Gene description for Uba1
Gene name ubiquitin-like modifier activating enzyme 1
Gene symbol Uba1
Other names/aliases A1S9
AA989744
Sbx
Ube-1
Ube1x
Species Mus musculus
 Database cross references - Uba1
Vesiclepedia VP_22201
ExoCarta ExoCarta_22201
Entrez Gene 22201
 Uba1 identified in extracellular vesicles derived from the following tissue/cell type
Adipocytes [Microvesicles]      More >>> 17478559   
Breast cancer cells [Exosomes]      More >>> 29115712   
Breast cancer cells [Exosomes]      More >>> 29115712   
Breast cancer cells [Exosomes]      More >>> 29115712   
Breast cancer cells [Exosomes]      More >>> 29115712   
Macrophages [Exosomes]      More >>> 23658846   
Macrophages [Exosomes]      More >>> 23658846   
Macrophages [Exosomes]      More >>> 23658846   
Mouse embryonic fibroblasts [Extracellular vesicles]      More >>> 30035390   
Mouse embryonic fibroblasts [Extracellular vesicles]      More >>> 30035390   
Mouse embryonic fibroblasts [Extracellular vesicles]      More >>> 30035390   
Mouse embryonic fibroblasts [Extracellular vesicles]      More >>> 30035390   
Murine myotubues [Extracellular vesicles]      More >>> 24392111   
Myeloid-derived suppressor cells [Exosomes]      More >>> 29951053   
Myeloid-derived suppressor cells [Exosomes]      More >>> 29951053   
Myeloid-derived suppressor cells [Exosomes]      More >>> 24295599   
Myeloid-derived suppressor cells [Exosomes]      More >>> 24295599   
Pancreatic cells [Exosomes]      More >>> 19351151   
T cells [Exosomes]      More >>> 23463506   
 Experiment description of studies that identified Uba1 in extracellular vesicles
1
Experiment ID304
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry [LCQ-DECA XP]
PubMed ID 17478559   
OrganismMus musculus
Experiment descriptionIdentification and characterization of microvesicles secreted by 3T3-L1 adipocytes: redox- and hormone-dependent induction of milk fat globule-epidermal growth 8-associated microvesicles
AuthorsAoki N, Jin-no S, Nakagawa Y, Asai N, Arakawa E, Tamura N, Tamura T, Matsuda T
Journal name Endocrinology
Publication year2007
SampleAdipocytes
Sample name3T3-L1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.13–-1.20 g/mL
Molecules identified in the studyProtein
Methods used in the studyImmunoelectron microscopy
Western blotting
Mass spectrometry [LCQ-DECA XP]
EV-TRACK -
2
Experiment ID1051
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 29115712   
OrganismMus musculus
Experiment descriptionProteomic Profiling of Exosomes Secreted by Breast Cancer Cells with Varying Metastatic Potential.
AuthorsGangoda L, Liem M, Ang CS, Keerthikumar S, Adda CG, Parker BS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleBreast cancer cells
Sample name67NR
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
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3
Experiment ID1052
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 29115712   
OrganismMus musculus
Experiment descriptionProteomic Profiling of Exosomes Secreted by Breast Cancer Cells with Varying Metastatic Potential.
AuthorsGangoda L, Liem M, Ang CS, Keerthikumar S, Adda CG, Parker BS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleBreast cancer cells
Sample name66cl4
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
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4
Experiment ID1053
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 29115712   
OrganismMus musculus
Experiment descriptionProteomic Profiling of Exosomes Secreted by Breast Cancer Cells with Varying Metastatic Potential.
AuthorsGangoda L, Liem M, Ang CS, Keerthikumar S, Adda CG, Parker BS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleBreast cancer cells
Sample name4T1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
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5
Experiment ID1054
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 29115712   
OrganismMus musculus
Experiment descriptionProteomic Profiling of Exosomes Secreted by Breast Cancer Cells with Varying Metastatic Potential.
AuthorsGangoda L, Liem M, Ang CS, Keerthikumar S, Adda CG, Parker BS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleBreast cancer cells
Sample name4T1.2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
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6
Experiment ID523
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23658846   
OrganismMus musculus
Experiment descriptionImmunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
AuthorsHassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year2013
SampleMacrophages
Sample nameLeishmania-infected-Macrophage (J774A.1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130144: EV-METRIC:0%, 33%
7
Experiment ID524
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23658846   
OrganismMus musculus
Experiment descriptionImmunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
AuthorsHassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year2013
SampleMacrophages
Sample nameLPS-treated-Macrophage (J774A.1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130144: EV-METRIC:0%, 33%
8
Experiment ID525
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23658846   
OrganismMus musculus
Experiment descriptionImmunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
AuthorsHassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year2013
SampleMacrophages
Sample nameNormal-Macrophage (J774A.1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130144: EV-METRIC:0%, 33%
9
Experiment ID1055
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30035390   
OrganismMus musculus
Experiment descriptionArrestin-domain Containing Protein 1 (Arrdc1) Regulates the Protein Cargo and Release of Extracellular Vesicles.
AuthorsAnand S, Foot N, Ang CS, Gembus KM, Keerthikumar S, Adda CG, Mathivanan S, Kumar S.
Journal name Proteomics.
Publication year2018
SampleMouse embryonic fibroblasts
Sample nameMEFs - WT 10K
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
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10
Experiment ID1056
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30035390   
OrganismMus musculus
Experiment descriptionArrestin-domain Containing Protein 1 (Arrdc1) Regulates the Protein Cargo and Release of Extracellular Vesicles.
AuthorsAnand S, Foot N, Ang CS, Gembus KM, Keerthikumar S, Adda CG, Mathivanan S, Kumar S.
Journal name Proteomics.
Publication year2018
SampleMouse embryonic fibroblasts
Sample nameMEFs - WT exosomes
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
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11
Experiment ID1057
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30035390   
OrganismMus musculus
Experiment descriptionArrestin-domain Containing Protein 1 (Arrdc1) Regulates the Protein Cargo and Release of Extracellular Vesicles.
AuthorsAnand S, Foot N, Ang CS, Gembus KM, Keerthikumar S, Adda CG, Mathivanan S, Kumar S.
Journal name Proteomics.
Publication year2018
SampleMouse embryonic fibroblasts
Sample nameMEFs - Arrdc1 KO 10K
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
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12
Experiment ID1058
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30035390   
OrganismMus musculus
Experiment descriptionArrestin-domain Containing Protein 1 (Arrdc1) Regulates the Protein Cargo and Release of Extracellular Vesicles.
AuthorsAnand S, Foot N, Ang CS, Gembus KM, Keerthikumar S, Adda CG, Mathivanan S, Kumar S.
Journal name Proteomics.
Publication year2018
SampleMouse embryonic fibroblasts
Sample nameMEFs - Arrdc1 KO exosomes
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
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13
Experiment ID997
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 24392111   
OrganismMus musculus
Experiment descriptionProteomic analysis of C2C12 myoblast and myotube exosome-like vesicles: a new paradigm for myoblast-myotube cross talk?
AuthorsForterre A, Jalabert A, Berger E, Baudet M, Chikh K, Errazuriz E, De Larichaudy J, Chanon S, Weiss-Gayet M, Hesse AM, Record M, Geloen A, Lefai E, Vidal H, Couté, Rome S.
Journal name PLoS One.
Publication year2014
SampleMurine myotubues
Sample nameC2C12 - MT
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV140002: EV-METRIC:67%
14
Experiment ID678
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 29951053   
OrganismMus musculus
Experiment descriptionImmunoregulatory Effects of Myeloid-Derived Suppressor Cell Exosomes in Mouse Model of Autoimmune Alopecia Areata.
AuthorsZör M, Zhao K, Kutlu N, Bauer N, Provaznik J, Hackert T, Schnör M.
Journal name Front Immunol
Publication year2018
SampleMyeloid-derived suppressor cells
Sample nameIn-vitro- Alopecia areata affected C3H/HeJ mice BMC derived
Isolation/purification methodsUltracentrifugation
Density gradient centrifugation
Flotation densityNot provided
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
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15
Experiment ID679
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 29951053   
OrganismMus musculus
Experiment descriptionImmunoregulatory Effects of Myeloid-Derived Suppressor Cell Exosomes in Mouse Model of Autoimmune Alopecia Areata.
AuthorsZör M, Zhao K, Kutlu N, Bauer N, Provaznik J, Hackert T, Schnör M.
Journal name Front Immunol
Publication year2018
SampleMyeloid-derived suppressor cells
Sample nameEx-vivo- Alopecia areata affected C3H/HeJ mice
Isolation/purification methodsUltracentrifugation
Density gradient centrifugation
Flotation densityNot provided
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
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16
Experiment ID1126
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 24295599   
OrganismMus musculus
Experiment descriptionExosomes from myeloid-derived suppressor cells carry biologically active proteins.
AuthorsBurke M, Choksawangkarn W, Edwards N, Ostrand-Rosenberg S, Fenselau C.
Journal name J Proteome Res
Publication year2014
SampleMyeloid-derived suppressor cells
Sample nameConventional MDSC's-BALB/c mice injected with 4T1 cells
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140063: EV-METRIC:71%
17
Experiment ID1127
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 24295599   
OrganismMus musculus
Experiment descriptionExosomes from myeloid-derived suppressor cells carry biologically active proteins.
AuthorsBurke M, Choksawangkarn W, Edwards N, Ostrand-Rosenberg S, Fenselau C.
Journal name J Proteome Res
Publication year2014
SampleMyeloid-derived suppressor cells
Sample nameInflammatory MDSC's-BALB/c mice injected with 4T1 cells expressing IL-1.
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140063: EV-METRIC:71%
18
Experiment ID392
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 19351151   
OrganismMus musculus
Experiment descriptionCharacterization of vesicles secreted from insulinoma NIT-1 cells.
AuthorsLee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year2009
SamplePancreatic cells
Sample namePancreatic beta cell (NIT-1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
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19
Experiment ID1007
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23463506   
OrganismMus musculus
Experiment descriptionThe intracellular interactome of tetraspanin-enriched microdomains reveals their function as sorting machineries toward exosomes.
AuthorsPerez-Hernandez D, Gutiéez-Váuez C, Jorge I, Ló-MartíS, Ursa A, Sáhez-Madrid F, Váuez J, Yáz-Mó
Journal name J Biol Chem.
Publication year2013
SampleT cells
Sample nameT lymphoblasts - CD81 KO mice
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV130110: EV-METRIC:22%