci M, Petretto A, Calzia D, Ghiggeri GM, Ramenghi LA, Candiano G, Panfoli I.
Journal name Proteomics Clin Appl
Publication year2018
SampleUmblical cord mesenchymal stem cells
Sample nameTerm infants- replicate 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Sonication
Filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
73
Experiment ID685
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry
PubMed ID 29148239   
OrganismHomo sapiens
Experiment descriptionMetabolic Signature of Microvesicles from Umbilical Cord Mesenchymal Stem Cells of Preterm and Term Infants.
AuthorsBruschi M, Santucci L, Ravera S, Bartolucci M, Petretto A, Calzia D, Ghiggeri GM, Ramenghi LA, Candiano G, Panfoli I.
Journal name Proteomics Clin Appl
Publication year2018
SampleUmblical cord mesenchymal stem cells
Sample nameTerm infants- replicate 3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Sonication
Filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
74
Experiment ID63
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 19056867   
OrganismHomo sapiens
Experiment descriptionLarge-scale proteomics and phosphoproteomics of urinary exosomes.
AuthorsGonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year2009
SampleUrine
Sample nameUrine - Normal
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
Western blotting
EV-TRACK -
75
Experiment ID437
Identified moleculeprotein
Extracellular vesicle typeExosomes/Membrane vesicles
Identification method Mass spectrometry
PubMed ID 22106071   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of urine exosomes by multidimensional protein identification technology (MudPIT).
AuthorsWang Z, Hill S, Luther JM, Hachey DL, Schey KL.
Journal name Proteomics
Publication year2012
SampleUrine
Sample nameNormal donors-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120168: EV-METRIC:14%